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Apolipoprotein A1 -75 G/A and +83 C/T polymorphisms and renal cancer risk.

Liu Z, Xiao Y, Tang L, Jiang L, Wang Y, Zhang R, Wei Q, Lu Y - Lipids Health Dis (2015)

Bottom Line: Apolipoprotein A1 (ApoA1) is the major apoprotein constituent of high-density lipoprotein that can play important roles in tumor invasion and metastasis.Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing.When stratifying by the distant metastasis status, patients with distant metastasis had a significantly higher frequency of APOA1 -75 AA genotype genotype (OR =2.20, 95% CI = 1.04, 4.68; P = 0.04).

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, West China Hospital, Sichuan University, No 37, Guo Xue Xiang, Chengdu, 610041, China.

ABSTRACT

Background: Apolipoprotein A1 (ApoA1) is the major apoprotein constituent of high-density lipoprotein that can play important roles in tumor invasion and metastasis. The objective of the present study was to evaluate the association of two genetic variants (-75 G/A and +83 C/T) of APOA1 with predisposition to renal cancer.

Methods: A total of 432 subjects, including 216 pathologically-proven renal cancer cases and 216 age- and gender-matched healthy controls, were recruited into this hospital-based case-control study. Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing.

Results: Patients with renal cancer had a significantly higher frequency of APOA1 -75 AA genotype [odds ratio (OR) = 2.10, 95% confidence interval (CI) = 1.18, 3.75; P = 0.01] and APOA1 -75 A allele (OR =1.40, 95% CI = 1.05, 1.87; P = 0.02) than controls. When stratifying by the distant metastasis status, patients with distant metastasis had a significantly higher frequency of APOA1 -75 AA genotype genotype (OR =2.20, 95% CI = 1.04, 4.68; P = 0.04).

Conclusion: This study is, to our knowledge, the first to examine prospectively an increased risk role of APOA1 -75 AA genotype and APOA1 -75 A allele in renal cancer susceptibility.

No MeSH data available.


Related in: MedlinePlus

Electrophoresis in a 3 % agarose gel after the Mspl digested in renal cancer cases and healthy controls
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Fig1: Electrophoresis in a 3 % agarose gel after the Mspl digested in renal cancer cases and healthy controls

Mentions: Genomic DNA was isolated from 20 g/L ethylenediaminetetraacetic acid (EDTA) or sodium citrate anticoagulated 3–5 ml venous blood by the commercially available Qiagen kit (QIAGEN Inc., Valencia, CA, USA) and stored at 4 °C. Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Based on the GenBank reference sequence, the PCR primers designed for the APOA1 -75 G/A and +83 C/T were as follows: 5ʹ-AGG GAC AGA GCT GAT CCT TGA ACT CTT AAG-3ʹ (forward) and 5ʹ-TTA GGG GAC ACC TAG CCC TCA GGA AGA GCA-3ʹ (reverse). The restriction endonuclease enzyme MspI digested the amplified PCR products overnight. Electrophoresis in a 3 % agarose gel followed by ethidium bromide staining and ultraviolet illumination allowed detection of the alleles. The presence of the MspI restriction stie at −75 bp (G allele) and at +83 bp (C allele) in the 433 bp product resulted in four fragments of 45, 66, 113 and 209 bp (Fig. 1). The absence of the restriction site at −75 bp (A allele) resulted in three fragments of 45, 179 and 209 bp (Fig. 1). The absence of the restriction site at +83 bp (T allele) created a larger fragment of 254 bp instead of two fragments of 45 and 209 bp (Fig. 1). For quality control, two independent observers randomly chose 44 samples (22 cases and 22 controls) by computer-generated number scheme. They performed double sampling PCR-RFLP and found no differences, and then confirmed by direct sequencing from Qiagen cleaned up DNA.Fig. 1


Apolipoprotein A1 -75 G/A and +83 C/T polymorphisms and renal cancer risk.

Liu Z, Xiao Y, Tang L, Jiang L, Wang Y, Zhang R, Wei Q, Lu Y - Lipids Health Dis (2015)

Electrophoresis in a 3 % agarose gel after the Mspl digested in renal cancer cases and healthy controls
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4634729&req=5

Fig1: Electrophoresis in a 3 % agarose gel after the Mspl digested in renal cancer cases and healthy controls
Mentions: Genomic DNA was isolated from 20 g/L ethylenediaminetetraacetic acid (EDTA) or sodium citrate anticoagulated 3–5 ml venous blood by the commercially available Qiagen kit (QIAGEN Inc., Valencia, CA, USA) and stored at 4 °C. Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Based on the GenBank reference sequence, the PCR primers designed for the APOA1 -75 G/A and +83 C/T were as follows: 5ʹ-AGG GAC AGA GCT GAT CCT TGA ACT CTT AAG-3ʹ (forward) and 5ʹ-TTA GGG GAC ACC TAG CCC TCA GGA AGA GCA-3ʹ (reverse). The restriction endonuclease enzyme MspI digested the amplified PCR products overnight. Electrophoresis in a 3 % agarose gel followed by ethidium bromide staining and ultraviolet illumination allowed detection of the alleles. The presence of the MspI restriction stie at −75 bp (G allele) and at +83 bp (C allele) in the 433 bp product resulted in four fragments of 45, 66, 113 and 209 bp (Fig. 1). The absence of the restriction site at −75 bp (A allele) resulted in three fragments of 45, 179 and 209 bp (Fig. 1). The absence of the restriction site at +83 bp (T allele) created a larger fragment of 254 bp instead of two fragments of 45 and 209 bp (Fig. 1). For quality control, two independent observers randomly chose 44 samples (22 cases and 22 controls) by computer-generated number scheme. They performed double sampling PCR-RFLP and found no differences, and then confirmed by direct sequencing from Qiagen cleaned up DNA.Fig. 1

Bottom Line: Apolipoprotein A1 (ApoA1) is the major apoprotein constituent of high-density lipoprotein that can play important roles in tumor invasion and metastasis.Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing.When stratifying by the distant metastasis status, patients with distant metastasis had a significantly higher frequency of APOA1 -75 AA genotype genotype (OR =2.20, 95% CI = 1.04, 4.68; P = 0.04).

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, West China Hospital, Sichuan University, No 37, Guo Xue Xiang, Chengdu, 610041, China.

ABSTRACT

Background: Apolipoprotein A1 (ApoA1) is the major apoprotein constituent of high-density lipoprotein that can play important roles in tumor invasion and metastasis. The objective of the present study was to evaluate the association of two genetic variants (-75 G/A and +83 C/T) of APOA1 with predisposition to renal cancer.

Methods: A total of 432 subjects, including 216 pathologically-proven renal cancer cases and 216 age- and gender-matched healthy controls, were recruited into this hospital-based case-control study. Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing.

Results: Patients with renal cancer had a significantly higher frequency of APOA1 -75 AA genotype [odds ratio (OR) = 2.10, 95% confidence interval (CI) = 1.18, 3.75; P = 0.01] and APOA1 -75 A allele (OR =1.40, 95% CI = 1.05, 1.87; P = 0.02) than controls. When stratifying by the distant metastasis status, patients with distant metastasis had a significantly higher frequency of APOA1 -75 AA genotype genotype (OR =2.20, 95% CI = 1.04, 4.68; P = 0.04).

Conclusion: This study is, to our knowledge, the first to examine prospectively an increased risk role of APOA1 -75 AA genotype and APOA1 -75 A allele in renal cancer susceptibility.

No MeSH data available.


Related in: MedlinePlus