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Improved viability of random pattern skin flaps with the use of bone marrow mesenchymal-derived stem cells and chicken embryo extract.

Chehelcheraghi F, Eimani H, Sadraie SH, Torkaman G, Amini A, Shemshadi H, Majd HA - Iran J Basic Med Sci (2015)

Bottom Line: Survival area was 19.54±2 in the CEE group and 17.90±2 in the CEE/BM-MSC group when compared to the rates of the total skin flaps, which were significantly higher than the control group (13.47±2) (P<0.05).The biomechanical assessment showed a slight difference, although there was no statistically significant difference between the experimental groups and the control group (P>0.05).The findings from this study demonstrated that in operative treatment with BM-MSCs and CEE transplantation could promote flap survival, but the biomechanical parameters were not contrasted with a saline injection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Medical Faculty, Baqyiatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objectives: Covering tissue defects using skin flaps is a basic surgical strategy for plastic and reconstructive surgery. The aim of this study was to evaluate the effects of chicken embryo extract (CEE) and bone marrow derived mesenchymal stem cells (BM-MSCs) on random skin flap survival (RSF) in rats. Using chicken embryo extract can be an ideal environment for the growth and proliferation of transplanted cells.

Materials and methods: Forty albino male Wistar rats were divided into 4 groups; each group consisted of 10 rats. BM-MSCs and CEE were transplanted into subcutaneous tissue in the area, where the flap would be examined. On the 7(th) postoperative day, the survival areas of the flaps were measured by using digital imaging with software assistance, and tissue was collected for evaluation.

Results: Survival area was 19.54±2 in the CEE group and 17.90±2 in the CEE/BM-MSC group when compared to the rates of the total skin flaps, which were significantly higher than the control group (13.47±2) (P<0.05). The biomechanical assessment showed a slight difference, although there was no statistically significant difference between the experimental groups and the control group (P>0.05).

Conclusion: The findings from this study demonstrated that in operative treatment with BM-MSCs and CEE transplantation could promote flap survival, but the biomechanical parameters were not contrasted with a saline injection.

No MeSH data available.


Related in: MedlinePlus

Mechanical properties of flaps 1 on day 7. (A) A typical load-deformation curve. The linear region of the load-deformation curve was used to calculate the flap stiffness, and the maximum point of the curve was deemed as the UTS of the flaps. (B) The UTS of the wounds on the flap margins of the control and experimental groups, P-value =0.103. (E) Deformation stiffness and work up of Fmax was of the wounds on the flap margins of the control and experimental groups, P-value> 0.05. For all determinations, the sample size was n=10 and all values were represented as the mean±SEM
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Figure 7: Mechanical properties of flaps 1 on day 7. (A) A typical load-deformation curve. The linear region of the load-deformation curve was used to calculate the flap stiffness, and the maximum point of the curve was deemed as the UTS of the flaps. (B) The UTS of the wounds on the flap margins of the control and experimental groups, P-value =0.103. (E) Deformation stiffness and work up of Fmax was of the wounds on the flap margins of the control and experimental groups, P-value> 0.05. For all determinations, the sample size was n=10 and all values were represented as the mean±SEM

Mentions: The ultimate tensile strength (UTS) in the CEE groups was higher than the other groups, but there was no significant difference between the studied groups (mean±SEM, 14.019±0.51; P-value =0.064). There were no significant differences in the work up of the F max (mean±SEM, 23.04±4.62; P-value=0.106), stiffness (mean±SEM, 0.62±0.061; P-value=0.208), and the deformation (mean±SEM, 8.690±0.80; P-value=0.278) of the flaps between the studied groups (Figure 7).


Improved viability of random pattern skin flaps with the use of bone marrow mesenchymal-derived stem cells and chicken embryo extract.

Chehelcheraghi F, Eimani H, Sadraie SH, Torkaman G, Amini A, Shemshadi H, Majd HA - Iran J Basic Med Sci (2015)

Mechanical properties of flaps 1 on day 7. (A) A typical load-deformation curve. The linear region of the load-deformation curve was used to calculate the flap stiffness, and the maximum point of the curve was deemed as the UTS of the flaps. (B) The UTS of the wounds on the flap margins of the control and experimental groups, P-value =0.103. (E) Deformation stiffness and work up of Fmax was of the wounds on the flap margins of the control and experimental groups, P-value> 0.05. For all determinations, the sample size was n=10 and all values were represented as the mean±SEM
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4633459&req=5

Figure 7: Mechanical properties of flaps 1 on day 7. (A) A typical load-deformation curve. The linear region of the load-deformation curve was used to calculate the flap stiffness, and the maximum point of the curve was deemed as the UTS of the flaps. (B) The UTS of the wounds on the flap margins of the control and experimental groups, P-value =0.103. (E) Deformation stiffness and work up of Fmax was of the wounds on the flap margins of the control and experimental groups, P-value> 0.05. For all determinations, the sample size was n=10 and all values were represented as the mean±SEM
Mentions: The ultimate tensile strength (UTS) in the CEE groups was higher than the other groups, but there was no significant difference between the studied groups (mean±SEM, 14.019±0.51; P-value =0.064). There were no significant differences in the work up of the F max (mean±SEM, 23.04±4.62; P-value=0.106), stiffness (mean±SEM, 0.62±0.061; P-value=0.208), and the deformation (mean±SEM, 8.690±0.80; P-value=0.278) of the flaps between the studied groups (Figure 7).

Bottom Line: Survival area was 19.54±2 in the CEE group and 17.90±2 in the CEE/BM-MSC group when compared to the rates of the total skin flaps, which were significantly higher than the control group (13.47±2) (P<0.05).The biomechanical assessment showed a slight difference, although there was no statistically significant difference between the experimental groups and the control group (P>0.05).The findings from this study demonstrated that in operative treatment with BM-MSCs and CEE transplantation could promote flap survival, but the biomechanical parameters were not contrasted with a saline injection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Medical Faculty, Baqyiatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objectives: Covering tissue defects using skin flaps is a basic surgical strategy for plastic and reconstructive surgery. The aim of this study was to evaluate the effects of chicken embryo extract (CEE) and bone marrow derived mesenchymal stem cells (BM-MSCs) on random skin flap survival (RSF) in rats. Using chicken embryo extract can be an ideal environment for the growth and proliferation of transplanted cells.

Materials and methods: Forty albino male Wistar rats were divided into 4 groups; each group consisted of 10 rats. BM-MSCs and CEE were transplanted into subcutaneous tissue in the area, where the flap would be examined. On the 7(th) postoperative day, the survival areas of the flaps were measured by using digital imaging with software assistance, and tissue was collected for evaluation.

Results: Survival area was 19.54±2 in the CEE group and 17.90±2 in the CEE/BM-MSC group when compared to the rates of the total skin flaps, which were significantly higher than the control group (13.47±2) (P<0.05). The biomechanical assessment showed a slight difference, although there was no statistically significant difference between the experimental groups and the control group (P>0.05).

Conclusion: The findings from this study demonstrated that in operative treatment with BM-MSCs and CEE transplantation could promote flap survival, but the biomechanical parameters were not contrasted with a saline injection.

No MeSH data available.


Related in: MedlinePlus