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Identification of Putative Olfactory Genes from the Oriental Fruit Moth Grapholita molesta via an Antennal Transcriptome Analysis.

Li G, Du J, Li Y, Wu J - PLoS ONE (2015)

Bottom Line: The identified genes were further confirmed through semi-quantitative reverse transcription PCR for transcripts coding for 26 OBPs and 17 CSPs.OBP transcripts showed an obvious antenna bias, whereas CSP transcripts were detected in different tissues.Antennal transcriptome data derived from the oriental fruit moth constituted an abundant molecular resource for the identification of genes potentially involved in the olfaction process of the species.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Protection Resources and Pest Management (Northwest A&F University), Ministry of Education, Yangling, Shaanxi, People's Republic of China.

ABSTRACT

Background: The oriental fruit moth, Grapholita molesta, is an extremely important oligophagous pest species of stone and pome fruits throughout the world. As a host-switching species, adult moths, especially females, depend on olfactory cues to a large extent in locating host plants, finding mates, and selecting oviposition sites. The identification of olfactory genes can facilitate investigation on mechanisms for chemical communications.

Methodology/principal finding: We generated transcriptome of female antennae of G.molesta using the next-generation sequencing technique, and assembled transcripts from RNA-seq reads using Trinity, SOAPdenovo-trans and Abyss-trans assemblers. We identified 124 putative olfactory genes. Among the identified olfactory genes, 118 were novel to this species, including 28 transcripts encoding for odorant binding proteins, 17 chemosensory proteins, 48 odorant receptors, four gustatory receptors, 24 ionotropic receptors, two sensory neuron membrane proteins, and one odor degrading enzyme. The identified genes were further confirmed through semi-quantitative reverse transcription PCR for transcripts coding for 26 OBPs and 17 CSPs. OBP transcripts showed an obvious antenna bias, whereas CSP transcripts were detected in different tissues.

Conclusion: Antennal transcriptome data derived from the oriental fruit moth constituted an abundant molecular resource for the identification of genes potentially involved in the olfaction process of the species. This study provides a foundation for future research on the molecules involved in olfactory recognition of this insect pest, and in particular, the feasibility of using semiochemicals to control this pest.

No MeSH data available.


Related in: MedlinePlus

Gene Ontology (GO) analysis of G. molesta antennal transcripts.GO terms assigned to biological process, cellular component and molecular functions.
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pone.0142193.g003: Gene Ontology (GO) analysis of G. molesta antennal transcripts.GO terms assigned to biological process, cellular component and molecular functions.

Mentions: A total of 16,215 unigenes matched to known proteins in Genbank. Among the annotated unigenes, 64.3% had a first hits to Lepidopteran sequences. The top matched species were Danaus plexippus (52.2%), Bombyx mori (6.5%), Tribolium castaneum (4.4%), Papilio xuthus (4.1%), and Acyrthosiphon pisum (1.5%) (Fig 2). Fig 3 illustrated the distribution of the unigenes in GO terms. Among the 16,215 unigenes, 11,569 (71.35%) were assigned to 55,382 GO term annotations, with biological processes 26,297 terms; molecular function 15,062 terms; and cellular component 14,023 terms. In the biological process terms, transport, signal transduction and oxidoreductase activity were the mostly represented, while in the cellular component terms, the cytoplasm and intracellular were the most abundant. In the molecular function category, the genes expressed in the antennae were mostly enriched to DNA binding and RNA binding activity.


Identification of Putative Olfactory Genes from the Oriental Fruit Moth Grapholita molesta via an Antennal Transcriptome Analysis.

Li G, Du J, Li Y, Wu J - PLoS ONE (2015)

Gene Ontology (GO) analysis of G. molesta antennal transcripts.GO terms assigned to biological process, cellular component and molecular functions.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4635014&req=5

pone.0142193.g003: Gene Ontology (GO) analysis of G. molesta antennal transcripts.GO terms assigned to biological process, cellular component and molecular functions.
Mentions: A total of 16,215 unigenes matched to known proteins in Genbank. Among the annotated unigenes, 64.3% had a first hits to Lepidopteran sequences. The top matched species were Danaus plexippus (52.2%), Bombyx mori (6.5%), Tribolium castaneum (4.4%), Papilio xuthus (4.1%), and Acyrthosiphon pisum (1.5%) (Fig 2). Fig 3 illustrated the distribution of the unigenes in GO terms. Among the 16,215 unigenes, 11,569 (71.35%) were assigned to 55,382 GO term annotations, with biological processes 26,297 terms; molecular function 15,062 terms; and cellular component 14,023 terms. In the biological process terms, transport, signal transduction and oxidoreductase activity were the mostly represented, while in the cellular component terms, the cytoplasm and intracellular were the most abundant. In the molecular function category, the genes expressed in the antennae were mostly enriched to DNA binding and RNA binding activity.

Bottom Line: The identified genes were further confirmed through semi-quantitative reverse transcription PCR for transcripts coding for 26 OBPs and 17 CSPs.OBP transcripts showed an obvious antenna bias, whereas CSP transcripts were detected in different tissues.Antennal transcriptome data derived from the oriental fruit moth constituted an abundant molecular resource for the identification of genes potentially involved in the olfaction process of the species.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Protection Resources and Pest Management (Northwest A&F University), Ministry of Education, Yangling, Shaanxi, People's Republic of China.

ABSTRACT

Background: The oriental fruit moth, Grapholita molesta, is an extremely important oligophagous pest species of stone and pome fruits throughout the world. As a host-switching species, adult moths, especially females, depend on olfactory cues to a large extent in locating host plants, finding mates, and selecting oviposition sites. The identification of olfactory genes can facilitate investigation on mechanisms for chemical communications.

Methodology/principal finding: We generated transcriptome of female antennae of G.molesta using the next-generation sequencing technique, and assembled transcripts from RNA-seq reads using Trinity, SOAPdenovo-trans and Abyss-trans assemblers. We identified 124 putative olfactory genes. Among the identified olfactory genes, 118 were novel to this species, including 28 transcripts encoding for odorant binding proteins, 17 chemosensory proteins, 48 odorant receptors, four gustatory receptors, 24 ionotropic receptors, two sensory neuron membrane proteins, and one odor degrading enzyme. The identified genes were further confirmed through semi-quantitative reverse transcription PCR for transcripts coding for 26 OBPs and 17 CSPs. OBP transcripts showed an obvious antenna bias, whereas CSP transcripts were detected in different tissues.

Conclusion: Antennal transcriptome data derived from the oriental fruit moth constituted an abundant molecular resource for the identification of genes potentially involved in the olfaction process of the species. This study provides a foundation for future research on the molecules involved in olfactory recognition of this insect pest, and in particular, the feasibility of using semiochemicals to control this pest.

No MeSH data available.


Related in: MedlinePlus