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Molecular Characterization of Human Respiratory Syncytial Virus in the Philippines, 2012-2013.

Malasao R, Okamoto M, Chaimongkol N, Imamura T, Tohma K, Dapat I, Dapat C, Suzuki A, Saito M, Saito M, Tamaki R, Pedrera-Rico GA, Aniceto R, Quicho RF, Segubre-Mercado E, Lupisan S, Oshitani H - PLoS ONE (2015)

Bottom Line: Moreover, in the ON1 genotype, we found positive selection at amino acid position 274 (p<0.05) and massive O- and N-glycosylation in the 2nd HVR of the G gene.However, two sporadic cases of GB2 genotype were found, which might share a common ancestor with other Asian strains.Both genotypes were detected for the first time in the Philippines.

View Article: PubMed Central - PubMed

Affiliation: Tohoku University Graduate School of Medicine, Sendai, Japan.

ABSTRACT
Human respiratory syncytial virus (HRSV) is a major cause of acute lower respiratory tract infections in infants and children worldwide. We performed molecular analysis of HRSV among infants and children with clinical diagnosis of severe pneumonia in four study sites in the Philippines, including Biliran, Leyte, Palawan, and Metro Manila from June 2012 to July 2013. Nasopharyngeal swabs were collected and screened for HRSV using real-time polymerase chain reaction (PCR). Positive samples were tested by conventional PCR and sequenced for the second hypervariable region (2nd HVR) of the G gene. Among a total of 1,505 samples, 423 samples were positive for HRSV (28.1%), of which 305 (72.1%) and 118 (27.9%) were identified as HRSV-A and HRSV-B, respectively. Two genotypes of HRSV-A, NA1 and ON1, were identified during the study period. The novel ON1 genotype with a 72-nucleotide duplication in 2nd HVR of the G gene increased rapidly and finally became the predominant genotype in 2013 with an evolutionary rate higher than the NA1 genotype. Moreover, in the ON1 genotype, we found positive selection at amino acid position 274 (p<0.05) and massive O- and N-glycosylation in the 2nd HVR of the G gene. Among HRSV-B, BA9 was the predominant genotype circulating in the Philippines. However, two sporadic cases of GB2 genotype were found, which might share a common ancestor with other Asian strains. These findings suggest that HRSV is an important cause of severe acute respiratory infection among children in the Philippines and revealed the emergence and subsequent predominance of the ON1 genotype and the sporadic detection of the GB2 genotype. Both genotypes were detected for the first time in the Philippines.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis of GB2 genotype from the Philippines.The phylogenetic tree was constructed by adding 98 strains from other countries that were identified by BLASTn search. The Philippine GB2 strains may share a common ancestor with strains from Asia.
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pone.0142192.g008: Phylogenetic analysis of GB2 genotype from the Philippines.The phylogenetic tree was constructed by adding 98 strains from other countries that were identified by BLASTn search. The Philippine GB2 strains may share a common ancestor with strains from Asia.

Mentions: Two GB2 strains that were detected in February 2013, TEv-13-0101 and TBp-13-0088, were analyzed with 97 GenBank strains with known collection dates obtained from a BLASTn result as of May 22, 2014 and a reference strain known as GB2 strain (BE/90/92: AY751247). GB2 genotype HRSVs in the Philippines might have emerged in 2011 and are closely related with strains detected in several locations in Asia, including China, Hong Kong, Thailand, India, and Japan (Fig 8).


Molecular Characterization of Human Respiratory Syncytial Virus in the Philippines, 2012-2013.

Malasao R, Okamoto M, Chaimongkol N, Imamura T, Tohma K, Dapat I, Dapat C, Suzuki A, Saito M, Saito M, Tamaki R, Pedrera-Rico GA, Aniceto R, Quicho RF, Segubre-Mercado E, Lupisan S, Oshitani H - PLoS ONE (2015)

Phylogenetic analysis of GB2 genotype from the Philippines.The phylogenetic tree was constructed by adding 98 strains from other countries that were identified by BLASTn search. The Philippine GB2 strains may share a common ancestor with strains from Asia.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4635013&req=5

pone.0142192.g008: Phylogenetic analysis of GB2 genotype from the Philippines.The phylogenetic tree was constructed by adding 98 strains from other countries that were identified by BLASTn search. The Philippine GB2 strains may share a common ancestor with strains from Asia.
Mentions: Two GB2 strains that were detected in February 2013, TEv-13-0101 and TBp-13-0088, were analyzed with 97 GenBank strains with known collection dates obtained from a BLASTn result as of May 22, 2014 and a reference strain known as GB2 strain (BE/90/92: AY751247). GB2 genotype HRSVs in the Philippines might have emerged in 2011 and are closely related with strains detected in several locations in Asia, including China, Hong Kong, Thailand, India, and Japan (Fig 8).

Bottom Line: Moreover, in the ON1 genotype, we found positive selection at amino acid position 274 (p<0.05) and massive O- and N-glycosylation in the 2nd HVR of the G gene.However, two sporadic cases of GB2 genotype were found, which might share a common ancestor with other Asian strains.Both genotypes were detected for the first time in the Philippines.

View Article: PubMed Central - PubMed

Affiliation: Tohoku University Graduate School of Medicine, Sendai, Japan.

ABSTRACT
Human respiratory syncytial virus (HRSV) is a major cause of acute lower respiratory tract infections in infants and children worldwide. We performed molecular analysis of HRSV among infants and children with clinical diagnosis of severe pneumonia in four study sites in the Philippines, including Biliran, Leyte, Palawan, and Metro Manila from June 2012 to July 2013. Nasopharyngeal swabs were collected and screened for HRSV using real-time polymerase chain reaction (PCR). Positive samples were tested by conventional PCR and sequenced for the second hypervariable region (2nd HVR) of the G gene. Among a total of 1,505 samples, 423 samples were positive for HRSV (28.1%), of which 305 (72.1%) and 118 (27.9%) were identified as HRSV-A and HRSV-B, respectively. Two genotypes of HRSV-A, NA1 and ON1, were identified during the study period. The novel ON1 genotype with a 72-nucleotide duplication in 2nd HVR of the G gene increased rapidly and finally became the predominant genotype in 2013 with an evolutionary rate higher than the NA1 genotype. Moreover, in the ON1 genotype, we found positive selection at amino acid position 274 (p<0.05) and massive O- and N-glycosylation in the 2nd HVR of the G gene. Among HRSV-B, BA9 was the predominant genotype circulating in the Philippines. However, two sporadic cases of GB2 genotype were found, which might share a common ancestor with other Asian strains. These findings suggest that HRSV is an important cause of severe acute respiratory infection among children in the Philippines and revealed the emergence and subsequent predominance of the ON1 genotype and the sporadic detection of the GB2 genotype. Both genotypes were detected for the first time in the Philippines.

No MeSH data available.


Related in: MedlinePlus