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Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells.

Kumar N, Richter J, Cutts J, Bush KT, Trujillo C, Nigam SK, Gaasterland T, Brafman D, Willert K - Elife (2015)

Bottom Line: One way to mitigate this risk is to develop expandable progenitor cell populations with restricted differentiation potential.Here, we used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties.Interestingly, IMP cells fail to differentiate into other mesodermally-derived tissues, including blood and heart, suggesting that these cells are restricted to an intermediate mesodermal fate.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, United States.

ABSTRACT
The field of tissue engineering entered a new era with the development of human pluripotent stem cells (hPSCs), which are capable of unlimited expansion whilst retaining the potential to differentiate into all mature cell populations. However, these cells harbor significant risks, including tumor formation upon transplantation. One way to mitigate this risk is to develop expandable progenitor cell populations with restricted differentiation potential. Here, we used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties. This cell population, referred to as intermediate mesodermal progenitor (IMP) cells, is capable of unlimited expansion, lacks tumor formation potential, and, upon appropriate stimulation, readily acquires properties of a sub-population of kidney cells. Interestingly, IMP cells fail to differentiate into other mesodermally-derived tissues, including blood and heart, suggesting that these cells are restricted to an intermediate mesodermal fate.

No MeSH data available.


Related in: MedlinePlus

QPCR analysis of MP cells derived from Hues 9 and BJ RiPS.Low expression of SOX1 demonstrates that MP cells are not committed to the EC lineages. All statistical comparisons are made to the pluripotent (ES or hPS) sample. *p < 0.05, **p < 0.005.DOI:http://dx.doi.org/10.7554/eLife.08413.016
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fig3s10: QPCR analysis of MP cells derived from Hues 9 and BJ RiPS.Low expression of SOX1 demonstrates that MP cells are not committed to the EC lineages. All statistical comparisons are made to the pluripotent (ES or hPS) sample. *p < 0.05, **p < 0.005.DOI:http://dx.doi.org/10.7554/eLife.08413.016


Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells.

Kumar N, Richter J, Cutts J, Bush KT, Trujillo C, Nigam SK, Gaasterland T, Brafman D, Willert K - Elife (2015)

QPCR analysis of MP cells derived from Hues 9 and BJ RiPS.Low expression of SOX1 demonstrates that MP cells are not committed to the EC lineages. All statistical comparisons are made to the pluripotent (ES or hPS) sample. *p < 0.05, **p < 0.005.DOI:http://dx.doi.org/10.7554/eLife.08413.016
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631902&req=5

fig3s10: QPCR analysis of MP cells derived from Hues 9 and BJ RiPS.Low expression of SOX1 demonstrates that MP cells are not committed to the EC lineages. All statistical comparisons are made to the pluripotent (ES or hPS) sample. *p < 0.05, **p < 0.005.DOI:http://dx.doi.org/10.7554/eLife.08413.016
Bottom Line: One way to mitigate this risk is to develop expandable progenitor cell populations with restricted differentiation potential.Here, we used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties.Interestingly, IMP cells fail to differentiate into other mesodermally-derived tissues, including blood and heart, suggesting that these cells are restricted to an intermediate mesodermal fate.

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, United States.

ABSTRACT
The field of tissue engineering entered a new era with the development of human pluripotent stem cells (hPSCs), which are capable of unlimited expansion whilst retaining the potential to differentiate into all mature cell populations. However, these cells harbor significant risks, including tumor formation upon transplantation. One way to mitigate this risk is to develop expandable progenitor cell populations with restricted differentiation potential. Here, we used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties. This cell population, referred to as intermediate mesodermal progenitor (IMP) cells, is capable of unlimited expansion, lacks tumor formation potential, and, upon appropriate stimulation, readily acquires properties of a sub-population of kidney cells. Interestingly, IMP cells fail to differentiate into other mesodermally-derived tissues, including blood and heart, suggesting that these cells are restricted to an intermediate mesodermal fate.

No MeSH data available.


Related in: MedlinePlus