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Hyperactivity and attention deficits in mice with decreased levels of stress-inducible phosphoprotein 1 (STIP1).

Beraldo FH, Thomas A, Kolisnyk B, Hirata PH, De Jaeger X, Martyn AC, Fan J, Goncalves DF, Cowan MF, Masood T, Martins VR, Gros R, Prado VF, Prado MA - Dis Model Mech (2015)

Bottom Line: We found that increased STIP1 regulates the abundance of Hsp70 and Hsp90, whereas reduced STIP1 does not affect Hsp70, Hsp90 or the prion protein.We conclude that reduced STIP1 levels can contribute to phenotypes related to ASD.However, future experiments are needed to define whether it is decreased chaperone capacity or impaired prion protein signaling that contributes to these phenotypes.

View Article: PubMed Central - PubMed

Affiliation: Robarts Research Institute, The University of Western Ontario, London, Ontario N6A5B7, Canada fberaldo@robarts.ca vprado@robarts.ca mprado@robarts.ca.

No MeSH data available.


Related in: MedlinePlus

Spatial memory in STI1TGA and STI1−/+ mice. For the tests, n=14 STI1+/+ and 14 STI1TGA mice were used to test spatial memory in STI1TGA mice and n=11 STI1+/+ and 11 STI1−/+ for STI1−/+ mice. (A) Latency to find the platform. (B) Distance traveled. (C) Speed for STI1TGA mice. (D) Percentage time spent by STI1TGA mice and controls in target quadrant (T) and in opposite (O), right (R) and left (L) quadrants was measured on day 5 in a 60 s probe trial with the platform removed. (E) Latency to find the platform. (F) Distance traveled. (G) Speed for STI1−/+ mice. (H) Percentage time spent by STI1−/+ mice and controls in each quadrant was measured on day 5 in a 60 s probe trial with the platform removed. Results are presented as means±s.e.m.; data were analyzed and compared by two-way ANOVA; ***P<0.001 and ****P<0.0001 compared with time spent in target quadrant.
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DMM022525F5: Spatial memory in STI1TGA and STI1−/+ mice. For the tests, n=14 STI1+/+ and 14 STI1TGA mice were used to test spatial memory in STI1TGA mice and n=11 STI1+/+ and 11 STI1−/+ for STI1−/+ mice. (A) Latency to find the platform. (B) Distance traveled. (C) Speed for STI1TGA mice. (D) Percentage time spent by STI1TGA mice and controls in target quadrant (T) and in opposite (O), right (R) and left (L) quadrants was measured on day 5 in a 60 s probe trial with the platform removed. (E) Latency to find the platform. (F) Distance traveled. (G) Speed for STI1−/+ mice. (H) Percentage time spent by STI1−/+ mice and controls in each quadrant was measured on day 5 in a 60 s probe trial with the platform removed. Results are presented as means±s.e.m.; data were analyzed and compared by two-way ANOVA; ***P<0.001 and ****P<0.0001 compared with time spent in target quadrant.

Mentions: Next, we investigated spatial navigation memory in both Stip1 mutant mice using the Morris water maze (MWM). Neither STI1TGA nor STI1−/+ mice presented deficits in acquisition or retrieval of spatial memory in the MWM. For both STI1TGA and STI1−/+, performance during the 4-day acquisition phase was indistinguishable from their wild-type controls in terms of latency to find the target (Fig. 5A; RM-ANOVA F(1,13)=0.062, P=0.806) or speed (Fig. 5C; F(1,10)=0.215, P=0.652). When spatial memory retrieval was performed on the day-5 probe trial, again no differences were observed between STI1TGA and STI1−/+ mice, compared with their wild-type controls, for time spent investigating the target quadrant (Fig. 5D; F(1,13)=1.046, P=0.3251) or latency (Fig. 5E; F(1,10)=0.215, P=0.294).Fig. 5.


Hyperactivity and attention deficits in mice with decreased levels of stress-inducible phosphoprotein 1 (STIP1).

Beraldo FH, Thomas A, Kolisnyk B, Hirata PH, De Jaeger X, Martyn AC, Fan J, Goncalves DF, Cowan MF, Masood T, Martins VR, Gros R, Prado VF, Prado MA - Dis Model Mech (2015)

Spatial memory in STI1TGA and STI1−/+ mice. For the tests, n=14 STI1+/+ and 14 STI1TGA mice were used to test spatial memory in STI1TGA mice and n=11 STI1+/+ and 11 STI1−/+ for STI1−/+ mice. (A) Latency to find the platform. (B) Distance traveled. (C) Speed for STI1TGA mice. (D) Percentage time spent by STI1TGA mice and controls in target quadrant (T) and in opposite (O), right (R) and left (L) quadrants was measured on day 5 in a 60 s probe trial with the platform removed. (E) Latency to find the platform. (F) Distance traveled. (G) Speed for STI1−/+ mice. (H) Percentage time spent by STI1−/+ mice and controls in each quadrant was measured on day 5 in a 60 s probe trial with the platform removed. Results are presented as means±s.e.m.; data were analyzed and compared by two-way ANOVA; ***P<0.001 and ****P<0.0001 compared with time spent in target quadrant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631792&req=5

DMM022525F5: Spatial memory in STI1TGA and STI1−/+ mice. For the tests, n=14 STI1+/+ and 14 STI1TGA mice were used to test spatial memory in STI1TGA mice and n=11 STI1+/+ and 11 STI1−/+ for STI1−/+ mice. (A) Latency to find the platform. (B) Distance traveled. (C) Speed for STI1TGA mice. (D) Percentage time spent by STI1TGA mice and controls in target quadrant (T) and in opposite (O), right (R) and left (L) quadrants was measured on day 5 in a 60 s probe trial with the platform removed. (E) Latency to find the platform. (F) Distance traveled. (G) Speed for STI1−/+ mice. (H) Percentage time spent by STI1−/+ mice and controls in each quadrant was measured on day 5 in a 60 s probe trial with the platform removed. Results are presented as means±s.e.m.; data were analyzed and compared by two-way ANOVA; ***P<0.001 and ****P<0.0001 compared with time spent in target quadrant.
Mentions: Next, we investigated spatial navigation memory in both Stip1 mutant mice using the Morris water maze (MWM). Neither STI1TGA nor STI1−/+ mice presented deficits in acquisition or retrieval of spatial memory in the MWM. For both STI1TGA and STI1−/+, performance during the 4-day acquisition phase was indistinguishable from their wild-type controls in terms of latency to find the target (Fig. 5A; RM-ANOVA F(1,13)=0.062, P=0.806) or speed (Fig. 5C; F(1,10)=0.215, P=0.652). When spatial memory retrieval was performed on the day-5 probe trial, again no differences were observed between STI1TGA and STI1−/+ mice, compared with their wild-type controls, for time spent investigating the target quadrant (Fig. 5D; F(1,13)=1.046, P=0.3251) or latency (Fig. 5E; F(1,10)=0.215, P=0.294).Fig. 5.

Bottom Line: We found that increased STIP1 regulates the abundance of Hsp70 and Hsp90, whereas reduced STIP1 does not affect Hsp70, Hsp90 or the prion protein.We conclude that reduced STIP1 levels can contribute to phenotypes related to ASD.However, future experiments are needed to define whether it is decreased chaperone capacity or impaired prion protein signaling that contributes to these phenotypes.

View Article: PubMed Central - PubMed

Affiliation: Robarts Research Institute, The University of Western Ontario, London, Ontario N6A5B7, Canada fberaldo@robarts.ca vprado@robarts.ca mprado@robarts.ca.

No MeSH data available.


Related in: MedlinePlus