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Molecular Characterization and Differential Expression of an Olfactory Receptor Gene Family in the White-Backed Planthopper Sogatella furcifera Based on Transcriptome Analysis.

He M, Zhang YN, He P - PLoS ONE (2015)

Bottom Line: Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates.Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species.Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, P. R. China.

ABSTRACT
The white-backed planthopper, Sogatella furcifera, a notorious rice pest in Asia, employs host plant volatiles as cues for host location. In insects, odor detection is mediated by two types of olfactory receptors: odorant receptors (ORs) and ionotropic receptors (IRs). In this study, we identified 63 SfurORs and 14 SfurIRs in S. furcifera based on sequences obtained from the head transcriptome and bioinformatics analysis. The motif-pattern of 130 hemiptera ORs indicated an apparent differentiation in this order. Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates. Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species. Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

No MeSH data available.


Related in: MedlinePlus

Annotation summaries for S. furcifera head unigenes.(A) Species distribution of unigenes with the best hit annotation terms in the NR database. (B) Gene ontology (GO) classifications of S. furcifera unigenes.
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pone.0140605.g002: Annotation summaries for S. furcifera head unigenes.(A) Species distribution of unigenes with the best hit annotation terms in the NR database. (B) Gene ontology (GO) classifications of S. furcifera unigenes.

Mentions: BLASTx homology searches of all 43,712 unigenes showed that 14,430 (33.1%) had homologous genes in the non-redundant (NR) protein database with a cut-off E-value of 10−5. The best match percentage (14.3%) was with Tribolium castaneum sequences, followed by sequences from Acyrthosiphon pisum (13.2%), Pediculus humanus (8.6%), Nasonia vitripennis (4.7%), and Megachile rotundata (4.6%) (Fig 2A).


Molecular Characterization and Differential Expression of an Olfactory Receptor Gene Family in the White-Backed Planthopper Sogatella furcifera Based on Transcriptome Analysis.

He M, Zhang YN, He P - PLoS ONE (2015)

Annotation summaries for S. furcifera head unigenes.(A) Species distribution of unigenes with the best hit annotation terms in the NR database. (B) Gene ontology (GO) classifications of S. furcifera unigenes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4634861&req=5

pone.0140605.g002: Annotation summaries for S. furcifera head unigenes.(A) Species distribution of unigenes with the best hit annotation terms in the NR database. (B) Gene ontology (GO) classifications of S. furcifera unigenes.
Mentions: BLASTx homology searches of all 43,712 unigenes showed that 14,430 (33.1%) had homologous genes in the non-redundant (NR) protein database with a cut-off E-value of 10−5. The best match percentage (14.3%) was with Tribolium castaneum sequences, followed by sequences from Acyrthosiphon pisum (13.2%), Pediculus humanus (8.6%), Nasonia vitripennis (4.7%), and Megachile rotundata (4.6%) (Fig 2A).

Bottom Line: Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates.Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species.Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, P. R. China.

ABSTRACT
The white-backed planthopper, Sogatella furcifera, a notorious rice pest in Asia, employs host plant volatiles as cues for host location. In insects, odor detection is mediated by two types of olfactory receptors: odorant receptors (ORs) and ionotropic receptors (IRs). In this study, we identified 63 SfurORs and 14 SfurIRs in S. furcifera based on sequences obtained from the head transcriptome and bioinformatics analysis. The motif-pattern of 130 hemiptera ORs indicated an apparent differentiation in this order. Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates. Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species. Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

No MeSH data available.


Related in: MedlinePlus