Limits...
Improved viability of random pattern skin flaps with the use of bone marrow mesenchymal-derived stem cells and chicken embryo extract.

Chehelcheraghi F, Eimani H, Sadraie SH, Torkaman G, Amini A, Shemshadi H, Majd HA - Iran J Basic Med Sci (2015)

Bottom Line: Survival area was 19.54±2 in the CEE group and 17.90±2 in the CEE/BM-MSC group when compared to the rates of the total skin flaps, which were significantly higher than the control group (13.47±2) (P<0.05).The biomechanical assessment showed a slight difference, although there was no statistically significant difference between the experimental groups and the control group (P>0.05).The findings from this study demonstrated that in operative treatment with BM-MSCs and CEE transplantation could promote flap survival, but the biomechanical parameters were not contrasted with a saline injection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Medical Faculty, Baqyiatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objectives: Covering tissue defects using skin flaps is a basic surgical strategy for plastic and reconstructive surgery. The aim of this study was to evaluate the effects of chicken embryo extract (CEE) and bone marrow derived mesenchymal stem cells (BM-MSCs) on random skin flap survival (RSF) in rats. Using chicken embryo extract can be an ideal environment for the growth and proliferation of transplanted cells.

Materials and methods: Forty albino male Wistar rats were divided into 4 groups; each group consisted of 10 rats. BM-MSCs and CEE were transplanted into subcutaneous tissue in the area, where the flap would be examined. On the 7(th) postoperative day, the survival areas of the flaps were measured by using digital imaging with software assistance, and tissue was collected for evaluation.

Results: Survival area was 19.54±2 in the CEE group and 17.90±2 in the CEE/BM-MSC group when compared to the rates of the total skin flaps, which were significantly higher than the control group (13.47±2) (P<0.05). The biomechanical assessment showed a slight difference, although there was no statistically significant difference between the experimental groups and the control group (P>0.05).

Conclusion: The findings from this study demonstrated that in operative treatment with BM-MSCs and CEE transplantation could promote flap survival, but the biomechanical parameters were not contrasted with a saline injection.

No MeSH data available.


Related in: MedlinePlus

Diagram of flap sample site for biomechanical test
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4633459&req=5

Figure 2: Diagram of flap sample site for biomechanical test

Mentions: The biomechanical flap properties of the samples were evaluated as in our previous study (8). Briefly, the rats were euthanized by the inhalation of chloroform at the end of day 7, and skin samples were taken (15 mm of length × 35 mm of breadth). The sampling location was in the center of the distal part of the flap site (incision of the sample passed) (Figure 2). The samples were then placed in a piece of gas packing wound impregnated with saline solution and maintained at -20 °C. Prior to conducting the tensiometry test, the samples were slowly thawed at room temperature. We applied a material testing instrument (Zwickau/Roll Z 2-5-PH 1 F, Germany) for the tensiometry, which was calibrated for each use.


Improved viability of random pattern skin flaps with the use of bone marrow mesenchymal-derived stem cells and chicken embryo extract.

Chehelcheraghi F, Eimani H, Sadraie SH, Torkaman G, Amini A, Shemshadi H, Majd HA - Iran J Basic Med Sci (2015)

Diagram of flap sample site for biomechanical test
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4633459&req=5

Figure 2: Diagram of flap sample site for biomechanical test
Mentions: The biomechanical flap properties of the samples were evaluated as in our previous study (8). Briefly, the rats were euthanized by the inhalation of chloroform at the end of day 7, and skin samples were taken (15 mm of length × 35 mm of breadth). The sampling location was in the center of the distal part of the flap site (incision of the sample passed) (Figure 2). The samples were then placed in a piece of gas packing wound impregnated with saline solution and maintained at -20 °C. Prior to conducting the tensiometry test, the samples were slowly thawed at room temperature. We applied a material testing instrument (Zwickau/Roll Z 2-5-PH 1 F, Germany) for the tensiometry, which was calibrated for each use.

Bottom Line: Survival area was 19.54±2 in the CEE group and 17.90±2 in the CEE/BM-MSC group when compared to the rates of the total skin flaps, which were significantly higher than the control group (13.47±2) (P<0.05).The biomechanical assessment showed a slight difference, although there was no statistically significant difference between the experimental groups and the control group (P>0.05).The findings from this study demonstrated that in operative treatment with BM-MSCs and CEE transplantation could promote flap survival, but the biomechanical parameters were not contrasted with a saline injection.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Medical Faculty, Baqyiatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objectives: Covering tissue defects using skin flaps is a basic surgical strategy for plastic and reconstructive surgery. The aim of this study was to evaluate the effects of chicken embryo extract (CEE) and bone marrow derived mesenchymal stem cells (BM-MSCs) on random skin flap survival (RSF) in rats. Using chicken embryo extract can be an ideal environment for the growth and proliferation of transplanted cells.

Materials and methods: Forty albino male Wistar rats were divided into 4 groups; each group consisted of 10 rats. BM-MSCs and CEE were transplanted into subcutaneous tissue in the area, where the flap would be examined. On the 7(th) postoperative day, the survival areas of the flaps were measured by using digital imaging with software assistance, and tissue was collected for evaluation.

Results: Survival area was 19.54±2 in the CEE group and 17.90±2 in the CEE/BM-MSC group when compared to the rates of the total skin flaps, which were significantly higher than the control group (13.47±2) (P<0.05). The biomechanical assessment showed a slight difference, although there was no statistically significant difference between the experimental groups and the control group (P>0.05).

Conclusion: The findings from this study demonstrated that in operative treatment with BM-MSCs and CEE transplantation could promote flap survival, but the biomechanical parameters were not contrasted with a saline injection.

No MeSH data available.


Related in: MedlinePlus