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In Vivo Evaluation of the Antiasthmatic, Antitussive, and Expectorant Activities and Chemical Components of Three Elaeagnus Leaves.

Ge Y, Zhang F, Qin Q, Shang Y, Wan D - Evid Based Complement Alternat Med (2015)

Bottom Line: The leaf of Elaeagnus lanceolata and Elaeagnus henryi as well as Elaeagnus pungens has been documented as an effective herb for the treatment of asthma and chronic bronchitis in traditional clinical medicine.There were no significant differences in the pharmacological actions between the three Elaeagnus leaves.These effects were the important evidence for the traditional use of E. henryi leaf and E. lanceolata leaf as well as E. pungens to treat asthma and chronic bronchitis.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, South-Central University for Nationalities, Wuhan 430074, China.

ABSTRACT
The leaf of Elaeagnus lanceolata and Elaeagnus henryi as well as Elaeagnus pungens has been documented as an effective herb for the treatment of asthma and chronic bronchitis in traditional clinical medicine. This study was aimed at evaluating the antiasthmatic, antitussive, and expectorant activities of the water extracts from the three plants in vivo and analyzing their chemical components by HPLC-DAD. At the medium and high doses, the water extracts of three Elaeagnus leaves significantly prolonged the preconvulsive time (P < 0.01) in guinea pigs, lengthened the latent period of cough (P < 0.01) and decreased the cough frequency caused by aqueous ammonia in mice (P < 0.01), and enhanced tracheal phenol red output in mice (P < 0.01). There were no significant differences in the pharmacological actions between the three Elaeagnus leaves. Moreover, there was more similarity on overlap peaks in the range of retention time from 10 to 40 min by HPLC and many peaks that belonged to flavonoids compounds. It suggested that the main constituents of the three Elaeagnus leaves were flavonoid for the pharmacological activities. These effects were the important evidence for the traditional use of E. henryi leaf and E. lanceolata leaf as well as E. pungens to treat asthma and chronic bronchitis.

No MeSH data available.


Related in: MedlinePlus

HPLC-DAD chromatographies of E. pungens leaf and monomeric compounds C1 (kaempferol 3-O-α-rhamnopyranosyl-(1→2)[α-rhamnopyran-osyl-(1→6)]-β-D-galactopyranoside, tR = 20.600 min), C3 (kaempferol 3-O-(6′′-O-E-p-coumaryl)-β-D-glucopyranoside, tR = 38.763 min).
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fig4: HPLC-DAD chromatographies of E. pungens leaf and monomeric compounds C1 (kaempferol 3-O-α-rhamnopyranosyl-(1→2)[α-rhamnopyran-osyl-(1→6)]-β-D-galactopyranoside, tR = 20.600 min), C3 (kaempferol 3-O-(6′′-O-E-p-coumaryl)-β-D-glucopyranoside, tR = 38.763 min).

Mentions: The chromatographies of E. pungens, E. lanceolata, and E. henryi leaves by HPLC were shown in Figures 4–6. We found that the peaks at the retention time (tR) of 20.650 min and 38.733 min in the E. pungens leaf (Figure 4) were consistent with the monomeric compounds of C1 (kaempferol 3-O-α-rhamnopyranosyl-(1→2)[α-rhamnopyranosyl-(1→6)]-β-D-galactopyranoside, tR = 20.600 min) and C3 (kaempferol 3-O-(6′′-O-E-p-coumaryl)-β-D-glucopyranoside, tR = 38.763 min), respectively. Compared to the monomeric compounds, the overlap ratio of the absorptive curves by DAD was up to 0.97~0.99. In Figure 5, the overlap peak was tR 38.797 min (C3) in the E. lanceolata leaf and three overlaps peaks was tR 20.683 min (C1), 23.157 min (C2, kaempferol 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside, tR = 23.100 min), and 38.87 min (C3) in the E. henryi leaf (Figure 6). Moreover, there was more similarity on overlap peaks between 10 and 40 min (Figure 7) and the peaks belonging to flavonoids compounds and characteristic absorptive wavelengths were also listed in Table 1.


In Vivo Evaluation of the Antiasthmatic, Antitussive, and Expectorant Activities and Chemical Components of Three Elaeagnus Leaves.

Ge Y, Zhang F, Qin Q, Shang Y, Wan D - Evid Based Complement Alternat Med (2015)

HPLC-DAD chromatographies of E. pungens leaf and monomeric compounds C1 (kaempferol 3-O-α-rhamnopyranosyl-(1→2)[α-rhamnopyran-osyl-(1→6)]-β-D-galactopyranoside, tR = 20.600 min), C3 (kaempferol 3-O-(6′′-O-E-p-coumaryl)-β-D-glucopyranoside, tR = 38.763 min).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4631888&req=5

fig4: HPLC-DAD chromatographies of E. pungens leaf and monomeric compounds C1 (kaempferol 3-O-α-rhamnopyranosyl-(1→2)[α-rhamnopyran-osyl-(1→6)]-β-D-galactopyranoside, tR = 20.600 min), C3 (kaempferol 3-O-(6′′-O-E-p-coumaryl)-β-D-glucopyranoside, tR = 38.763 min).
Mentions: The chromatographies of E. pungens, E. lanceolata, and E. henryi leaves by HPLC were shown in Figures 4–6. We found that the peaks at the retention time (tR) of 20.650 min and 38.733 min in the E. pungens leaf (Figure 4) were consistent with the monomeric compounds of C1 (kaempferol 3-O-α-rhamnopyranosyl-(1→2)[α-rhamnopyranosyl-(1→6)]-β-D-galactopyranoside, tR = 20.600 min) and C3 (kaempferol 3-O-(6′′-O-E-p-coumaryl)-β-D-glucopyranoside, tR = 38.763 min), respectively. Compared to the monomeric compounds, the overlap ratio of the absorptive curves by DAD was up to 0.97~0.99. In Figure 5, the overlap peak was tR 38.797 min (C3) in the E. lanceolata leaf and three overlaps peaks was tR 20.683 min (C1), 23.157 min (C2, kaempferol 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside, tR = 23.100 min), and 38.87 min (C3) in the E. henryi leaf (Figure 6). Moreover, there was more similarity on overlap peaks between 10 and 40 min (Figure 7) and the peaks belonging to flavonoids compounds and characteristic absorptive wavelengths were also listed in Table 1.

Bottom Line: The leaf of Elaeagnus lanceolata and Elaeagnus henryi as well as Elaeagnus pungens has been documented as an effective herb for the treatment of asthma and chronic bronchitis in traditional clinical medicine.There were no significant differences in the pharmacological actions between the three Elaeagnus leaves.These effects were the important evidence for the traditional use of E. henryi leaf and E. lanceolata leaf as well as E. pungens to treat asthma and chronic bronchitis.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, South-Central University for Nationalities, Wuhan 430074, China.

ABSTRACT
The leaf of Elaeagnus lanceolata and Elaeagnus henryi as well as Elaeagnus pungens has been documented as an effective herb for the treatment of asthma and chronic bronchitis in traditional clinical medicine. This study was aimed at evaluating the antiasthmatic, antitussive, and expectorant activities of the water extracts from the three plants in vivo and analyzing their chemical components by HPLC-DAD. At the medium and high doses, the water extracts of three Elaeagnus leaves significantly prolonged the preconvulsive time (P < 0.01) in guinea pigs, lengthened the latent period of cough (P < 0.01) and decreased the cough frequency caused by aqueous ammonia in mice (P < 0.01), and enhanced tracheal phenol red output in mice (P < 0.01). There were no significant differences in the pharmacological actions between the three Elaeagnus leaves. Moreover, there was more similarity on overlap peaks in the range of retention time from 10 to 40 min by HPLC and many peaks that belonged to flavonoids compounds. It suggested that the main constituents of the three Elaeagnus leaves were flavonoid for the pharmacological activities. These effects were the important evidence for the traditional use of E. henryi leaf and E. lanceolata leaf as well as E. pungens to treat asthma and chronic bronchitis.

No MeSH data available.


Related in: MedlinePlus