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Molecular Characterization and Differential Expression of an Olfactory Receptor Gene Family in the White-Backed Planthopper Sogatella furcifera Based on Transcriptome Analysis.

He M, Zhang YN, He P - PLoS ONE (2015)

Bottom Line: Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates.Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species.Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, P. R. China.

ABSTRACT
The white-backed planthopper, Sogatella furcifera, a notorious rice pest in Asia, employs host plant volatiles as cues for host location. In insects, odor detection is mediated by two types of olfactory receptors: odorant receptors (ORs) and ionotropic receptors (IRs). In this study, we identified 63 SfurORs and 14 SfurIRs in S. furcifera based on sequences obtained from the head transcriptome and bioinformatics analysis. The motif-pattern of 130 hemiptera ORs indicated an apparent differentiation in this order. Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates. Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species. Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree of putative S. furcifera IRs, Drosophila melanogaster iGluRs and IRs, and other insect IRs.SfurIRs are highlighted in red letters. Bmor, Bombyx mori; Cpom, Cydia pomonella; Dmel, D. melanogaster; Dple, Danaus plexippus; Harm, Helicoverpa armigera; Msex, Manduca sexta; Slit, Spodoptera littoralis; Snon, Sesamia nonagrioides; Ago, A. gossypii.
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pone.0140605.g005: Phylogenetic tree of putative S. furcifera IRs, Drosophila melanogaster iGluRs and IRs, and other insect IRs.SfurIRs are highlighted in red letters. Bmor, Bombyx mori; Cpom, Cydia pomonella; Dmel, D. melanogaster; Dple, Danaus plexippus; Harm, Helicoverpa armigera; Msex, Manduca sexta; Slit, Spodoptera littoralis; Snon, Sesamia nonagrioides; Ago, A. gossypii.

Mentions: To distinguish putative OR or IR functions, we also constructed two phylogenetic trees using 57 ORs (>150 aa), 7 IRs, and 3 iGluRs (>120 aa) from S. furcifera, as well as known hemipteran ORs (Fig 4) and other insect IRs (Fig 5). In the OR tree, the co-receptor ortholog of S. furcifera SfurORco was easily assigned because it shared high similarity with the conserved hemipteran co-receptors. Other ORs were assigned to various clades, which indicated their distinct functions. Most of the other SfurORs had orthologous genes, such as SfurOR21/28/29/ApOR29, SfurOR59/ApOR5/AgoOR5, SfurOR3/RproOR-trT1H999, SfurOR18/56/RproOR-trT1H8H6, and SfurOR20/RproOR-trT1H805. In the IR tree, all 7 SfurIRs and 3 SfuriGluRs were assigned to known insect IR clades, i.e., SfurIR3 (IR64a clade), SfurIR4 (IR8a clade), SfuriGluR1/2/3 (ionotropic glutamate receptor clade), SfurIR9 (IR25a clade), SfurIR10 (unidentified clade), SfurIR11 (IR40a clade), SfurIR14 (IR76b clade), and SfurIR15 (IR93a clade).


Molecular Characterization and Differential Expression of an Olfactory Receptor Gene Family in the White-Backed Planthopper Sogatella furcifera Based on Transcriptome Analysis.

He M, Zhang YN, He P - PLoS ONE (2015)

Phylogenetic tree of putative S. furcifera IRs, Drosophila melanogaster iGluRs and IRs, and other insect IRs.SfurIRs are highlighted in red letters. Bmor, Bombyx mori; Cpom, Cydia pomonella; Dmel, D. melanogaster; Dple, Danaus plexippus; Harm, Helicoverpa armigera; Msex, Manduca sexta; Slit, Spodoptera littoralis; Snon, Sesamia nonagrioides; Ago, A. gossypii.
© Copyright Policy
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4634861&req=5

pone.0140605.g005: Phylogenetic tree of putative S. furcifera IRs, Drosophila melanogaster iGluRs and IRs, and other insect IRs.SfurIRs are highlighted in red letters. Bmor, Bombyx mori; Cpom, Cydia pomonella; Dmel, D. melanogaster; Dple, Danaus plexippus; Harm, Helicoverpa armigera; Msex, Manduca sexta; Slit, Spodoptera littoralis; Snon, Sesamia nonagrioides; Ago, A. gossypii.
Mentions: To distinguish putative OR or IR functions, we also constructed two phylogenetic trees using 57 ORs (>150 aa), 7 IRs, and 3 iGluRs (>120 aa) from S. furcifera, as well as known hemipteran ORs (Fig 4) and other insect IRs (Fig 5). In the OR tree, the co-receptor ortholog of S. furcifera SfurORco was easily assigned because it shared high similarity with the conserved hemipteran co-receptors. Other ORs were assigned to various clades, which indicated their distinct functions. Most of the other SfurORs had orthologous genes, such as SfurOR21/28/29/ApOR29, SfurOR59/ApOR5/AgoOR5, SfurOR3/RproOR-trT1H999, SfurOR18/56/RproOR-trT1H8H6, and SfurOR20/RproOR-trT1H805. In the IR tree, all 7 SfurIRs and 3 SfuriGluRs were assigned to known insect IR clades, i.e., SfurIR3 (IR64a clade), SfurIR4 (IR8a clade), SfuriGluR1/2/3 (ionotropic glutamate receptor clade), SfurIR9 (IR25a clade), SfurIR10 (unidentified clade), SfurIR11 (IR40a clade), SfurIR14 (IR76b clade), and SfurIR15 (IR93a clade).

Bottom Line: Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates.Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species.Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, P. R. China.

ABSTRACT
The white-backed planthopper, Sogatella furcifera, a notorious rice pest in Asia, employs host plant volatiles as cues for host location. In insects, odor detection is mediated by two types of olfactory receptors: odorant receptors (ORs) and ionotropic receptors (IRs). In this study, we identified 63 SfurORs and 14 SfurIRs in S. furcifera based on sequences obtained from the head transcriptome and bioinformatics analysis. The motif-pattern of 130 hemiptera ORs indicated an apparent differentiation in this order. Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates. Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species. Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

No MeSH data available.


Related in: MedlinePlus