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Hyperactivity and attention deficits in mice with decreased levels of stress-inducible phosphoprotein 1 (STIP1).

Beraldo FH, Thomas A, Kolisnyk B, Hirata PH, De Jaeger X, Martyn AC, Fan J, Goncalves DF, Cowan MF, Masood T, Martins VR, Gros R, Prado VF, Prado MA - Dis Model Mech (2015)

Bottom Line: We found that increased STIP1 regulates the abundance of Hsp70 and Hsp90, whereas reduced STIP1 does not affect Hsp70, Hsp90 or the prion protein.We conclude that reduced STIP1 levels can contribute to phenotypes related to ASD.However, future experiments are needed to define whether it is decreased chaperone capacity or impaired prion protein signaling that contributes to these phenotypes.

View Article: PubMed Central - PubMed

Affiliation: Robarts Research Institute, The University of Western Ontario, London, Ontario N6A5B7, Canada fberaldo@robarts.ca vprado@robarts.ca mprado@robarts.ca.

No MeSH data available.


Related in: MedlinePlus

Analyses of mRNA for STIP1 partners in STI1+/+, STI1−/+ and STI1TGA mouse brains. (A) STIP1 mRNA expression (n=9 STI1+/+, n=5 STI1TGA and n=4 STI1−/+). (B) PrPC mRNA expression (n=8 STI1+/+, n=4 STI1TGA and n=7 STI1−/+). (C) Hsp70 mRNA expression (n=8 STI1+/+, n=4 STI1TGA and n=7 STI1−/+). (D) Hsp90 mRNA expression (n=3 STI1+/+, n=4 STI1TGA and n=4 STI1−/+). Results are presented as means±s.e.m.; data were analyzed and compared by one-way ANOVA and Bonferroni multiple comparisons post-hoc test; **P<0.001 and ***P<0.0001 compared with control.
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DMM022525F1: Analyses of mRNA for STIP1 partners in STI1+/+, STI1−/+ and STI1TGA mouse brains. (A) STIP1 mRNA expression (n=9 STI1+/+, n=5 STI1TGA and n=4 STI1−/+). (B) PrPC mRNA expression (n=8 STI1+/+, n=4 STI1TGA and n=7 STI1−/+). (C) Hsp70 mRNA expression (n=8 STI1+/+, n=4 STI1TGA and n=7 STI1−/+). (D) Hsp90 mRNA expression (n=3 STI1+/+, n=4 STI1TGA and n=4 STI1−/+). Results are presented as means±s.e.m.; data were analyzed and compared by one-way ANOVA and Bonferroni multiple comparisons post-hoc test; **P<0.001 and ***P<0.0001 compared with control.

Mentions: We initially confirmed previous data to show that STI1−/+ mice present 50% of STIP1 mRNA levels in their brain, whereas STI1TGA mice express almost sixfold more mRNA (Fig. 1A; one-way ANOVA; revealed main effect of genotype F(2.15)=8.521, P<0.0001). In contrast, mRNA levels of known STIP1 interaction partners PrPC (Fig. 1B; one-way ANOVA F(2.16)=1.475, P=0.2580), Hsp70 (Fig. 1C; one-way ANOVA F(2.16)=0.301, P=0.744) and Hsp90 (Fig. 1D; one-way ANOVA F(2.8)=1.249, P=0.337) were not altered in the brain of the two lines, compared with control mice.Fig. 1.


Hyperactivity and attention deficits in mice with decreased levels of stress-inducible phosphoprotein 1 (STIP1).

Beraldo FH, Thomas A, Kolisnyk B, Hirata PH, De Jaeger X, Martyn AC, Fan J, Goncalves DF, Cowan MF, Masood T, Martins VR, Gros R, Prado VF, Prado MA - Dis Model Mech (2015)

Analyses of mRNA for STIP1 partners in STI1+/+, STI1−/+ and STI1TGA mouse brains. (A) STIP1 mRNA expression (n=9 STI1+/+, n=5 STI1TGA and n=4 STI1−/+). (B) PrPC mRNA expression (n=8 STI1+/+, n=4 STI1TGA and n=7 STI1−/+). (C) Hsp70 mRNA expression (n=8 STI1+/+, n=4 STI1TGA and n=7 STI1−/+). (D) Hsp90 mRNA expression (n=3 STI1+/+, n=4 STI1TGA and n=4 STI1−/+). Results are presented as means±s.e.m.; data were analyzed and compared by one-way ANOVA and Bonferroni multiple comparisons post-hoc test; **P<0.001 and ***P<0.0001 compared with control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631792&req=5

DMM022525F1: Analyses of mRNA for STIP1 partners in STI1+/+, STI1−/+ and STI1TGA mouse brains. (A) STIP1 mRNA expression (n=9 STI1+/+, n=5 STI1TGA and n=4 STI1−/+). (B) PrPC mRNA expression (n=8 STI1+/+, n=4 STI1TGA and n=7 STI1−/+). (C) Hsp70 mRNA expression (n=8 STI1+/+, n=4 STI1TGA and n=7 STI1−/+). (D) Hsp90 mRNA expression (n=3 STI1+/+, n=4 STI1TGA and n=4 STI1−/+). Results are presented as means±s.e.m.; data were analyzed and compared by one-way ANOVA and Bonferroni multiple comparisons post-hoc test; **P<0.001 and ***P<0.0001 compared with control.
Mentions: We initially confirmed previous data to show that STI1−/+ mice present 50% of STIP1 mRNA levels in their brain, whereas STI1TGA mice express almost sixfold more mRNA (Fig. 1A; one-way ANOVA; revealed main effect of genotype F(2.15)=8.521, P<0.0001). In contrast, mRNA levels of known STIP1 interaction partners PrPC (Fig. 1B; one-way ANOVA F(2.16)=1.475, P=0.2580), Hsp70 (Fig. 1C; one-way ANOVA F(2.16)=0.301, P=0.744) and Hsp90 (Fig. 1D; one-way ANOVA F(2.8)=1.249, P=0.337) were not altered in the brain of the two lines, compared with control mice.Fig. 1.

Bottom Line: We found that increased STIP1 regulates the abundance of Hsp70 and Hsp90, whereas reduced STIP1 does not affect Hsp70, Hsp90 or the prion protein.We conclude that reduced STIP1 levels can contribute to phenotypes related to ASD.However, future experiments are needed to define whether it is decreased chaperone capacity or impaired prion protein signaling that contributes to these phenotypes.

View Article: PubMed Central - PubMed

Affiliation: Robarts Research Institute, The University of Western Ontario, London, Ontario N6A5B7, Canada fberaldo@robarts.ca vprado@robarts.ca mprado@robarts.ca.

No MeSH data available.


Related in: MedlinePlus