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Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer.

Li SJ, Wang TS, Qin FN, Huang Z, Liang XH, Gao F, Song Z, Yang ZM - Sci Rep (2015)

Bottom Line: Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst.The developmental rate to newborn in zygote-transfer group is obviously higher than that in blastocyst-transfer group, suggesting that a delay in embryo development and implantation will lead to a decrease of litter size.These results indicated that the window of implantation is differentially regulated in two uterine horns of a recipient by embryos at different stages.

View Article: PubMed Central - PubMed

Affiliation: Colleage of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

ABSTRACT
Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst. This study was to investigate the effect of asynchronous embryo transfer on uterine receptivity in mice. Embryos under different stages were transferred into two oviduct sides of a recipient mouse on day 1 of pseudopregnancy. Our results showed the asynchronously transferred embryos can implant in all groups. Compared to zygote-transfer group, the length of implanted embryos is longer in 8-cell embryo- or blastocyst-transfer group. The levels of Snail and COX-2 immunostaining in blastocyst-transfer group are significantly stronger than that in zygote-transfer group. Embryos in blastocyst-transfer group migrate faster than that in zygote-transfer group within uterus. Blastocysts are in a state of developmental delay after they are transferred into oviducts, and they are reactivated and implanted rapidly in uterus. The developmental rate to newborn in zygote-transfer group is obviously higher than that in blastocyst-transfer group, suggesting that a delay in embryo development and implantation will lead to a decrease of litter size. These results indicated that the window of implantation is differentially regulated in two uterine horns of a recipient by embryos at different stages.

No MeSH data available.


Related in: MedlinePlus

The parturition and litter size of transferred embryos at different stages.(A) The parturition time in zygote-transfer group and blastocyst-transfer group. (B) The litter size in zygote-transfer group and blastocyst-transfer group. n, the number of recipients used. *P < 0.05; error bars, S.E.
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f6: The parturition and litter size of transferred embryos at different stages.(A) The parturition time in zygote-transfer group and blastocyst-transfer group. (B) The litter size in zygote-transfer group and blastocyst-transfer group. n, the number of recipients used. *P < 0.05; error bars, S.E.

Mentions: We wondered whether blastocysts or zygotes transferred into day 1 pseudopregnant oviducts could normally develop to term. We didn’t notice any differences on day 5 between zygote- and blastocyst-transfer groups. Although the time of parturition was similar between two groups (Fig. 6A), the litter size in zygote-transfer group was obviously higher than that of blastocyst-transfer group (Fig. 6B), suggesting that a delay in blastocyst-transfer group led to a decrease of litter size. Our results also indicated that the early stage of embryo was beneficial to pregnancy during embryo transfer.


Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer.

Li SJ, Wang TS, Qin FN, Huang Z, Liang XH, Gao F, Song Z, Yang ZM - Sci Rep (2015)

The parturition and litter size of transferred embryos at different stages.(A) The parturition time in zygote-transfer group and blastocyst-transfer group. (B) The litter size in zygote-transfer group and blastocyst-transfer group. n, the number of recipients used. *P < 0.05; error bars, S.E.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4632121&req=5

f6: The parturition and litter size of transferred embryos at different stages.(A) The parturition time in zygote-transfer group and blastocyst-transfer group. (B) The litter size in zygote-transfer group and blastocyst-transfer group. n, the number of recipients used. *P < 0.05; error bars, S.E.
Mentions: We wondered whether blastocysts or zygotes transferred into day 1 pseudopregnant oviducts could normally develop to term. We didn’t notice any differences on day 5 between zygote- and blastocyst-transfer groups. Although the time of parturition was similar between two groups (Fig. 6A), the litter size in zygote-transfer group was obviously higher than that of blastocyst-transfer group (Fig. 6B), suggesting that a delay in blastocyst-transfer group led to a decrease of litter size. Our results also indicated that the early stage of embryo was beneficial to pregnancy during embryo transfer.

Bottom Line: Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst.The developmental rate to newborn in zygote-transfer group is obviously higher than that in blastocyst-transfer group, suggesting that a delay in embryo development and implantation will lead to a decrease of litter size.These results indicated that the window of implantation is differentially regulated in two uterine horns of a recipient by embryos at different stages.

View Article: PubMed Central - PubMed

Affiliation: Colleage of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

ABSTRACT
Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst. This study was to investigate the effect of asynchronous embryo transfer on uterine receptivity in mice. Embryos under different stages were transferred into two oviduct sides of a recipient mouse on day 1 of pseudopregnancy. Our results showed the asynchronously transferred embryos can implant in all groups. Compared to zygote-transfer group, the length of implanted embryos is longer in 8-cell embryo- or blastocyst-transfer group. The levels of Snail and COX-2 immunostaining in blastocyst-transfer group are significantly stronger than that in zygote-transfer group. Embryos in blastocyst-transfer group migrate faster than that in zygote-transfer group within uterus. Blastocysts are in a state of developmental delay after they are transferred into oviducts, and they are reactivated and implanted rapidly in uterus. The developmental rate to newborn in zygote-transfer group is obviously higher than that in blastocyst-transfer group, suggesting that a delay in embryo development and implantation will lead to a decrease of litter size. These results indicated that the window of implantation is differentially regulated in two uterine horns of a recipient by embryos at different stages.

No MeSH data available.


Related in: MedlinePlus