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A Strontium-Modified Titanium Surface Produced by a New Method and Its Biocompatibility In Vitro.

Liu C, Zhang Y, Wang L, Zhang X, Chen Q, Wu B - PLoS ONE (2015)

Bottom Line: The modified titanium surface had a mesh structure with significantly greater porosity, and approximately5.37±0.35at.% of Sr was incorporated into the surface.These results show that Sr-modification of Ti surfaces may improve bioactivity in vitro.The Sr-modified Ti heat-treated at 700°C exhibited better bioactivity compared with that heated at 600°C.

View Article: PubMed Central - PubMed

Affiliation: Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, P. R. China; College of Stomatology, Southern Medical University, Guangzhou, P. R. China.

ABSTRACT

Objective: To present a new and effective method of producing titanium surfaces modified with strontium and to investigate the surface characteristics and in vitro biocompatibility of titanium (Ti) surfaces modified with strontium (Sr) for bone implant applications.

Materials and methods: Sr-modified Ti surfaces were produced by sequential treatments with NaOH, strontium acetate, heat and water. The surface characteristics and the concentration of the Sr ions released from the samples were examined. Cell adhesion, morphology and growth were investigated using osteoblasts isolated from the calvaria of neonatal Sprague-Dawley rats. Expression of osteogenesis-related genes and proteins was examined to assess the effect of the Sr-modified Ti surfaces on osteoblasts.

Results: The modified titanium surface had a mesh structure with significantly greater porosity, and approximately5.37±0.35at.% of Sr was incorporated into the surface. The hydrophilicity was enhanced by the incorporation of Sr ions and water treatment. The average amounts of Sr released from the Sr-modified plates subjected to water treatment were slight higher than the plates without water treatment. Sr promoted cellular adhesion, spreading and growth compared with untreated Ti surfaces. The Sr-modified Ti plates also promoted expression of osteogenesis-related genes,and expression of OPN and COL-І by osteoblasts. Ti plates heat treated at 700°C showed increased bioactivity in comparison with those treated at 600°C. Water treatment upregulated the expression of osteogenesis-related genes.

Conclusions: These results show that Sr-modification of Ti surfaces may improve bioactivity in vitro. Water treatment has enhanced the response of osteoblasts. The Sr-modified Ti heat-treated at 700°C exhibited better bioactivity compared with that heated at 600°C.

No MeSH data available.


Related in: MedlinePlus

Concentrations of Sr ions measured by ICP-MS, which was released from the Sr-modified plates between day one and day seven.
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pone.0140669.g003: Concentrations of Sr ions measured by ICP-MS, which was released from the Sr-modified plates between day one and day seven.

Mentions: Fig 3 shows the concentration of the Sr ions released from the Sr-modified plates into PBS. It can be seen in Fig 3 that Sr600 and Sr700 released 0.545 ppm and 0.514 ppm of Sr ions within 1 d respectively, then rapidly declined up to 4 days later. In contrast, the Sr600W and Sr700W group released 0.409ppm and 0.407ppm of Sr ions within 1 d respectively, then the released Sr amounts remained relatively constant after 3 days. The average Sr amounts released from Sr700 and Sr700W group are slight higher than Sr600 and Sr600W group with the exception of the burst release at day 1 and day 2. Over all the average amounts released from the Sr-modified plates subjected to water treatment were slight higher than the plates without water treatment.


A Strontium-Modified Titanium Surface Produced by a New Method and Its Biocompatibility In Vitro.

Liu C, Zhang Y, Wang L, Zhang X, Chen Q, Wu B - PLoS ONE (2015)

Concentrations of Sr ions measured by ICP-MS, which was released from the Sr-modified plates between day one and day seven.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631518&req=5

pone.0140669.g003: Concentrations of Sr ions measured by ICP-MS, which was released from the Sr-modified plates between day one and day seven.
Mentions: Fig 3 shows the concentration of the Sr ions released from the Sr-modified plates into PBS. It can be seen in Fig 3 that Sr600 and Sr700 released 0.545 ppm and 0.514 ppm of Sr ions within 1 d respectively, then rapidly declined up to 4 days later. In contrast, the Sr600W and Sr700W group released 0.409ppm and 0.407ppm of Sr ions within 1 d respectively, then the released Sr amounts remained relatively constant after 3 days. The average Sr amounts released from Sr700 and Sr700W group are slight higher than Sr600 and Sr600W group with the exception of the burst release at day 1 and day 2. Over all the average amounts released from the Sr-modified plates subjected to water treatment were slight higher than the plates without water treatment.

Bottom Line: The modified titanium surface had a mesh structure with significantly greater porosity, and approximately5.37±0.35at.% of Sr was incorporated into the surface.These results show that Sr-modification of Ti surfaces may improve bioactivity in vitro.The Sr-modified Ti heat-treated at 700°C exhibited better bioactivity compared with that heated at 600°C.

View Article: PubMed Central - PubMed

Affiliation: Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, P. R. China; College of Stomatology, Southern Medical University, Guangzhou, P. R. China.

ABSTRACT

Objective: To present a new and effective method of producing titanium surfaces modified with strontium and to investigate the surface characteristics and in vitro biocompatibility of titanium (Ti) surfaces modified with strontium (Sr) for bone implant applications.

Materials and methods: Sr-modified Ti surfaces were produced by sequential treatments with NaOH, strontium acetate, heat and water. The surface characteristics and the concentration of the Sr ions released from the samples were examined. Cell adhesion, morphology and growth were investigated using osteoblasts isolated from the calvaria of neonatal Sprague-Dawley rats. Expression of osteogenesis-related genes and proteins was examined to assess the effect of the Sr-modified Ti surfaces on osteoblasts.

Results: The modified titanium surface had a mesh structure with significantly greater porosity, and approximately5.37±0.35at.% of Sr was incorporated into the surface. The hydrophilicity was enhanced by the incorporation of Sr ions and water treatment. The average amounts of Sr released from the Sr-modified plates subjected to water treatment were slight higher than the plates without water treatment. Sr promoted cellular adhesion, spreading and growth compared with untreated Ti surfaces. The Sr-modified Ti plates also promoted expression of osteogenesis-related genes,and expression of OPN and COL-І by osteoblasts. Ti plates heat treated at 700°C showed increased bioactivity in comparison with those treated at 600°C. Water treatment upregulated the expression of osteogenesis-related genes.

Conclusions: These results show that Sr-modification of Ti surfaces may improve bioactivity in vitro. Water treatment has enhanced the response of osteoblasts. The Sr-modified Ti heat-treated at 700°C exhibited better bioactivity compared with that heated at 600°C.

No MeSH data available.


Related in: MedlinePlus