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Thioredoxin-2 Modulates Neuronal Programmed Cell Death in the Embryonic Chick Spinal Cord in Basal and Target-Deprived Conditions.

Pirson M, Debrulle S, Clippe A, Clotman F, Knoops B - PLoS ONE (2015)

Bottom Line: Thioredoxin-2 (Trx2) is a mitochondrial protein using a dithiol active site to reduce protein disulfides.Using in ovo electroporation, we go on to highlight a cytoprotective effect of Trx2 on the programmed cell death (PCD) of neurons during spinal cord development and in a novel cultured spinal cord explant model.These findings suggest an implication of Trx2 in the modulation of developmental PCD of neurons during embryonic development of the spinal cord, possibly through redox regulation mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Group of Animal Molecular and Cellular Biology, Institut des Sciences de la Vie (ISV), Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.

ABSTRACT
Thioredoxin-2 (Trx2) is a mitochondrial protein using a dithiol active site to reduce protein disulfides. In addition to the cytoprotective function of this enzyme, several studies have highlighted the implication of Trx2 in cellular signaling events. In particular, growing evidence points to such roles of redox enzymes in developmental processes taking place in the central nervous system. Here, we investigate the potential implication of Trx2 in embryonic development of chick spinal cord. To this end, we first studied the distribution of the enzyme in this tissue and report strong expression of Trx2 in chick embryo post-mitotic neurons at E4.5 and in motor neurons at E6.5. Using in ovo electroporation, we go on to highlight a cytoprotective effect of Trx2 on the programmed cell death (PCD) of neurons during spinal cord development and in a novel cultured spinal cord explant model. These findings suggest an implication of Trx2 in the modulation of developmental PCD of neurons during embryonic development of the spinal cord, possibly through redox regulation mechanisms.

No MeSH data available.


Related in: MedlinePlus

Trx2 colocalizes with NeuN-positive cells at E4.5 and Isl1/2 positive cells at E6.5.Colocalization of Trx2 with NeuN at E4.5 (A-C) and with Isl1/2 at E6.5 (D-F) in the embryonic chick spinal cord. Scale bar = 50 μm.
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pone.0142280.g003: Trx2 colocalizes with NeuN-positive cells at E4.5 and Isl1/2 positive cells at E6.5.Colocalization of Trx2 with NeuN at E4.5 (A-C) and with Isl1/2 at E6.5 (D-F) in the embryonic chick spinal cord. Scale bar = 50 μm.

Mentions: To uncover putative expression patterns of Trx2 during chick spinal cord development, immunofluorescence assays using Trx2 antibody were carried out at E4.5 and E6.5 (Fig 3). Anti-Trx2 antibody produced signal in all of the spinal cord but more prominently in lateral territories of the E4.5 spinal cord and in ventro-lateral territories at E6.5 (Fig 3A and 3D, respectively). This lateral staining colocalized with post-mitotic neuron marker NeuN at E4.5 (Fig 3A–3C) and with motor neuron marker Isl1/2 at E6.5 (Fig 3D–3F). It is of note that at both stages, Trx2 labeling was not exclusive to these cell populations. Indeed, Trx2 immunoreactivity was also visible in certain Isl1/2-negative cells located in the ventral spinal cord. Moreover, though Trx2 antibody showed intense staining in certain Isl1/2-positive cells, other Isl1/2-positive cells displayed more moderate immunoreactivity.


Thioredoxin-2 Modulates Neuronal Programmed Cell Death in the Embryonic Chick Spinal Cord in Basal and Target-Deprived Conditions.

Pirson M, Debrulle S, Clippe A, Clotman F, Knoops B - PLoS ONE (2015)

Trx2 colocalizes with NeuN-positive cells at E4.5 and Isl1/2 positive cells at E6.5.Colocalization of Trx2 with NeuN at E4.5 (A-C) and with Isl1/2 at E6.5 (D-F) in the embryonic chick spinal cord. Scale bar = 50 μm.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4634972&req=5

pone.0142280.g003: Trx2 colocalizes with NeuN-positive cells at E4.5 and Isl1/2 positive cells at E6.5.Colocalization of Trx2 with NeuN at E4.5 (A-C) and with Isl1/2 at E6.5 (D-F) in the embryonic chick spinal cord. Scale bar = 50 μm.
Mentions: To uncover putative expression patterns of Trx2 during chick spinal cord development, immunofluorescence assays using Trx2 antibody were carried out at E4.5 and E6.5 (Fig 3). Anti-Trx2 antibody produced signal in all of the spinal cord but more prominently in lateral territories of the E4.5 spinal cord and in ventro-lateral territories at E6.5 (Fig 3A and 3D, respectively). This lateral staining colocalized with post-mitotic neuron marker NeuN at E4.5 (Fig 3A–3C) and with motor neuron marker Isl1/2 at E6.5 (Fig 3D–3F). It is of note that at both stages, Trx2 labeling was not exclusive to these cell populations. Indeed, Trx2 immunoreactivity was also visible in certain Isl1/2-negative cells located in the ventral spinal cord. Moreover, though Trx2 antibody showed intense staining in certain Isl1/2-positive cells, other Isl1/2-positive cells displayed more moderate immunoreactivity.

Bottom Line: Thioredoxin-2 (Trx2) is a mitochondrial protein using a dithiol active site to reduce protein disulfides.Using in ovo electroporation, we go on to highlight a cytoprotective effect of Trx2 on the programmed cell death (PCD) of neurons during spinal cord development and in a novel cultured spinal cord explant model.These findings suggest an implication of Trx2 in the modulation of developmental PCD of neurons during embryonic development of the spinal cord, possibly through redox regulation mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Group of Animal Molecular and Cellular Biology, Institut des Sciences de la Vie (ISV), Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.

ABSTRACT
Thioredoxin-2 (Trx2) is a mitochondrial protein using a dithiol active site to reduce protein disulfides. In addition to the cytoprotective function of this enzyme, several studies have highlighted the implication of Trx2 in cellular signaling events. In particular, growing evidence points to such roles of redox enzymes in developmental processes taking place in the central nervous system. Here, we investigate the potential implication of Trx2 in embryonic development of chick spinal cord. To this end, we first studied the distribution of the enzyme in this tissue and report strong expression of Trx2 in chick embryo post-mitotic neurons at E4.5 and in motor neurons at E6.5. Using in ovo electroporation, we go on to highlight a cytoprotective effect of Trx2 on the programmed cell death (PCD) of neurons during spinal cord development and in a novel cultured spinal cord explant model. These findings suggest an implication of Trx2 in the modulation of developmental PCD of neurons during embryonic development of the spinal cord, possibly through redox regulation mechanisms.

No MeSH data available.


Related in: MedlinePlus