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Antiamnesic Effect of Actinidia arguta Extract Intake in a Mouse Model of TMT-Induced Learning and Memory Dysfunction.

Ha JS, Jin DE, Park SK, Park CH, Seung TW, Bae DW, Kim DO, Heo HJ - Evid Based Complement Alternat Med (2015)

Bottom Line: EFAA not only showed the inhibition of AChE activity and the decline of malondialdehyde (MDA) level as a sign of lipid peroxidation but also presented the increase of the superoxide dismutase (SOD) level and the decrease of the oxidized glutathione (GSSG)/total glutathione (GSH + GSSG) ratio.Finally, the phenolics in EFAA were identified using liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry, and four main phenolics, such as quinic acid, chlorogenic acid, caffeoyl hexose, and quercetin-3-glucoside, were identified.These results suggest that EFAA containing physiological phenolics might enhance drug-induced amnesia through AChE inhibition and neuroprotection.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
The antiamnesic effects of ethyl acetate fraction from Actinidia arguta (EFAA) on trimethyltin- (TMT-) induced memory impairment were investigated to find the possibility of functional food substances. EFAA showed a potent AChE inhibitory effect (IC50 = 53 μg/mL) and efficient neuroprotection against H2O2-induced oxidative stress. The administration of EFAA significantly decreased TMT-induced cognitive deficit in Y-maze, passive avoidance, and Morris water maze (MWM) tests. After the behavioral tests, the antioxidant activities were confirmed using mice brain tissues. EFAA not only showed the inhibition of AChE activity and the decline of malondialdehyde (MDA) level as a sign of lipid peroxidation but also presented the increase of the superoxide dismutase (SOD) level and the decrease of the oxidized glutathione (GSSG)/total glutathione (GSH + GSSG) ratio. Finally, the phenolics in EFAA were identified using liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry, and four main phenolics, such as quinic acid, chlorogenic acid, caffeoyl hexose, and quercetin-3-glucoside, were identified. These results suggest that EFAA containing physiological phenolics might enhance drug-induced amnesia through AChE inhibition and neuroprotection.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effect of ethyl acetate fraction from Actinidia arguta (EFAA) on cellular AChE. Inhibition was expressed as a percentage of enzyme activity inhibited with the control value (100%). Results shown are mean ± SD (n = 3). Data were statistically considered at p < 0.05, and different letters in graph represent statistical difference.
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fig1: Inhibitory effect of ethyl acetate fraction from Actinidia arguta (EFAA) on cellular AChE. Inhibition was expressed as a percentage of enzyme activity inhibited with the control value (100%). Results shown are mean ± SD (n = 3). Data were statistically considered at p < 0.05, and different letters in graph represent statistical difference.

Mentions: EFAA showed a significant AChE inhibitory effect similar to 1 μM of tacrine (positive control) (Figure 1). Tacrine showed the highest inhibitory effect against AChE (63.79%), and most of the EFAA groups significantly inhibited the AChE activity and showed an IC50 value of 53 μg/mL. Neurodegenerative diseases are related to reduced ACh levels as well as relatively high AChE levels resulting from the loss of cholinergic neurons [1]. The phenolics of a natural plant were reported to have an AChE inhibitory effect [3], and kiwifruit belonging to Actinidia spp. showed a high AChE inhibitory effect in in vitro analysis [20]. Therefore, the EFAA might be helpful in improving cognitive dysfunction through inhibition of AChE.


Antiamnesic Effect of Actinidia arguta Extract Intake in a Mouse Model of TMT-Induced Learning and Memory Dysfunction.

Ha JS, Jin DE, Park SK, Park CH, Seung TW, Bae DW, Kim DO, Heo HJ - Evid Based Complement Alternat Med (2015)

Inhibitory effect of ethyl acetate fraction from Actinidia arguta (EFAA) on cellular AChE. Inhibition was expressed as a percentage of enzyme activity inhibited with the control value (100%). Results shown are mean ± SD (n = 3). Data were statistically considered at p < 0.05, and different letters in graph represent statistical difference.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4631892&req=5

fig1: Inhibitory effect of ethyl acetate fraction from Actinidia arguta (EFAA) on cellular AChE. Inhibition was expressed as a percentage of enzyme activity inhibited with the control value (100%). Results shown are mean ± SD (n = 3). Data were statistically considered at p < 0.05, and different letters in graph represent statistical difference.
Mentions: EFAA showed a significant AChE inhibitory effect similar to 1 μM of tacrine (positive control) (Figure 1). Tacrine showed the highest inhibitory effect against AChE (63.79%), and most of the EFAA groups significantly inhibited the AChE activity and showed an IC50 value of 53 μg/mL. Neurodegenerative diseases are related to reduced ACh levels as well as relatively high AChE levels resulting from the loss of cholinergic neurons [1]. The phenolics of a natural plant were reported to have an AChE inhibitory effect [3], and kiwifruit belonging to Actinidia spp. showed a high AChE inhibitory effect in in vitro analysis [20]. Therefore, the EFAA might be helpful in improving cognitive dysfunction through inhibition of AChE.

Bottom Line: EFAA not only showed the inhibition of AChE activity and the decline of malondialdehyde (MDA) level as a sign of lipid peroxidation but also presented the increase of the superoxide dismutase (SOD) level and the decrease of the oxidized glutathione (GSSG)/total glutathione (GSH + GSSG) ratio.Finally, the phenolics in EFAA were identified using liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry, and four main phenolics, such as quinic acid, chlorogenic acid, caffeoyl hexose, and quercetin-3-glucoside, were identified.These results suggest that EFAA containing physiological phenolics might enhance drug-induced amnesia through AChE inhibition and neuroprotection.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
The antiamnesic effects of ethyl acetate fraction from Actinidia arguta (EFAA) on trimethyltin- (TMT-) induced memory impairment were investigated to find the possibility of functional food substances. EFAA showed a potent AChE inhibitory effect (IC50 = 53 μg/mL) and efficient neuroprotection against H2O2-induced oxidative stress. The administration of EFAA significantly decreased TMT-induced cognitive deficit in Y-maze, passive avoidance, and Morris water maze (MWM) tests. After the behavioral tests, the antioxidant activities were confirmed using mice brain tissues. EFAA not only showed the inhibition of AChE activity and the decline of malondialdehyde (MDA) level as a sign of lipid peroxidation but also presented the increase of the superoxide dismutase (SOD) level and the decrease of the oxidized glutathione (GSSG)/total glutathione (GSH + GSSG) ratio. Finally, the phenolics in EFAA were identified using liquid chromatography coupled with hybrid triple quadrupole-linear ion trap mass spectrometry, and four main phenolics, such as quinic acid, chlorogenic acid, caffeoyl hexose, and quercetin-3-glucoside, were identified. These results suggest that EFAA containing physiological phenolics might enhance drug-induced amnesia through AChE inhibition and neuroprotection.

No MeSH data available.


Related in: MedlinePlus