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Molecular Characterization and Differential Expression of an Olfactory Receptor Gene Family in the White-Backed Planthopper Sogatella furcifera Based on Transcriptome Analysis.

He M, Zhang YN, He P - PLoS ONE (2015)

Bottom Line: Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates.Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species.Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, P. R. China.

ABSTRACT
The white-backed planthopper, Sogatella furcifera, a notorious rice pest in Asia, employs host plant volatiles as cues for host location. In insects, odor detection is mediated by two types of olfactory receptors: odorant receptors (ORs) and ionotropic receptors (IRs). In this study, we identified 63 SfurORs and 14 SfurIRs in S. furcifera based on sequences obtained from the head transcriptome and bioinformatics analysis. The motif-pattern of 130 hemiptera ORs indicated an apparent differentiation in this order. Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates. Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species. Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

No MeSH data available.


Related in: MedlinePlus

Distribution of transcripts and unigene length in the S. furcifera transcriptome assembly.
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pone.0140605.g001: Distribution of transcripts and unigene length in the S. furcifera transcriptome assembly.

Mentions: The S. furcifera head transcriptome was sequenced using the Illumina HiSeq™ 2000 platform and assembled with Trinity (v2012-10-05) (Table 1 and Fig 1). In total, about 163 million reads were obtained. After filtering, 142 million clean reads were generated, which comprised 14.2 gigabases (Gb), with a longest length of 28,290 nt and a median length of 456 nt. These reads were assembled into 89,810 transcripts and 43,712 unigenes, with N50 lengths of 3,014 and 2,217 nt, respectively (Table 1). In addition, the unigenes with a sequence length >1000 nt accounted for 29.63% of the transcriptome assembly (Fig 1). The transcriptome raw reads have been deposited with the NCBI SRA database (accession number: SRR2068690).


Molecular Characterization and Differential Expression of an Olfactory Receptor Gene Family in the White-Backed Planthopper Sogatella furcifera Based on Transcriptome Analysis.

He M, Zhang YN, He P - PLoS ONE (2015)

Distribution of transcripts and unigene length in the S. furcifera transcriptome assembly.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4634861&req=5

pone.0140605.g001: Distribution of transcripts and unigene length in the S. furcifera transcriptome assembly.
Mentions: The S. furcifera head transcriptome was sequenced using the Illumina HiSeq™ 2000 platform and assembled with Trinity (v2012-10-05) (Table 1 and Fig 1). In total, about 163 million reads were obtained. After filtering, 142 million clean reads were generated, which comprised 14.2 gigabases (Gb), with a longest length of 28,290 nt and a median length of 456 nt. These reads were assembled into 89,810 transcripts and 43,712 unigenes, with N50 lengths of 3,014 and 2,217 nt, respectively (Table 1). In addition, the unigenes with a sequence length >1000 nt accounted for 29.63% of the transcriptome assembly (Fig 1). The transcriptome raw reads have been deposited with the NCBI SRA database (accession number: SRR2068690).

Bottom Line: Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates.Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species.Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, P. R. China.

ABSTRACT
The white-backed planthopper, Sogatella furcifera, a notorious rice pest in Asia, employs host plant volatiles as cues for host location. In insects, odor detection is mediated by two types of olfactory receptors: odorant receptors (ORs) and ionotropic receptors (IRs). In this study, we identified 63 SfurORs and 14 SfurIRs in S. furcifera based on sequences obtained from the head transcriptome and bioinformatics analysis. The motif-pattern of 130 hemiptera ORs indicated an apparent differentiation in this order. Phylogenetic trees of the ORs and IRs were constructed using neighbor-joining estimates. Most of the ORs had orthologous genes, but a specific OR clade was identified in S. furcifera, which suggests that these ORs may have specific olfactory functions in this species. Our results provide a basis for further investigations of how S. furcifera coordinates its olfactory receptor genes with its plant hosts, thereby providing a foundation for novel pest management approaches based on these genes.

No MeSH data available.


Related in: MedlinePlus