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ATP-Mediated Compositional Change in Peripheral Myelin Membranes: A Comparative Raman Spectroscopy and Time-Of-Flight Secondary Ion Mass Spectrometry Study.

Kutuzov N, Gulin A, Lyaskovskiy V, Nadtochenko V, Maksimov G - PLoS ONE (2015)

Bottom Line: The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine.Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts.Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

View Article: PubMed Central - PubMed

Affiliation: Biophysics Department, Biological Faculty, Moscow State University, Leninskie gory 1/12, Moscow, Russian Federation, 119991.

ABSTRACT
In the present paper we addressed a mechanism of the myelin reorganization initiated by extracellular ATP and adenosine in sciatic nerves of the frog Rana temporaria. In combination with Raman microspectroscopy, allowing noninvasive live-cell measurements, we employed time-of-flight secondary ion mass spectrometry (TOF-SIMS) to follow the underlying changes in chemical composition of myelin membranes triggered by the purinergic agents. The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine. Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts. Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

No MeSH data available.


Myelin Raman spectra with main peaks and intensity ratios used for calculations.
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pone.0142084.g001: Myelin Raman spectra with main peaks and intensity ratios used for calculations.

Mentions: Typical Raman spectra of a nerve fiber consists of carotenoid and lipid bands (Fig 1), assignment of which was established previously [16]. Carotenoid Raman bands include 1160 cm−1 and 1520 cm−1 peaks corresponding to -C–C- and -C = C- band vibrations [23, 24]. Peaks located near 1440 cm−1 and 1668 cm−1 are attributed to CH2 bend and -C = C- stretch modes of fatty acids [25]. Finally, 2884 cm−1 and 2935 cm−1 can be assigned respectively to asymmetric methylene stretching vibration and symmetric stretching of terminal methyl groups [26].


ATP-Mediated Compositional Change in Peripheral Myelin Membranes: A Comparative Raman Spectroscopy and Time-Of-Flight Secondary Ion Mass Spectrometry Study.

Kutuzov N, Gulin A, Lyaskovskiy V, Nadtochenko V, Maksimov G - PLoS ONE (2015)

Myelin Raman spectra with main peaks and intensity ratios used for calculations.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4636249&req=5

pone.0142084.g001: Myelin Raman spectra with main peaks and intensity ratios used for calculations.
Mentions: Typical Raman spectra of a nerve fiber consists of carotenoid and lipid bands (Fig 1), assignment of which was established previously [16]. Carotenoid Raman bands include 1160 cm−1 and 1520 cm−1 peaks corresponding to -C–C- and -C = C- band vibrations [23, 24]. Peaks located near 1440 cm−1 and 1668 cm−1 are attributed to CH2 bend and -C = C- stretch modes of fatty acids [25]. Finally, 2884 cm−1 and 2935 cm−1 can be assigned respectively to asymmetric methylene stretching vibration and symmetric stretching of terminal methyl groups [26].

Bottom Line: The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine.Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts.Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

View Article: PubMed Central - PubMed

Affiliation: Biophysics Department, Biological Faculty, Moscow State University, Leninskie gory 1/12, Moscow, Russian Federation, 119991.

ABSTRACT
In the present paper we addressed a mechanism of the myelin reorganization initiated by extracellular ATP and adenosine in sciatic nerves of the frog Rana temporaria. In combination with Raman microspectroscopy, allowing noninvasive live-cell measurements, we employed time-of-flight secondary ion mass spectrometry (TOF-SIMS) to follow the underlying changes in chemical composition of myelin membranes triggered by the purinergic agents. The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine. Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts. Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

No MeSH data available.