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Differential microRNA Expression in Fast- and Slow-Twitch Skeletal Muscle of Piaractus mesopotamicus during Growth.

Duran BO, Fernandez GJ, Mareco EA, Moraes LN, Salomão RA, Gutierrez de Paula T, Santos VB, Carvalho RF, Dal-Pai-Silvca M - PLoS ONE (2015)

Bottom Line: Our results revealed an inverse correlation between the expression of the miRNAs and their target mRNAs, and there was evidence that miR-1 and miR-206 may regulate the differentiation of myoblasts, whereas miR-133 may regulate the proliferation of these cells. miR-499 was highly expressed in slow-twitch muscle, which suggests its involvement in the specification of the slow phenotype in muscle fibers.The expression of these miRNAs exhibited variations between different development stages and between distinct muscle twitch phenotypes.This work provides the first identification of miRNA expression profiles in pacu skeletal muscle and suggests an important role of these molecules in muscle growth and in the maintenance of the muscle phenotype.

View Article: PubMed Central - PubMed

Affiliation: Department of Morphology, Institute of Biosciences of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil.

ABSTRACT
Pacu (Piaractus mesopotamicus) is a Brazilian fish with a high economic value in pisciculture due to its rusticity and fast growth. Postnatal growth of skeletal muscle in fish occurs by hyperplasia and/or hypertrophy, processes that are dependent on the proliferation and differentiation of myoblasts. A class of small noncoding RNAs, known as microRNAs (miRNAs), represses the expression of target mRNAs, and many studies have demonstrated that miR-1, miR-133, miR-206 and miR-499 regulate different processes in skeletal muscle through the mRNA silencing of hdac4 (histone deacetylase 4), srf (serum response factor), pax7 (paired box 7) and sox6 ((sex determining region Y)-box 6), respectively. The aim of our work was to evaluate the expression of these miRNAs and their putative target mRNAs in fast- and slow-twitch skeletal muscle of pacu during growth. We used pacus in three different development stages: larval (aged 30 days), juvenile (aged 90 days and 150 days) and adult (aged 2 years). To complement our study, we also performed a pacu myoblast cell culture, which allowed us to investigate miRNA expression in the progression from myoblast proliferation to differentiation. Our results revealed an inverse correlation between the expression of the miRNAs and their target mRNAs, and there was evidence that miR-1 and miR-206 may regulate the differentiation of myoblasts, whereas miR-133 may regulate the proliferation of these cells. miR-499 was highly expressed in slow-twitch muscle, which suggests its involvement in the specification of the slow phenotype in muscle fibers. The expression of these miRNAs exhibited variations between different development stages and between distinct muscle twitch phenotypes. This work provides the first identification of miRNA expression profiles in pacu skeletal muscle and suggests an important role of these molecules in muscle growth and in the maintenance of the muscle phenotype.

No MeSH data available.


Related in: MedlinePlus

Relative expression of miR-1 and the hdac4 mRNA in fast- and slow-twitch muscles of pacu during growth.miR-1 and hdac4 mRNA expression was assessed by qPCR in pacu skeletal muscle at different development stages: 30-day-old larvae (30 d), 90-day-old juveniles (90 d), 150-day-old juveniles (150 d) and 2-year-old adults (2 y). The slow-twitch muscle was analyzed only in the 150-day-old and 2-year-old pacus, given the difficulty in isolating this tissue in younger animals (30- and 90-day-old pacus). The data are expressed as the fold change compared with the expression level in the fast-twitch muscle in the 30-day-old pacus or the expression level in the slow-twitch muscle in the 150-day-old pacus. The data are presented as the mean ± SEM (n = 6). The different letters indicate significant differences in expression (p<0.05).
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pone.0141967.g001: Relative expression of miR-1 and the hdac4 mRNA in fast- and slow-twitch muscles of pacu during growth.miR-1 and hdac4 mRNA expression was assessed by qPCR in pacu skeletal muscle at different development stages: 30-day-old larvae (30 d), 90-day-old juveniles (90 d), 150-day-old juveniles (150 d) and 2-year-old adults (2 y). The slow-twitch muscle was analyzed only in the 150-day-old and 2-year-old pacus, given the difficulty in isolating this tissue in younger animals (30- and 90-day-old pacus). The data are expressed as the fold change compared with the expression level in the fast-twitch muscle in the 30-day-old pacus or the expression level in the slow-twitch muscle in the 150-day-old pacus. The data are presented as the mean ± SEM (n = 6). The different letters indicate significant differences in expression (p<0.05).

Mentions: The expression of miR-1 in the fast-twitch muscle was upregulated 10-fold in 2-year-old pacus (p<0.001), unlike the expression of the hdac4 mRNA, which showed a 5-fold decrease in 2-year-old pacus (p<0.001). This variation was also observed in the slow-twitch muscle, wherein miR-1 was upregulated 4-fold in 2-year-old pacus (p<0.001), and the hdac4 mRNA was downregulated 2-fold in these animals (p<0.05) (Fig 1).


Differential microRNA Expression in Fast- and Slow-Twitch Skeletal Muscle of Piaractus mesopotamicus during Growth.

Duran BO, Fernandez GJ, Mareco EA, Moraes LN, Salomão RA, Gutierrez de Paula T, Santos VB, Carvalho RF, Dal-Pai-Silvca M - PLoS ONE (2015)

Relative expression of miR-1 and the hdac4 mRNA in fast- and slow-twitch muscles of pacu during growth.miR-1 and hdac4 mRNA expression was assessed by qPCR in pacu skeletal muscle at different development stages: 30-day-old larvae (30 d), 90-day-old juveniles (90 d), 150-day-old juveniles (150 d) and 2-year-old adults (2 y). The slow-twitch muscle was analyzed only in the 150-day-old and 2-year-old pacus, given the difficulty in isolating this tissue in younger animals (30- and 90-day-old pacus). The data are expressed as the fold change compared with the expression level in the fast-twitch muscle in the 30-day-old pacus or the expression level in the slow-twitch muscle in the 150-day-old pacus. The data are presented as the mean ± SEM (n = 6). The different letters indicate significant differences in expression (p<0.05).
© Copyright Policy
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4631509&req=5

pone.0141967.g001: Relative expression of miR-1 and the hdac4 mRNA in fast- and slow-twitch muscles of pacu during growth.miR-1 and hdac4 mRNA expression was assessed by qPCR in pacu skeletal muscle at different development stages: 30-day-old larvae (30 d), 90-day-old juveniles (90 d), 150-day-old juveniles (150 d) and 2-year-old adults (2 y). The slow-twitch muscle was analyzed only in the 150-day-old and 2-year-old pacus, given the difficulty in isolating this tissue in younger animals (30- and 90-day-old pacus). The data are expressed as the fold change compared with the expression level in the fast-twitch muscle in the 30-day-old pacus or the expression level in the slow-twitch muscle in the 150-day-old pacus. The data are presented as the mean ± SEM (n = 6). The different letters indicate significant differences in expression (p<0.05).
Mentions: The expression of miR-1 in the fast-twitch muscle was upregulated 10-fold in 2-year-old pacus (p<0.001), unlike the expression of the hdac4 mRNA, which showed a 5-fold decrease in 2-year-old pacus (p<0.001). This variation was also observed in the slow-twitch muscle, wherein miR-1 was upregulated 4-fold in 2-year-old pacus (p<0.001), and the hdac4 mRNA was downregulated 2-fold in these animals (p<0.05) (Fig 1).

Bottom Line: Our results revealed an inverse correlation between the expression of the miRNAs and their target mRNAs, and there was evidence that miR-1 and miR-206 may regulate the differentiation of myoblasts, whereas miR-133 may regulate the proliferation of these cells. miR-499 was highly expressed in slow-twitch muscle, which suggests its involvement in the specification of the slow phenotype in muscle fibers.The expression of these miRNAs exhibited variations between different development stages and between distinct muscle twitch phenotypes.This work provides the first identification of miRNA expression profiles in pacu skeletal muscle and suggests an important role of these molecules in muscle growth and in the maintenance of the muscle phenotype.

View Article: PubMed Central - PubMed

Affiliation: Department of Morphology, Institute of Biosciences of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil.

ABSTRACT
Pacu (Piaractus mesopotamicus) is a Brazilian fish with a high economic value in pisciculture due to its rusticity and fast growth. Postnatal growth of skeletal muscle in fish occurs by hyperplasia and/or hypertrophy, processes that are dependent on the proliferation and differentiation of myoblasts. A class of small noncoding RNAs, known as microRNAs (miRNAs), represses the expression of target mRNAs, and many studies have demonstrated that miR-1, miR-133, miR-206 and miR-499 regulate different processes in skeletal muscle through the mRNA silencing of hdac4 (histone deacetylase 4), srf (serum response factor), pax7 (paired box 7) and sox6 ((sex determining region Y)-box 6), respectively. The aim of our work was to evaluate the expression of these miRNAs and their putative target mRNAs in fast- and slow-twitch skeletal muscle of pacu during growth. We used pacus in three different development stages: larval (aged 30 days), juvenile (aged 90 days and 150 days) and adult (aged 2 years). To complement our study, we also performed a pacu myoblast cell culture, which allowed us to investigate miRNA expression in the progression from myoblast proliferation to differentiation. Our results revealed an inverse correlation between the expression of the miRNAs and their target mRNAs, and there was evidence that miR-1 and miR-206 may regulate the differentiation of myoblasts, whereas miR-133 may regulate the proliferation of these cells. miR-499 was highly expressed in slow-twitch muscle, which suggests its involvement in the specification of the slow phenotype in muscle fibers. The expression of these miRNAs exhibited variations between different development stages and between distinct muscle twitch phenotypes. This work provides the first identification of miRNA expression profiles in pacu skeletal muscle and suggests an important role of these molecules in muscle growth and in the maintenance of the muscle phenotype.

No MeSH data available.


Related in: MedlinePlus