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Effects of Mountain Ultra-Marathon Running on ROS Production and Oxidative Damage by Micro-Invasive Analytic Techniques.

Mrakic-Sposta S, Gussoni M, Moretti S, Pratali L, Giardini G, Tacchini P, Dellanoce C, Tonacci A, Mastorci F, Borghini A, Montorsi M, Vezzoli A - PLoS ONE (2015)

Bottom Line: A significant increase (Post-Race vs Pre) of the ROS production rate (2.20±0.27 vs 1.65±0.22 μmol.min-1), oxidative damage biomarkers (8-OH-dG: 6.32±2.38 vs 4.16±1.25 ng.mg-1 Creatinine and 8-isoPGF2α: 1404.0±518.30 vs 822.51±448.91 pg.mg-1Creatinine), inflammatory state (IL-6-P: 66.42±36.92 vs 1.29±0.54 pg.mL-1 and IL-6-U: 1.33±0.56 vs 0.71±0.17 pg.mL1) and lactate production (+190%), associated with a decrease of both antioxidant capacity (-7%) and renal function (i.e. Creatinine level +76%) was found.The used micro-invasive analytic methods allowed us to perform most of them before, during and immediately after the race directly in the field, by passing the need of storing and transporting samples for further analysis.Considered altogether the investigated variables showed up that exhaustive and prolonged exercise not only promotes the generation of ROS but also induces oxidative stress, transient renal impairment and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioimaging and Molecular Physiology, National Council of Research (CNR), Segrate (Milan), Italy.

ABSTRACT

Purpose: Aiming to gain a detailed insight into the physiological mechanisms involved under extreme conditions, a group of experienced ultra-marathon runners, performing the mountain Tor des Géants® ultra-marathon: 330 km trail-run in Valle d'Aosta, 24000 m of positive and negative elevation changes, was monitored. ROS production rate, antioxidant capacity, oxidative damage and inflammation markers were assessed, adopting micro-invasive analytic techniques.

Methods: Forty-six male athletes (45.04±8.75 yr, 72.6±8.4 kg, 1.76±0.05 m) were tested. Capillary blood and urine were collected before (Pre-), in the middle (Middle-) and immediately after (Post-) Race. Samples were analyzed for: Reactive Oxygen Species (ROS) production by Electron Paramagnetic Resonance; Antioxidant Capacity by Electrochemistry; oxidative damage (8-hydroxy-2-deoxy Guanosine: 8-OH-dG; 8-isoprostane: 8-isoPGF2α) and nitric oxide metabolites by enzymatic assays; inflammatory biomarkers (plasma and urine interleukin-6: IL-6-P and IL-6-U) by enzyme-linked immunosorbent assays (ELISA); Creatinine and Neopterin by HPLC, hematologic (lactate, glucose and hematocrit) and urine parameters by standard analyses.

Results: Twenty-five athletes finished the race, while twenty-one dropped out of it. A significant increase (Post-Race vs Pre) of the ROS production rate (2.20±0.27 vs 1.65±0.22 μmol.min-1), oxidative damage biomarkers (8-OH-dG: 6.32±2.38 vs 4.16±1.25 ng.mg-1 Creatinine and 8-isoPGF2α: 1404.0±518.30 vs 822.51±448.91 pg.mg-1Creatinine), inflammatory state (IL-6-P: 66.42±36.92 vs 1.29±0.54 pg.mL-1 and IL-6-U: 1.33±0.56 vs 0.71±0.17 pg.mL1) and lactate production (+190%), associated with a decrease of both antioxidant capacity (-7%) and renal function (i.e. Creatinine level +76%) was found.

Conclusions: The used micro-invasive analytic methods allowed us to perform most of them before, during and immediately after the race directly in the field, by passing the need of storing and transporting samples for further analysis. Considered altogether the investigated variables showed up that exhaustive and prolonged exercise not only promotes the generation of ROS but also induces oxidative stress, transient renal impairment and inflammation.

No MeSH data available.


Related in: MedlinePlus

Effects of MUM on Oxidative damage biomarkers.Histograms of: A) 8-hydroxy-2-deoxy Guanosine (8-OH-dG, ng.mg-1 creatinine); B) 8-isoprostane (8-iso PGF2α, pg.mg-1 creatinine) concentrations in the FR and NFR groups at Pre and Post-Race. Results are expressed as mean ± SD;* significant differences (P<0.05) compared to Pre-Race.
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pone.0141780.g003: Effects of MUM on Oxidative damage biomarkers.Histograms of: A) 8-hydroxy-2-deoxy Guanosine (8-OH-dG, ng.mg-1 creatinine); B) 8-isoprostane (8-iso PGF2α, pg.mg-1 creatinine) concentrations in the FR and NFR groups at Pre and Post-Race. Results are expressed as mean ± SD;* significant differences (P<0.05) compared to Pre-Race.

Mentions: The trend of the oxidative damage biomarkers is displayed in the histograms of Fig 3A (8-hydroxy-2-deoxy Guanosine) and Fig 3B (8-iso PGF2α) at Pre and Post-Race times. No significant differences were found between the levels calculated for the FR and NRF groups at Pre-Race. As can be observed in the figure, at Post-Race in FR, both markers attain a significantly greater level (P<0.05): 8-OH-dG/creatinine (Post- vs Pre-Race levels 6.32±2.38 vs 4.16±1.25 ng.mg-1) and for 8-iso PGF2α/creatinine (Post- vs Pre-Race levels 1404.0±518.30 vs 822.51±448.91 pg.mg-1).


Effects of Mountain Ultra-Marathon Running on ROS Production and Oxidative Damage by Micro-Invasive Analytic Techniques.

Mrakic-Sposta S, Gussoni M, Moretti S, Pratali L, Giardini G, Tacchini P, Dellanoce C, Tonacci A, Mastorci F, Borghini A, Montorsi M, Vezzoli A - PLoS ONE (2015)

Effects of MUM on Oxidative damage biomarkers.Histograms of: A) 8-hydroxy-2-deoxy Guanosine (8-OH-dG, ng.mg-1 creatinine); B) 8-isoprostane (8-iso PGF2α, pg.mg-1 creatinine) concentrations in the FR and NFR groups at Pre and Post-Race. Results are expressed as mean ± SD;* significant differences (P<0.05) compared to Pre-Race.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4634988&req=5

pone.0141780.g003: Effects of MUM on Oxidative damage biomarkers.Histograms of: A) 8-hydroxy-2-deoxy Guanosine (8-OH-dG, ng.mg-1 creatinine); B) 8-isoprostane (8-iso PGF2α, pg.mg-1 creatinine) concentrations in the FR and NFR groups at Pre and Post-Race. Results are expressed as mean ± SD;* significant differences (P<0.05) compared to Pre-Race.
Mentions: The trend of the oxidative damage biomarkers is displayed in the histograms of Fig 3A (8-hydroxy-2-deoxy Guanosine) and Fig 3B (8-iso PGF2α) at Pre and Post-Race times. No significant differences were found between the levels calculated for the FR and NRF groups at Pre-Race. As can be observed in the figure, at Post-Race in FR, both markers attain a significantly greater level (P<0.05): 8-OH-dG/creatinine (Post- vs Pre-Race levels 6.32±2.38 vs 4.16±1.25 ng.mg-1) and for 8-iso PGF2α/creatinine (Post- vs Pre-Race levels 1404.0±518.30 vs 822.51±448.91 pg.mg-1).

Bottom Line: A significant increase (Post-Race vs Pre) of the ROS production rate (2.20±0.27 vs 1.65±0.22 μmol.min-1), oxidative damage biomarkers (8-OH-dG: 6.32±2.38 vs 4.16±1.25 ng.mg-1 Creatinine and 8-isoPGF2α: 1404.0±518.30 vs 822.51±448.91 pg.mg-1Creatinine), inflammatory state (IL-6-P: 66.42±36.92 vs 1.29±0.54 pg.mL-1 and IL-6-U: 1.33±0.56 vs 0.71±0.17 pg.mL1) and lactate production (+190%), associated with a decrease of both antioxidant capacity (-7%) and renal function (i.e. Creatinine level +76%) was found.The used micro-invasive analytic methods allowed us to perform most of them before, during and immediately after the race directly in the field, by passing the need of storing and transporting samples for further analysis.Considered altogether the investigated variables showed up that exhaustive and prolonged exercise not only promotes the generation of ROS but also induces oxidative stress, transient renal impairment and inflammation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioimaging and Molecular Physiology, National Council of Research (CNR), Segrate (Milan), Italy.

ABSTRACT

Purpose: Aiming to gain a detailed insight into the physiological mechanisms involved under extreme conditions, a group of experienced ultra-marathon runners, performing the mountain Tor des Géants® ultra-marathon: 330 km trail-run in Valle d'Aosta, 24000 m of positive and negative elevation changes, was monitored. ROS production rate, antioxidant capacity, oxidative damage and inflammation markers were assessed, adopting micro-invasive analytic techniques.

Methods: Forty-six male athletes (45.04±8.75 yr, 72.6±8.4 kg, 1.76±0.05 m) were tested. Capillary blood and urine were collected before (Pre-), in the middle (Middle-) and immediately after (Post-) Race. Samples were analyzed for: Reactive Oxygen Species (ROS) production by Electron Paramagnetic Resonance; Antioxidant Capacity by Electrochemistry; oxidative damage (8-hydroxy-2-deoxy Guanosine: 8-OH-dG; 8-isoprostane: 8-isoPGF2α) and nitric oxide metabolites by enzymatic assays; inflammatory biomarkers (plasma and urine interleukin-6: IL-6-P and IL-6-U) by enzyme-linked immunosorbent assays (ELISA); Creatinine and Neopterin by HPLC, hematologic (lactate, glucose and hematocrit) and urine parameters by standard analyses.

Results: Twenty-five athletes finished the race, while twenty-one dropped out of it. A significant increase (Post-Race vs Pre) of the ROS production rate (2.20±0.27 vs 1.65±0.22 μmol.min-1), oxidative damage biomarkers (8-OH-dG: 6.32±2.38 vs 4.16±1.25 ng.mg-1 Creatinine and 8-isoPGF2α: 1404.0±518.30 vs 822.51±448.91 pg.mg-1Creatinine), inflammatory state (IL-6-P: 66.42±36.92 vs 1.29±0.54 pg.mL-1 and IL-6-U: 1.33±0.56 vs 0.71±0.17 pg.mL1) and lactate production (+190%), associated with a decrease of both antioxidant capacity (-7%) and renal function (i.e. Creatinine level +76%) was found.

Conclusions: The used micro-invasive analytic methods allowed us to perform most of them before, during and immediately after the race directly in the field, by passing the need of storing and transporting samples for further analysis. Considered altogether the investigated variables showed up that exhaustive and prolonged exercise not only promotes the generation of ROS but also induces oxidative stress, transient renal impairment and inflammation.

No MeSH data available.


Related in: MedlinePlus