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Decellularized kidney in the presence of chondroitin sulfate as a natural 3D scaffold for stem cells.

Rafighdoust A, Shahri NM, Baharara J - Iran J Basic Med Sci (2015)

Bottom Line: In addition, cell accumulation on the scaffold surface as well as cells migration to the depth of kidney formed an epithelium-like structure.Glycosaminoglycan could be a right option for impregnation.It is used for smartification and strengthening of natural scaffolds and induction of some behaviors in stem cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

ABSTRACT

Objectives: Use of biological scaffolds and automating the cells directing process with materials such as growth factors and glycosaminoglycans (GAGs) in a certain path may have beneficial effects in tissue engineering and regenerative medicine in future. In this research, chondroitin sulfate sodium was used for impregnation of the scaffolds. It is a critical component in extracellular matrix and plays an important role in signaling pathway; however, little is known about its role within mammalian development and cell linage specification.

Materials and methods: Due to its porous and appropriate structure and for putting cells in 3D space, the kidney of BALB/c mouse was selected and decellulalized using physical and chemical methods. After decellularization, the scaffold was impregnated in chondroitin sulfate solution (CS) for 24 hr. Then, 60×10(5) human adipose-derived mesenchymal stem cells were seeded on the scaffold to assess their behavior on day 5, 10, 15, 20, and 25.

Results: After 48 hr, DAPI staining approved completed decellularized kidney by 1% SDS (sodium dodecyl sulfate). Migration and establishment of a number of cells to the remaining area of the glomerulus was observed. In addition, cell accumulation on the scaffold surface as well as cells migration to the depth of kidney formed an epithelium-like structure. Up to the day 15, microscopic study of different days of seeding showed the gradual adhesion of large number of cells to the scaffold.

Conclusion: Glycosaminoglycan could be a right option for impregnation. It is used for smartification and strengthening of natural scaffolds and induction of some behaviors in stem cells.

No MeSH data available.


Related in: MedlinePlus

Human Adipose Mesenchymal Stem Cells behavior in no CS scaffolds on day 20 with H& and E staining. Figures A-C illustrate dramatic decrease of cell density with H& and E staining. Flash in Figure A, shows probable destroyed areas by cells. D is nuclei fragmentation and E indicates their condensing. F illustrates controlling scaffold of no cell after 25 days
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Figure 7: Human Adipose Mesenchymal Stem Cells behavior in no CS scaffolds on day 20 with H& and E staining. Figures A-C illustrate dramatic decrease of cell density with H& and E staining. Flash in Figure A, shows probable destroyed areas by cells. D is nuclei fragmentation and E indicates their condensing. F illustrates controlling scaffold of no cell after 25 days

Mentions: Scaffolds analysis has illustrated drastic decrease of cells density. During the day 25, cells were placed in small areas on the scaffold. Some destructions were observed in the scaffold, which possibly was in the place of existence and the path of cells’ penetration. The condensing and fragmentation of nuclei was probably indicator of cells progress to death (Figure 7).


Decellularized kidney in the presence of chondroitin sulfate as a natural 3D scaffold for stem cells.

Rafighdoust A, Shahri NM, Baharara J - Iran J Basic Med Sci (2015)

Human Adipose Mesenchymal Stem Cells behavior in no CS scaffolds on day 20 with H& and E staining. Figures A-C illustrate dramatic decrease of cell density with H& and E staining. Flash in Figure A, shows probable destroyed areas by cells. D is nuclei fragmentation and E indicates their condensing. F illustrates controlling scaffold of no cell after 25 days
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4633462&req=5

Figure 7: Human Adipose Mesenchymal Stem Cells behavior in no CS scaffolds on day 20 with H& and E staining. Figures A-C illustrate dramatic decrease of cell density with H& and E staining. Flash in Figure A, shows probable destroyed areas by cells. D is nuclei fragmentation and E indicates their condensing. F illustrates controlling scaffold of no cell after 25 days
Mentions: Scaffolds analysis has illustrated drastic decrease of cells density. During the day 25, cells were placed in small areas on the scaffold. Some destructions were observed in the scaffold, which possibly was in the place of existence and the path of cells’ penetration. The condensing and fragmentation of nuclei was probably indicator of cells progress to death (Figure 7).

Bottom Line: In addition, cell accumulation on the scaffold surface as well as cells migration to the depth of kidney formed an epithelium-like structure.Glycosaminoglycan could be a right option for impregnation.It is used for smartification and strengthening of natural scaffolds and induction of some behaviors in stem cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

ABSTRACT

Objectives: Use of biological scaffolds and automating the cells directing process with materials such as growth factors and glycosaminoglycans (GAGs) in a certain path may have beneficial effects in tissue engineering and regenerative medicine in future. In this research, chondroitin sulfate sodium was used for impregnation of the scaffolds. It is a critical component in extracellular matrix and plays an important role in signaling pathway; however, little is known about its role within mammalian development and cell linage specification.

Materials and methods: Due to its porous and appropriate structure and for putting cells in 3D space, the kidney of BALB/c mouse was selected and decellulalized using physical and chemical methods. After decellularization, the scaffold was impregnated in chondroitin sulfate solution (CS) for 24 hr. Then, 60×10(5) human adipose-derived mesenchymal stem cells were seeded on the scaffold to assess their behavior on day 5, 10, 15, 20, and 25.

Results: After 48 hr, DAPI staining approved completed decellularized kidney by 1% SDS (sodium dodecyl sulfate). Migration and establishment of a number of cells to the remaining area of the glomerulus was observed. In addition, cell accumulation on the scaffold surface as well as cells migration to the depth of kidney formed an epithelium-like structure. Up to the day 15, microscopic study of different days of seeding showed the gradual adhesion of large number of cells to the scaffold.

Conclusion: Glycosaminoglycan could be a right option for impregnation. It is used for smartification and strengthening of natural scaffolds and induction of some behaviors in stem cells.

No MeSH data available.


Related in: MedlinePlus