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Estrogen treatment enhances neurogenic differentiation of human adipose derived stem cells in vitro.

Razavi S, Razavi MR, Ahmadi N, Kazemi M - Iran J Basic Med Sci (2015)

Bottom Line: Estrogen is a sexual hormone that has prominent effects on reproductive and non-reproductive tissues.Analysis of data show that estradiol treatment can significantly increase proliferation rate of differentiated cells (P<0.05).Immunocytochemical and real time RT-PCR analysis revealed that the expression of precursor and mature neuronal markers (nestin and MAP2) was significantly higher in the E2 treated cell cultures when compared to the untreated cell cultures (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Objectives: Estrogen is a sexual hormone that has prominent effects on reproductive and non-reproductive tissues. The aim of this study is to evaluate the effects of estrogen on the proliferation and neural differentiation of human adipose derived stem cells (ADSCs) during neurogenic differentiation.

Materials and methods: Isolated human ADSCs were trans-differentiated in neural induction medium containing neurobasal medium, N2 and B27 with or without 17β-estradiol (E2) treatment. Proliferation rate and neural differentiation of human ADSCs were assessed using MTT assay, immunostaining and real time RT- PCR analysis, respectively.

Results: Analysis of data show that estradiol treatment can significantly increase proliferation rate of differentiated cells (P<0.05). Immunocytochemical and real time RT-PCR analysis revealed that the expression of precursor and mature neuronal markers (nestin and MAP2) was significantly higher in the E2 treated cell cultures when compared to the untreated cell cultures (P<0.05).

Conclusion: According to our findings, estrogen can promote proliferation and neuronal differentiation of human ADSCs.

No MeSH data available.


Phase contrast image of (A) stem cells derived from human adipose tissue, (B) neurospheres formation, (C) differentiated cells derived from human adipose derived stem cells 14 days after neural induction in untreated cell cultures. It shows bipolar and multipolar cells with elongated processes, Scale bars denote in A= 150 µm, B= 200 µm and in C= 100 µm
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Figure 1: Phase contrast image of (A) stem cells derived from human adipose tissue, (B) neurospheres formation, (C) differentiated cells derived from human adipose derived stem cells 14 days after neural induction in untreated cell cultures. It shows bipolar and multipolar cells with elongated processes, Scale bars denote in A= 150 µm, B= 200 µm and in C= 100 µm

Mentions: After initial plating of human ADSCs, the primary culture appeared to be a mono-layer of flat cells. As the cells approached confluency, they assumed a more spindle-shaped, fibroblastic morphology (Figure 1A). To further characterize these cells, cell surface markers were examined by flow cytometry. Flowcytometry analysis of human ADSCs within 3–5 passages showed that ADSCs CD90, CD105, and CD44 were positive, but CD45, CD34, and CD14 were negative, as previously described (22).


Estrogen treatment enhances neurogenic differentiation of human adipose derived stem cells in vitro.

Razavi S, Razavi MR, Ahmadi N, Kazemi M - Iran J Basic Med Sci (2015)

Phase contrast image of (A) stem cells derived from human adipose tissue, (B) neurospheres formation, (C) differentiated cells derived from human adipose derived stem cells 14 days after neural induction in untreated cell cultures. It shows bipolar and multipolar cells with elongated processes, Scale bars denote in A= 150 µm, B= 200 µm and in C= 100 µm
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4633463&req=5

Figure 1: Phase contrast image of (A) stem cells derived from human adipose tissue, (B) neurospheres formation, (C) differentiated cells derived from human adipose derived stem cells 14 days after neural induction in untreated cell cultures. It shows bipolar and multipolar cells with elongated processes, Scale bars denote in A= 150 µm, B= 200 µm and in C= 100 µm
Mentions: After initial plating of human ADSCs, the primary culture appeared to be a mono-layer of flat cells. As the cells approached confluency, they assumed a more spindle-shaped, fibroblastic morphology (Figure 1A). To further characterize these cells, cell surface markers were examined by flow cytometry. Flowcytometry analysis of human ADSCs within 3–5 passages showed that ADSCs CD90, CD105, and CD44 were positive, but CD45, CD34, and CD14 were negative, as previously described (22).

Bottom Line: Estrogen is a sexual hormone that has prominent effects on reproductive and non-reproductive tissues.Analysis of data show that estradiol treatment can significantly increase proliferation rate of differentiated cells (P<0.05).Immunocytochemical and real time RT-PCR analysis revealed that the expression of precursor and mature neuronal markers (nestin and MAP2) was significantly higher in the E2 treated cell cultures when compared to the untreated cell cultures (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Objectives: Estrogen is a sexual hormone that has prominent effects on reproductive and non-reproductive tissues. The aim of this study is to evaluate the effects of estrogen on the proliferation and neural differentiation of human adipose derived stem cells (ADSCs) during neurogenic differentiation.

Materials and methods: Isolated human ADSCs were trans-differentiated in neural induction medium containing neurobasal medium, N2 and B27 with or without 17β-estradiol (E2) treatment. Proliferation rate and neural differentiation of human ADSCs were assessed using MTT assay, immunostaining and real time RT- PCR analysis, respectively.

Results: Analysis of data show that estradiol treatment can significantly increase proliferation rate of differentiated cells (P<0.05). Immunocytochemical and real time RT-PCR analysis revealed that the expression of precursor and mature neuronal markers (nestin and MAP2) was significantly higher in the E2 treated cell cultures when compared to the untreated cell cultures (P<0.05).

Conclusion: According to our findings, estrogen can promote proliferation and neuronal differentiation of human ADSCs.

No MeSH data available.