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The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation.

Peng X, Teng L, Yan X, Zhao M, Shen S - BMC Genomics (2015)

Bottom Line: For screening of differentially expressed genes, the expression level of every transcript in each sample was calculated by quantifying the number of Illumina reads.Finally, real time PCR was used to verify the DEG results of the RNA-seq and the proteomics data.Total of 5800 unigenes and 38 proteins were affected, including the repressed expression of photosynthesis and the enhanced expression in signal transduction, stress defense pathway as well as secondary metabolism.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, China. pengxianjun@ibcas.ac.cn.

ABSTRACT

Background: Most studies on the paper mulberry are mainly focused on the medicated and pharmacology, fiber quality, leaves feed development, little is known about its mechanism of adaptability to abiotic stress. Physiological measurement, transcriptomics and proteomic analysis were employed to understand its response to cold stress in this study.

Methods: The second to fourth fully expanded leaves from up to down were harvested at different stress time points forthe transmission electron microscope (TEM) observation. Physiological characteristics measurement included the relative electrolyte leakage (REL), SOD activity assay, soluble sugar content, and Chlorophyll fluorescence parameter measurement. For screening of differentially expressed genes, the expression level of every transcript in each sample was calculated by quantifying the number of Illumina reads. To identify the differentially expressed protein, leaves of plants under 0, 6, 12, 24, 48 and 72 h cold stress wereharvested for proteomic analysis. Finally, real time PCR was used to verify the DEG results of the RNA-seq and the proteomics data.

Results: Results showed that at the beginning of cold stress, respiratory metabolism was decreased and the transportation and hydrolysis of photosynthetic products was inhibited, leading to an accumulation of starch in the chloroplasts. Total of 5800 unigenes and 38 proteins were affected, including the repressed expression of photosynthesis and the enhanced expression in signal transduction, stress defense pathway as well as secondary metabolism. Although the transcriptional level of a large number of genes has been restored after 12 h, sustained cold stress brought more serious injury to the leaf cells, including the sharp rise of the relative electrolyte leakage, the declined Fv/Fm value, swelled chloroplast and the disintegrated membrane system.

Conclusion: The starch accumulation and the photoinhibition might be the main adaptive mechanism of the paper mulberry responded to cold stress. Most of important, enhancing the transport and hydrolysis of photosynthetic products could be the potential targets for improving the cold tolerance of the paper mulberry.

No MeSH data available.


Related in: MedlinePlus

The number of DEGs related to phytohormones signaling pathway and transcriptional factor. a Statistics of DEGs involved in the phytohormones signaling pathway. b No. of differentially expressed TFs in each family
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Fig5: The number of DEGs related to phytohormones signaling pathway and transcriptional factor. a Statistics of DEGs involved in the phytohormones signaling pathway. b No. of differentially expressed TFs in each family

Mentions: The signal transduction pathway plays a pivotal role in the response to the stress of low temperatures and cold stress-induced second messenger can be decoded by different pathways, including Ca2+, ROS, Receptor-like protein kinases and other signal pathways (Additional files 2, 3 and 4). Phytohormones, such as ABA (abscisic acid), auxin, BR (brassinosteroid ), CK (cytokinin), ETH (ethylene), GA (gibberellic acid), JA (jasmonates) and SA (salicylic acid) are related to the cold responses positively or negatively. They have been shown to play an important role in mediating ROS and other cold stress signals. In our study, there were 162, 50, 42, 34, 89, 77, 263 and 283 DEGs related to ABA, auxin, BR, CK, ETH, GA, JA and SA mediated signaling pathway in the GO annotation result, respectively (Fig. 5a).Fig. 5


The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation.

Peng X, Teng L, Yan X, Zhao M, Shen S - BMC Genomics (2015)

The number of DEGs related to phytohormones signaling pathway and transcriptional factor. a Statistics of DEGs involved in the phytohormones signaling pathway. b No. of differentially expressed TFs in each family
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4634900&req=5

Fig5: The number of DEGs related to phytohormones signaling pathway and transcriptional factor. a Statistics of DEGs involved in the phytohormones signaling pathway. b No. of differentially expressed TFs in each family
Mentions: The signal transduction pathway plays a pivotal role in the response to the stress of low temperatures and cold stress-induced second messenger can be decoded by different pathways, including Ca2+, ROS, Receptor-like protein kinases and other signal pathways (Additional files 2, 3 and 4). Phytohormones, such as ABA (abscisic acid), auxin, BR (brassinosteroid ), CK (cytokinin), ETH (ethylene), GA (gibberellic acid), JA (jasmonates) and SA (salicylic acid) are related to the cold responses positively or negatively. They have been shown to play an important role in mediating ROS and other cold stress signals. In our study, there were 162, 50, 42, 34, 89, 77, 263 and 283 DEGs related to ABA, auxin, BR, CK, ETH, GA, JA and SA mediated signaling pathway in the GO annotation result, respectively (Fig. 5a).Fig. 5

Bottom Line: For screening of differentially expressed genes, the expression level of every transcript in each sample was calculated by quantifying the number of Illumina reads.Finally, real time PCR was used to verify the DEG results of the RNA-seq and the proteomics data.Total of 5800 unigenes and 38 proteins were affected, including the repressed expression of photosynthesis and the enhanced expression in signal transduction, stress defense pathway as well as secondary metabolism.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, China. pengxianjun@ibcas.ac.cn.

ABSTRACT

Background: Most studies on the paper mulberry are mainly focused on the medicated and pharmacology, fiber quality, leaves feed development, little is known about its mechanism of adaptability to abiotic stress. Physiological measurement, transcriptomics and proteomic analysis were employed to understand its response to cold stress in this study.

Methods: The second to fourth fully expanded leaves from up to down were harvested at different stress time points forthe transmission electron microscope (TEM) observation. Physiological characteristics measurement included the relative electrolyte leakage (REL), SOD activity assay, soluble sugar content, and Chlorophyll fluorescence parameter measurement. For screening of differentially expressed genes, the expression level of every transcript in each sample was calculated by quantifying the number of Illumina reads. To identify the differentially expressed protein, leaves of plants under 0, 6, 12, 24, 48 and 72 h cold stress wereharvested for proteomic analysis. Finally, real time PCR was used to verify the DEG results of the RNA-seq and the proteomics data.

Results: Results showed that at the beginning of cold stress, respiratory metabolism was decreased and the transportation and hydrolysis of photosynthetic products was inhibited, leading to an accumulation of starch in the chloroplasts. Total of 5800 unigenes and 38 proteins were affected, including the repressed expression of photosynthesis and the enhanced expression in signal transduction, stress defense pathway as well as secondary metabolism. Although the transcriptional level of a large number of genes has been restored after 12 h, sustained cold stress brought more serious injury to the leaf cells, including the sharp rise of the relative electrolyte leakage, the declined Fv/Fm value, swelled chloroplast and the disintegrated membrane system.

Conclusion: The starch accumulation and the photoinhibition might be the main adaptive mechanism of the paper mulberry responded to cold stress. Most of important, enhancing the transport and hydrolysis of photosynthetic products could be the potential targets for improving the cold tolerance of the paper mulberry.

No MeSH data available.


Related in: MedlinePlus