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Systemic Resistance to Powdery Mildew in Brassica napus (AACC) and Raphanus alboglabra (RRCC) by Trichoderma harzianum TH12.

Alkooranee JT, Yin Y, Aledan TR, Jiang Y, Lu G, Wu J, Li M - PLoS ONE (2015)

Bottom Line: In addition, the gene expression of six plant defense-related genes, namely, PR-1, PR-2 (a marker for SA signaling), PR-3, PDF 1.2 (a marker for JA/ET signaling), CHI620, and CHI570, for both genotypes were analyzed in the leaves of B. napus and R. alboglabra after treatment with TH12 or CF and compared with the non-treated ones.The qRT-PCR results showed that the PR-1 and PR-2 expression levels increased in E. cruciferarum-infected leaves, but decreased in the TH12-treated leaves compared with leaves treated with CF.The expression levels of PR-3 and PDF1.2 decreased in plants infected by E. cruciferarum.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT
Trichoderma harzianum TH12 is a microbial pesticide for certain rapeseed diseases. The mechanism of systemic resistance induced by TH12 or its cell-free culture filtrate (CF) in Brassica napus (AACC) and Raphanus alboglabra (RRCC) to powdery mildew disease caused by ascomycete Erysiphe cruciferarum was investigated. In this study, we conducted the first large-scale global study on the cellular and molecular aspects of B. napus and R. alboglabra infected with E. cruciferarum. The histological study showed the resistance of R. alboglabra to powdery mildew disease. The growth of fungal colonies was not observed on R. alboglabra leaves at 1, 2, 4, 6, 8, and 10 days post-inoculation (dpi), whereas this was clearly observed on B. napus leaves after 6 dpi. In addition, the gene expression of six plant defense-related genes, namely, PR-1, PR-2 (a marker for SA signaling), PR-3, PDF 1.2 (a marker for JA/ET signaling), CHI620, and CHI570, for both genotypes were analyzed in the leaves of B. napus and R. alboglabra after treatment with TH12 or CF and compared with the non-treated ones. The qRT-PCR results showed that the PR-1 and PR-2 expression levels increased in E. cruciferarum-infected leaves, but decreased in the TH12-treated leaves compared with leaves treated with CF. The expression levels of PR-3 and PDF1.2 decreased in plants infected by E. cruciferarum. However, expression levels increased when the leaves were treated with TH12. For the first time, we disclosed the nature of gene expression in B. napus and R. alboglabra to explore the resistance pathways in the leaves of both genotypes infected and non-infected by powdery mildew and inoculated or non-inoculated with elicitor factors. Results suggested that R. alboglabra exhibited resistance to powdery mildew disease, and the application of T. harzianum and its CF are a useful tool to facilitate new protection methods for resist or susceptible plants.

No MeSH data available.


Related in: MedlinePlus

Expression of defense-related genes of B. napus AACC and R. alboglabra RRCC genotypes of 6-weeks-old inoculated with suspensions 10 ml of TH12 and its cell-free culture filtrate (CF) separately treatedby soil drenching 1 day before infection by pressing diseased leaves by powdery mildew onto leaves. Three potted for each time non-inoculated served as health plants.Leaves were collected 1, 2, 4, 6, 8 and 10 days post infection. Total RNA was extracted, and cDNA was synthesized. Expression levels of the PR-1, PR-2, PDF1.2 (glucanase; BGL2), PR-3 (basic chitinase), CHI620 and CHI570 (chitinase) genes were monitored by qRT-PCR. The expression levels of genes were compared with the expression level of GAPDH.
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pone.0142177.g006: Expression of defense-related genes of B. napus AACC and R. alboglabra RRCC genotypes of 6-weeks-old inoculated with suspensions 10 ml of TH12 and its cell-free culture filtrate (CF) separately treatedby soil drenching 1 day before infection by pressing diseased leaves by powdery mildew onto leaves. Three potted for each time non-inoculated served as health plants.Leaves were collected 1, 2, 4, 6, 8 and 10 days post infection. Total RNA was extracted, and cDNA was synthesized. Expression levels of the PR-1, PR-2, PDF1.2 (glucanase; BGL2), PR-3 (basic chitinase), CHI620 and CHI570 (chitinase) genes were monitored by qRT-PCR. The expression levels of genes were compared with the expression level of GAPDH.

Mentions: We determined the expression levels of the gene-related pathogens in B. napus and R. alboglabra infected by E. cruciferarum 1 day after treatment with TH12 and CF. The gene expression levels were higher in the treated plants compared with non-treated plants at 1, 2, 4, 6, 8, and 10 dpi. Plants treated with CF suspension showed greater effect, wherein the PR-1 and PR-2 gene expression levels were up-regulated by 419.83-fold (from 1.7-fold to 836.12-fold) and 45.52-fold (from 1.9-fold to 86.5-fold) at 1 dpi, respectively, in R. alboglabra infected compared with the non-treated plants, The CF treatment had a weaker effect on B. napus infection (Fig 6A and 6B). The PR1 gene expression levels in infected B. napus treated with CF were up-regulated by 14.28-fold (from 1.4-fold to 46.20-fold) at 1 dpi compared with non-treated (Fig 6A). The PR-2 gene expression levels in infected B. napus treated with CF were up-regulated by 27.68-fold (from 2.3-fold to 63.67-fold) at 10 dpi compared with non-treated (Fig 6B). The TH12 treatment had the weakest effect, affecting PR-1 and PR-2 genes in R. alboglabra infected were up-regulated by 129.41-fold at 1 dpi and 10.96-fold at 2 dpi, respectively (Fig 6A and 6B). TH12 treatment affected on expression levels PR-1 and PR-2 in B. napus were up-regulated by 20.41-fold and 8.52-fold at 2 dpi, respectively, compared with the non-treated ones (Fig 6A and 6B).


Systemic Resistance to Powdery Mildew in Brassica napus (AACC) and Raphanus alboglabra (RRCC) by Trichoderma harzianum TH12.

Alkooranee JT, Yin Y, Aledan TR, Jiang Y, Lu G, Wu J, Li M - PLoS ONE (2015)

Expression of defense-related genes of B. napus AACC and R. alboglabra RRCC genotypes of 6-weeks-old inoculated with suspensions 10 ml of TH12 and its cell-free culture filtrate (CF) separately treatedby soil drenching 1 day before infection by pressing diseased leaves by powdery mildew onto leaves. Three potted for each time non-inoculated served as health plants.Leaves were collected 1, 2, 4, 6, 8 and 10 days post infection. Total RNA was extracted, and cDNA was synthesized. Expression levels of the PR-1, PR-2, PDF1.2 (glucanase; BGL2), PR-3 (basic chitinase), CHI620 and CHI570 (chitinase) genes were monitored by qRT-PCR. The expression levels of genes were compared with the expression level of GAPDH.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4634854&req=5

pone.0142177.g006: Expression of defense-related genes of B. napus AACC and R. alboglabra RRCC genotypes of 6-weeks-old inoculated with suspensions 10 ml of TH12 and its cell-free culture filtrate (CF) separately treatedby soil drenching 1 day before infection by pressing diseased leaves by powdery mildew onto leaves. Three potted for each time non-inoculated served as health plants.Leaves were collected 1, 2, 4, 6, 8 and 10 days post infection. Total RNA was extracted, and cDNA was synthesized. Expression levels of the PR-1, PR-2, PDF1.2 (glucanase; BGL2), PR-3 (basic chitinase), CHI620 and CHI570 (chitinase) genes were monitored by qRT-PCR. The expression levels of genes were compared with the expression level of GAPDH.
Mentions: We determined the expression levels of the gene-related pathogens in B. napus and R. alboglabra infected by E. cruciferarum 1 day after treatment with TH12 and CF. The gene expression levels were higher in the treated plants compared with non-treated plants at 1, 2, 4, 6, 8, and 10 dpi. Plants treated with CF suspension showed greater effect, wherein the PR-1 and PR-2 gene expression levels were up-regulated by 419.83-fold (from 1.7-fold to 836.12-fold) and 45.52-fold (from 1.9-fold to 86.5-fold) at 1 dpi, respectively, in R. alboglabra infected compared with the non-treated plants, The CF treatment had a weaker effect on B. napus infection (Fig 6A and 6B). The PR1 gene expression levels in infected B. napus treated with CF were up-regulated by 14.28-fold (from 1.4-fold to 46.20-fold) at 1 dpi compared with non-treated (Fig 6A). The PR-2 gene expression levels in infected B. napus treated with CF were up-regulated by 27.68-fold (from 2.3-fold to 63.67-fold) at 10 dpi compared with non-treated (Fig 6B). The TH12 treatment had the weakest effect, affecting PR-1 and PR-2 genes in R. alboglabra infected were up-regulated by 129.41-fold at 1 dpi and 10.96-fold at 2 dpi, respectively (Fig 6A and 6B). TH12 treatment affected on expression levels PR-1 and PR-2 in B. napus were up-regulated by 20.41-fold and 8.52-fold at 2 dpi, respectively, compared with the non-treated ones (Fig 6A and 6B).

Bottom Line: In addition, the gene expression of six plant defense-related genes, namely, PR-1, PR-2 (a marker for SA signaling), PR-3, PDF 1.2 (a marker for JA/ET signaling), CHI620, and CHI570, for both genotypes were analyzed in the leaves of B. napus and R. alboglabra after treatment with TH12 or CF and compared with the non-treated ones.The qRT-PCR results showed that the PR-1 and PR-2 expression levels increased in E. cruciferarum-infected leaves, but decreased in the TH12-treated leaves compared with leaves treated with CF.The expression levels of PR-3 and PDF1.2 decreased in plants infected by E. cruciferarum.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT
Trichoderma harzianum TH12 is a microbial pesticide for certain rapeseed diseases. The mechanism of systemic resistance induced by TH12 or its cell-free culture filtrate (CF) in Brassica napus (AACC) and Raphanus alboglabra (RRCC) to powdery mildew disease caused by ascomycete Erysiphe cruciferarum was investigated. In this study, we conducted the first large-scale global study on the cellular and molecular aspects of B. napus and R. alboglabra infected with E. cruciferarum. The histological study showed the resistance of R. alboglabra to powdery mildew disease. The growth of fungal colonies was not observed on R. alboglabra leaves at 1, 2, 4, 6, 8, and 10 days post-inoculation (dpi), whereas this was clearly observed on B. napus leaves after 6 dpi. In addition, the gene expression of six plant defense-related genes, namely, PR-1, PR-2 (a marker for SA signaling), PR-3, PDF 1.2 (a marker for JA/ET signaling), CHI620, and CHI570, for both genotypes were analyzed in the leaves of B. napus and R. alboglabra after treatment with TH12 or CF and compared with the non-treated ones. The qRT-PCR results showed that the PR-1 and PR-2 expression levels increased in E. cruciferarum-infected leaves, but decreased in the TH12-treated leaves compared with leaves treated with CF. The expression levels of PR-3 and PDF1.2 decreased in plants infected by E. cruciferarum. However, expression levels increased when the leaves were treated with TH12. For the first time, we disclosed the nature of gene expression in B. napus and R. alboglabra to explore the resistance pathways in the leaves of both genotypes infected and non-infected by powdery mildew and inoculated or non-inoculated with elicitor factors. Results suggested that R. alboglabra exhibited resistance to powdery mildew disease, and the application of T. harzianum and its CF are a useful tool to facilitate new protection methods for resist or susceptible plants.

No MeSH data available.


Related in: MedlinePlus