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Effect of Dietary Docosahexaenoic Acid Supplementation on the Participation of Vasodilator Factors in Aorta from Orchidectomized Rats.

Villalpando DM, Navarro R, Del Campo L, Largo C, Muñoz D, Tabernero M, Baeza R, Otero C, García HS, Ferrer M - PLoS ONE (2015)

Bottom Line: The results showed that orchidectomy negatively affected the lipid profile, increased the production of prostanoids and reactive oxygen species (ROS), and decreased NO production and the antioxidant capacity, as well as the participation of hyperpolarizing mechanisms in the vasodilator responses.The DHA-supplemented diet of the orchidectomized rats decreased the release of prostanoids and ROS, while increasing NO production and the antioxidant capacity, and it also improved the lipid profile.Additionally, it restored the participation of hyperpolarizing mechanisms by activating potassium.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain.

ABSTRACT
Benefits of n-3 polyunsaturated fatty acids (PUFAs) against cardiovascular diseases have been reported. Vascular tone regulation is largely mediated by endothelial factors whose release is modulated by sex hormones. Since the incidence of cardiovascular pathologies has been correlated with decreased levels of sex hormones, the aim of this study was to analyze whether a diet supplemented with the specific PUFA docosahexaenoic acid (DHA) could prevent vascular changes induced by an impaired gonadal function. For this purpose, control and orchidectomized rats were fed with a standard diet supplemented with 5% (w/w) sunflower oil or with 3% (w/w) sunflower oil plus 2% (w/w) DHA. The lipid profile, the blood pressure, the production of prostanoids and nitric oxide (NO), and the redox status of biological samples from control and orchidectomized rats, fed control or DHA-supplemented diet, were analyzed. The vasodilator response and the contribution of NO, prostanoids and hyperpolarizing mechanisms were also studied. The results showed that orchidectomy negatively affected the lipid profile, increased the production of prostanoids and reactive oxygen species (ROS), and decreased NO production and the antioxidant capacity, as well as the participation of hyperpolarizing mechanisms in the vasodilator responses. The DHA-supplemented diet of the orchidectomized rats decreased the release of prostanoids and ROS, while increasing NO production and the antioxidant capacity, and it also improved the lipid profile. Additionally, it restored the participation of hyperpolarizing mechanisms by activating potassium. Since the modifications induced by the DHA-supplemented diet were observed in the orchidectomized, but not in the healthy group, DHA seems to exert cardioprotective effects in physiopathological situations in which vascular dysfunction exists.

No MeSH data available.


Related in: MedlinePlus

Effect of orchidectomy and the DHA-supplemented diet in the H2O2 content in rat serum and aorta.Relative levels of H2O2 in serum and aortic rings from control (C) and orchidectomized (ORX) rats fed with a control or with a DHA-supplemented diet. Values are means ± SEMs. Number of animals, n = 4. *P < 0.01 compared with control animals; +P < 0.01 compared with ORX rats control diet.
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pone.0142039.g003: Effect of orchidectomy and the DHA-supplemented diet in the H2O2 content in rat serum and aorta.Relative levels of H2O2 in serum and aortic rings from control (C) and orchidectomized (ORX) rats fed with a control or with a DHA-supplemented diet. Values are means ± SEMs. Number of animals, n = 4. *P < 0.01 compared with control animals; +P < 0.01 compared with ORX rats control diet.

Mentions: The levels of hydrogen peroxide, in serum samples and aortic wall, were increased in orchidectomized rats, which were returned to control level when orchidectomized rats fed with the DHA-supplemented diet. The DHA-diet to control animals did not modify hydrogen peroxide levels (Fig 3).


Effect of Dietary Docosahexaenoic Acid Supplementation on the Participation of Vasodilator Factors in Aorta from Orchidectomized Rats.

Villalpando DM, Navarro R, Del Campo L, Largo C, Muñoz D, Tabernero M, Baeza R, Otero C, García HS, Ferrer M - PLoS ONE (2015)

Effect of orchidectomy and the DHA-supplemented diet in the H2O2 content in rat serum and aorta.Relative levels of H2O2 in serum and aortic rings from control (C) and orchidectomized (ORX) rats fed with a control or with a DHA-supplemented diet. Values are means ± SEMs. Number of animals, n = 4. *P < 0.01 compared with control animals; +P < 0.01 compared with ORX rats control diet.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4634962&req=5

pone.0142039.g003: Effect of orchidectomy and the DHA-supplemented diet in the H2O2 content in rat serum and aorta.Relative levels of H2O2 in serum and aortic rings from control (C) and orchidectomized (ORX) rats fed with a control or with a DHA-supplemented diet. Values are means ± SEMs. Number of animals, n = 4. *P < 0.01 compared with control animals; +P < 0.01 compared with ORX rats control diet.
Mentions: The levels of hydrogen peroxide, in serum samples and aortic wall, were increased in orchidectomized rats, which were returned to control level when orchidectomized rats fed with the DHA-supplemented diet. The DHA-diet to control animals did not modify hydrogen peroxide levels (Fig 3).

Bottom Line: The results showed that orchidectomy negatively affected the lipid profile, increased the production of prostanoids and reactive oxygen species (ROS), and decreased NO production and the antioxidant capacity, as well as the participation of hyperpolarizing mechanisms in the vasodilator responses.The DHA-supplemented diet of the orchidectomized rats decreased the release of prostanoids and ROS, while increasing NO production and the antioxidant capacity, and it also improved the lipid profile.Additionally, it restored the participation of hyperpolarizing mechanisms by activating potassium.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain.

ABSTRACT
Benefits of n-3 polyunsaturated fatty acids (PUFAs) against cardiovascular diseases have been reported. Vascular tone regulation is largely mediated by endothelial factors whose release is modulated by sex hormones. Since the incidence of cardiovascular pathologies has been correlated with decreased levels of sex hormones, the aim of this study was to analyze whether a diet supplemented with the specific PUFA docosahexaenoic acid (DHA) could prevent vascular changes induced by an impaired gonadal function. For this purpose, control and orchidectomized rats were fed with a standard diet supplemented with 5% (w/w) sunflower oil or with 3% (w/w) sunflower oil plus 2% (w/w) DHA. The lipid profile, the blood pressure, the production of prostanoids and nitric oxide (NO), and the redox status of biological samples from control and orchidectomized rats, fed control or DHA-supplemented diet, were analyzed. The vasodilator response and the contribution of NO, prostanoids and hyperpolarizing mechanisms were also studied. The results showed that orchidectomy negatively affected the lipid profile, increased the production of prostanoids and reactive oxygen species (ROS), and decreased NO production and the antioxidant capacity, as well as the participation of hyperpolarizing mechanisms in the vasodilator responses. The DHA-supplemented diet of the orchidectomized rats decreased the release of prostanoids and ROS, while increasing NO production and the antioxidant capacity, and it also improved the lipid profile. Additionally, it restored the participation of hyperpolarizing mechanisms by activating potassium. Since the modifications induced by the DHA-supplemented diet were observed in the orchidectomized, but not in the healthy group, DHA seems to exert cardioprotective effects in physiopathological situations in which vascular dysfunction exists.

No MeSH data available.


Related in: MedlinePlus