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Genetic Linkage Mapping of Economically Important Traits in Cultivated Tetraploid Potato (Solanum tuberosum L.).

Massa AN, Manrique-Carpintero NC, Coombs JJ, Zarka DG, Boone AE, Kirk WW, Hackett CA, Bryan GJ, Douches DS - G3 (Bethesda) (2015)

Bottom Line: The resulting genetic maps contained 1972 SNP markers with an average density of 1.36 marker per cM.For vine maturity, the major-effect QTL was located on chromosome 5 with allelic effects from both parents.The identification of markers for P. infestans resistance will enable the introgression of multiple sources of resistance through marker-assisted selection.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, Michigan 48824.

No MeSH data available.


Related in: MedlinePlus

Graph of chromosome 9 showing the genetic location (cM) and the physical position (Mb) of SNP markers. (A)“Jacqueline Lee” (JL), 118 markers. (B) “MSG227-2” (G227-2), 114 markers.
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fig4: Graph of chromosome 9 showing the genetic location (cM) and the physical position (Mb) of SNP markers. (A)“Jacqueline Lee” (JL), 118 markers. (B) “MSG227-2” (G227-2), 114 markers.

Mentions: By comparing the genetic location (cM) with the physical position (Mb) of each marker on chromosome 9, we assessed the concordance between the genetic and physical maps. The resulting graphs displayed the expected shape and fit well with chromosome structure (Figure 4) (Felcher et al. 2012; Sharma et al. 2013). Thus, the detection of a major effect QTL for late blight resistance on the long arm of chromosome 9 of “Jacqueline Lee,” along with the identification of a candidate SNP (solcap_snp_c1_10590) associated to this QTL, provides strong evidence for the location of genes controlling the trait. Whether a single gene or multiple genes from this region are responsible for late blight resistance requires further investigation. The phenotypic resistance in the MSL603 population departed from a 1:1 segregation, with a bias toward susceptibility. A single gene with strong environmental effects, multiple R-genes with both qualitative and quantitative effects, conditional QTL (Li et al. 2012), or other genetic factors underlying the trait could explain this segregation pattern. In addition to the major QTL on chromosome 9, the probability of a minor effect QTL on the distal end of chromosome 11, close to a cluster of NB-LRR genes (Jupe et al. 2012) cannot be ruled out. The occurrence of this minor effect QTL however needs to be confirmed, as only one year (2002) was statistically supported (data not shown).


Genetic Linkage Mapping of Economically Important Traits in Cultivated Tetraploid Potato (Solanum tuberosum L.).

Massa AN, Manrique-Carpintero NC, Coombs JJ, Zarka DG, Boone AE, Kirk WW, Hackett CA, Bryan GJ, Douches DS - G3 (Bethesda) (2015)

Graph of chromosome 9 showing the genetic location (cM) and the physical position (Mb) of SNP markers. (A)“Jacqueline Lee” (JL), 118 markers. (B) “MSG227-2” (G227-2), 114 markers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4632055&req=5

fig4: Graph of chromosome 9 showing the genetic location (cM) and the physical position (Mb) of SNP markers. (A)“Jacqueline Lee” (JL), 118 markers. (B) “MSG227-2” (G227-2), 114 markers.
Mentions: By comparing the genetic location (cM) with the physical position (Mb) of each marker on chromosome 9, we assessed the concordance between the genetic and physical maps. The resulting graphs displayed the expected shape and fit well with chromosome structure (Figure 4) (Felcher et al. 2012; Sharma et al. 2013). Thus, the detection of a major effect QTL for late blight resistance on the long arm of chromosome 9 of “Jacqueline Lee,” along with the identification of a candidate SNP (solcap_snp_c1_10590) associated to this QTL, provides strong evidence for the location of genes controlling the trait. Whether a single gene or multiple genes from this region are responsible for late blight resistance requires further investigation. The phenotypic resistance in the MSL603 population departed from a 1:1 segregation, with a bias toward susceptibility. A single gene with strong environmental effects, multiple R-genes with both qualitative and quantitative effects, conditional QTL (Li et al. 2012), or other genetic factors underlying the trait could explain this segregation pattern. In addition to the major QTL on chromosome 9, the probability of a minor effect QTL on the distal end of chromosome 11, close to a cluster of NB-LRR genes (Jupe et al. 2012) cannot be ruled out. The occurrence of this minor effect QTL however needs to be confirmed, as only one year (2002) was statistically supported (data not shown).

Bottom Line: The resulting genetic maps contained 1972 SNP markers with an average density of 1.36 marker per cM.For vine maturity, the major-effect QTL was located on chromosome 5 with allelic effects from both parents.The identification of markers for P. infestans resistance will enable the introgression of multiple sources of resistance through marker-assisted selection.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, Michigan 48824.

No MeSH data available.


Related in: MedlinePlus