Limits...
A gene expression resource generated by genome-wide lacZ profiling in the mouse.

Tuck E, Estabel J, Oellrich A, Maguire AK, Adissu HA, Souter L, Siragher E, Lillistone C, Green AL, Wardle-Jones H, Carragher DM, Karp NA, Smedley D, Adams NC, Sanger Institute Mouse Genetics ProjectBussell JN, Adams DJ, Ramírez-Solis R, Steel KP, Galli A, White JK - Dis Model Mech (2015)

Bottom Line: Strong agreement was observed, indicating a high degree of specificity in our data.Furthermore, there were 1207 observations of expression of a particular gene in an anatomical structure where Bgee had no data, indicating a large amount of novelty in our data set.Examples of expression data corroborating and extending genotype-phenotype associations and supporting disease gene candidacy are presented to demonstrate the potential of this powerful resource.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK.

No MeSH data available.


Related in: MedlinePlus

Expression data confirm and extend existing knowledge.lacZ reporter gene expression in Sgol2tm1aWtsi/+ mice in the following tissues: (A) testis (T); (B) ovary (black arrows); (C) Peyer's patches (black arrowhead); and (D) mesenteric lymph node (red arrows). (E,F) Expression in Apootm1bWtsi/Y mice in heart (E) and pancreas (F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4631787&req=5

DMM021238F6: Expression data confirm and extend existing knowledge.lacZ reporter gene expression in Sgol2tm1aWtsi/+ mice in the following tissues: (A) testis (T); (B) ovary (black arrows); (C) Peyer's patches (black arrowhead); and (D) mesenteric lymph node (red arrows). (E,F) Expression in Apootm1bWtsi/Y mice in heart (E) and pancreas (F).

Mentions: In addition to the new information provided by those gene expression patterns that have not been reported in adults previously, there is also significant potential to confirm and extend our knowledge of those genes with existing expression data, because the methodology used provides high resolution for a wide range of tissues. One such example is Sgol2, which encodes the cohesion protein shugoshin-like 2. BioGPS (http://biogps.org/#goto=genereport&id=68549) reports strong Sgol2 expression in cell lines and tissues with actively dividing cells, such as the testis and bone marrow, supporting a function for shugoshin-like 2 to protect centromeric cohesion. In addition, low Sgol2 expression levels were associated with meiosis segregation defects in aged eggs (Yun et al., 2014). The function of shugoshin-like 2  is supported by Llano et al. (2008), who reported Sgol2 expression in the testis. We confirmed and extended these observations, reporting lacZ expression in the testis, ovary, Peyer's patches, mesenteric lymph nodes (Fig. 6A-D), thymus and spleen (data not shown) of Sgol2tm1aWtsi/+ mice; all of these tissues contain actively dividing cells.Fig. 6.


A gene expression resource generated by genome-wide lacZ profiling in the mouse.

Tuck E, Estabel J, Oellrich A, Maguire AK, Adissu HA, Souter L, Siragher E, Lillistone C, Green AL, Wardle-Jones H, Carragher DM, Karp NA, Smedley D, Adams NC, Sanger Institute Mouse Genetics ProjectBussell JN, Adams DJ, Ramírez-Solis R, Steel KP, Galli A, White JK - Dis Model Mech (2015)

Expression data confirm and extend existing knowledge.lacZ reporter gene expression in Sgol2tm1aWtsi/+ mice in the following tissues: (A) testis (T); (B) ovary (black arrows); (C) Peyer's patches (black arrowhead); and (D) mesenteric lymph node (red arrows). (E,F) Expression in Apootm1bWtsi/Y mice in heart (E) and pancreas (F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631787&req=5

DMM021238F6: Expression data confirm and extend existing knowledge.lacZ reporter gene expression in Sgol2tm1aWtsi/+ mice in the following tissues: (A) testis (T); (B) ovary (black arrows); (C) Peyer's patches (black arrowhead); and (D) mesenteric lymph node (red arrows). (E,F) Expression in Apootm1bWtsi/Y mice in heart (E) and pancreas (F).
Mentions: In addition to the new information provided by those gene expression patterns that have not been reported in adults previously, there is also significant potential to confirm and extend our knowledge of those genes with existing expression data, because the methodology used provides high resolution for a wide range of tissues. One such example is Sgol2, which encodes the cohesion protein shugoshin-like 2. BioGPS (http://biogps.org/#goto=genereport&id=68549) reports strong Sgol2 expression in cell lines and tissues with actively dividing cells, such as the testis and bone marrow, supporting a function for shugoshin-like 2 to protect centromeric cohesion. In addition, low Sgol2 expression levels were associated with meiosis segregation defects in aged eggs (Yun et al., 2014). The function of shugoshin-like 2  is supported by Llano et al. (2008), who reported Sgol2 expression in the testis. We confirmed and extended these observations, reporting lacZ expression in the testis, ovary, Peyer's patches, mesenteric lymph nodes (Fig. 6A-D), thymus and spleen (data not shown) of Sgol2tm1aWtsi/+ mice; all of these tissues contain actively dividing cells.Fig. 6.

Bottom Line: Strong agreement was observed, indicating a high degree of specificity in our data.Furthermore, there were 1207 observations of expression of a particular gene in an anatomical structure where Bgee had no data, indicating a large amount of novelty in our data set.Examples of expression data corroborating and extending genotype-phenotype associations and supporting disease gene candidacy are presented to demonstrate the potential of this powerful resource.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK.

No MeSH data available.


Related in: MedlinePlus