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ATP-Mediated Compositional Change in Peripheral Myelin Membranes: A Comparative Raman Spectroscopy and Time-Of-Flight Secondary Ion Mass Spectrometry Study.

Kutuzov N, Gulin A, Lyaskovskiy V, Nadtochenko V, Maksimov G - PLoS ONE (2015)

Bottom Line: The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine.Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts.Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

View Article: PubMed Central - PubMed

Affiliation: Biophysics Department, Biological Faculty, Moscow State University, Leninskie gory 1/12, Moscow, Russian Federation, 119991.

ABSTRACT
In the present paper we addressed a mechanism of the myelin reorganization initiated by extracellular ATP and adenosine in sciatic nerves of the frog Rana temporaria. In combination with Raman microspectroscopy, allowing noninvasive live-cell measurements, we employed time-of-flight secondary ion mass spectrometry (TOF-SIMS) to follow the underlying changes in chemical composition of myelin membranes triggered by the purinergic agents. The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine. Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts. Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

No MeSH data available.


The comparison of Raman intensity ratios after ATP, adenosine and MBCD administration.The corresponding concentrations are shown on histogram bars. Asterisks * and ** denote statistic p < 0.05 and p < 0.001 respectively.
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pone.0142084.g002: The comparison of Raman intensity ratios after ATP, adenosine and MBCD administration.The corresponding concentrations are shown on histogram bars. Asterisks * and ** denote statistic p < 0.05 and p < 0.001 respectively.

Mentions: The administration of both ATP and adenosine in concentration of 1 mM resulted in increased ordering degree of lipids and membrane viscosity, which is clear from the elevation of Rcar and Rlip ratios. Oppositely, as shown in Fig 2(a) and 2(b), Rsat was decreased implying the elevated amount of saturated fatty acids. When we lowered the concentration of ATP and adenosine down to 0.25 mM we saw no significant differences in any ratios Fig 2(a) and 2(b).


ATP-Mediated Compositional Change in Peripheral Myelin Membranes: A Comparative Raman Spectroscopy and Time-Of-Flight Secondary Ion Mass Spectrometry Study.

Kutuzov N, Gulin A, Lyaskovskiy V, Nadtochenko V, Maksimov G - PLoS ONE (2015)

The comparison of Raman intensity ratios after ATP, adenosine and MBCD administration.The corresponding concentrations are shown on histogram bars. Asterisks * and ** denote statistic p < 0.05 and p < 0.001 respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4636249&req=5

pone.0142084.g002: The comparison of Raman intensity ratios after ATP, adenosine and MBCD administration.The corresponding concentrations are shown on histogram bars. Asterisks * and ** denote statistic p < 0.05 and p < 0.001 respectively.
Mentions: The administration of both ATP and adenosine in concentration of 1 mM resulted in increased ordering degree of lipids and membrane viscosity, which is clear from the elevation of Rcar and Rlip ratios. Oppositely, as shown in Fig 2(a) and 2(b), Rsat was decreased implying the elevated amount of saturated fatty acids. When we lowered the concentration of ATP and adenosine down to 0.25 mM we saw no significant differences in any ratios Fig 2(a) and 2(b).

Bottom Line: The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine.Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts.Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

View Article: PubMed Central - PubMed

Affiliation: Biophysics Department, Biological Faculty, Moscow State University, Leninskie gory 1/12, Moscow, Russian Federation, 119991.

ABSTRACT
In the present paper we addressed a mechanism of the myelin reorganization initiated by extracellular ATP and adenosine in sciatic nerves of the frog Rana temporaria. In combination with Raman microspectroscopy, allowing noninvasive live-cell measurements, we employed time-of-flight secondary ion mass spectrometry (TOF-SIMS) to follow the underlying changes in chemical composition of myelin membranes triggered by the purinergic agents. The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine. Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts. Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

No MeSH data available.