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Transcriptome sequencing of purple petal spot region in tree peony reveals differentially expressed anthocyanin structural genes

View Article: PubMed Central

ABSTRACT

The pigmented cells in defined region of a petal constitute the petal spots. Petal spots attract pollinators and are found in many angiosperm families. Several cultivars of tree peony contain a single red or purple spot at the base of petal that makes the flower more attractive for the ornamental market. So far, the understanding of the molecular mechanism of spot formation is inadequate. In this study, we sequenced the transcriptome of the purple spot and the white non-spot of tree peony flower. We assembled and annotated 67,892 unigenes. Comparative analyses of the two transcriptomes showed 1,573 differentially expressed genes, among which 933 were up-regulated, and 640 were down-regulated in the purple spot. Subsequently, we examined four anthocyanin structural genes, including PsCHS, PsF3′H, PsDFR, and PsANS, which expressed at a significantly higher level in the purple spot than in the white non-spot. We further validated the digital expression data using quantitative real-time PCR. Our result uncovered transcriptome variance between the spot and non-spot of tree peony flower, and revealed that the co-expression of four anthocyanin structural genes was responsible for spot pigment in tree peony. The data will further help to unravel the genetic mechanism of peony flower spot formation.

No MeSH data available.


Length distribution of assembled unigenes. Please use a single paragraph for each.
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Figure 2: Length distribution of assembled unigenes. Please use a single paragraph for each.

Mentions: Sequencing projects generated 6.95 G and 7.46 G raw data from spot and non-spot libraries, respectively. De novo assembly using Trinity Software generated 133,153 transcripts with an N50 of 1,451, and 67,892 unigenes with an N50 of 1,138. A total of 42,860 unigenes (63.13%) were between 200 and 500 nt in length; 11,873 unigenes (17.49%) were between 500 and 1000 nt; and 13,159 unigenes (19.38%) were longer than 1000 nt (Figure 2).


Transcriptome sequencing of purple petal spot region in tree peony reveals differentially expressed anthocyanin structural genes
Length distribution of assembled unigenes. Please use a single paragraph for each.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631938&req=5

Figure 2: Length distribution of assembled unigenes. Please use a single paragraph for each.
Mentions: Sequencing projects generated 6.95 G and 7.46 G raw data from spot and non-spot libraries, respectively. De novo assembly using Trinity Software generated 133,153 transcripts with an N50 of 1,451, and 67,892 unigenes with an N50 of 1,138. A total of 42,860 unigenes (63.13%) were between 200 and 500 nt in length; 11,873 unigenes (17.49%) were between 500 and 1000 nt; and 13,159 unigenes (19.38%) were longer than 1000 nt (Figure 2).

View Article: PubMed Central

ABSTRACT

The pigmented cells in defined region of a petal constitute the petal spots. Petal spots attract pollinators and are found in many angiosperm families. Several cultivars of tree peony contain a single red or purple spot at the base of petal that makes the flower more attractive for the ornamental market. So far, the understanding of the molecular mechanism of spot formation is inadequate. In this study, we sequenced the transcriptome of the purple spot and the white non-spot of tree peony flower. We assembled and annotated 67,892 unigenes. Comparative analyses of the two transcriptomes showed 1,573 differentially expressed genes, among which 933 were up-regulated, and 640 were down-regulated in the purple spot. Subsequently, we examined four anthocyanin structural genes, including PsCHS, PsF3′H, PsDFR, and PsANS, which expressed at a significantly higher level in the purple spot than in the white non-spot. We further validated the digital expression data using quantitative real-time PCR. Our result uncovered transcriptome variance between the spot and non-spot of tree peony flower, and revealed that the co-expression of four anthocyanin structural genes was responsible for spot pigment in tree peony. The data will further help to unravel the genetic mechanism of peony flower spot formation.

No MeSH data available.