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Expression of the Rice Arginase Gene OsARG in Cotton Influences the Morphology and Nitrogen Transition of Seedlings.

Meng Z, Meng Z, Zhang R, Liang C, Wan J, Wang Y, Zhai H, Guo S - PLoS ONE (2015)

Bottom Line: Field experiments indicated that the polyamine and nitrogen content increased by more than two-fold in the T3 generation plants of the transgenic cotton lines ARG-26-2, ARG-26-7, ARG-38-8, and ARG-38-11, as compared with the control plants.After harvesting cotton fibers grown in field conditions, we analyzed the quality of fiber and found that the fiber length was increased in the transgenic lines.Our results indicate that the OsARG gene could potentially be used to improve cotton fiber length traits.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Arginase is the only enzyme capable of producing urea in plants. This enzyme also contributes to many important biological functions during plant growth and development, such as seed development, root development and plant nitrogen using. The unique rice arginase gene OsARG is known to affect nitrogen use efficiency and is also associated with higher yields in rice. In this study, we transformed OsARG into upland cotton R18 by Agrobacterium-mediated genetic transformation and analyzed the function of OsARG in transgenic cotton. Two independent OsARG expression transgenic cotton lines, ARG-26 and ARG-38, were obtained via transformation. Southern blot analysis indicated that two copies and one copy of the OsARG gene were integrated into the ARG-26 and ARG-38 genomes, respectively. Enzyme activity and RNA transcription analysis revealed that the OsARG gene is highly expressed in cotton. The nitric oxide content and the morphology of ARG-26 and ARG-38 seedlings were both affected by expression of the OsARG gene. Field experiments indicated that the polyamine and nitrogen content increased by more than two-fold in the T3 generation plants of the transgenic cotton lines ARG-26-2, ARG-26-7, ARG-38-8, and ARG-38-11, as compared with the control plants. After harvesting cotton fibers grown in field conditions, we analyzed the quality of fiber and found that the fiber length was increased in the transgenic lines. The average cotton fiber length for all of the transgenic cotton lines was two millimeters longer than the fibers of the control plants; the average cotton fiber lengths were 31.94 mm, 32.00 mm, 32.68 mm and 32.84 mm in the ARG-26ARG-26-2, ARG-26-7, ARG-38-8 and ARG-38-11 lines, respectively, but the average fiber length of the control plants was 29.36mm. Our results indicate that the OsARG gene could potentially be used to improve cotton fiber length traits.

No MeSH data available.


Related in: MedlinePlus

Morphology analyses of transgenic cotton seedlings grown on 1/2 MS medium with varying nitrogen content.T4-generation ARG-26 and ARG-38 cotton seedling were cultured on three kinds of growth media:1/2 MS medium, 1/2 MS medium with 4×NH4NO3, and 1/2 MS medium lacking nitrogen. Due to the expression of the OsARG gene in cotton, the seedling height, taproot length, lateral root number, and lateral root length of ARG-26 and ARG-38 plants were changed compared with those of non-transgenic control cotton plants. Both of the transgenic seedling heights were same or increased on 1/2 MS medium and 1/2 MS medium with 4×NH4NO3, but decreased on 1/2 MS medium lacking nitrogen. Both of the transgenic seedling taproot lengths were same or increased on 1/2 MS medium with 4×NH4NO3 and 1/2 MS medium lacking nitrogen, but decreased on 1/2 MS medium. Both of the transgenic seedling lateral root numbers were decreased on 1/2 MS medium,1/2 MS medium with 4×NH4NO3 and 1/2 MS medium lacking nitrogen. Both of the transgenic seedling lateral root lengths were little increased on 1/2 MS medium with 4×NH4NO3, but decreased on 1/2 MS medium and 1/2 MS medium lacking nitrogen. (A) the seedling height of transgenic cotton was analyzed for plants grown on three kinds of growth media. (B) taproot length was analyzed of transgenic cotton was analyzed for plants grown on three kinds of growth media. (C) and (D) the average number and the average length of lateral roots were analyzed for plants grown on three kinds of growth media for plants grown on three kind growth media. ‘WT’ refers to non-transgenic control plants of wild type cotton. *: P<0.05.
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pone.0141530.g006: Morphology analyses of transgenic cotton seedlings grown on 1/2 MS medium with varying nitrogen content.T4-generation ARG-26 and ARG-38 cotton seedling were cultured on three kinds of growth media:1/2 MS medium, 1/2 MS medium with 4×NH4NO3, and 1/2 MS medium lacking nitrogen. Due to the expression of the OsARG gene in cotton, the seedling height, taproot length, lateral root number, and lateral root length of ARG-26 and ARG-38 plants were changed compared with those of non-transgenic control cotton plants. Both of the transgenic seedling heights were same or increased on 1/2 MS medium and 1/2 MS medium with 4×NH4NO3, but decreased on 1/2 MS medium lacking nitrogen. Both of the transgenic seedling taproot lengths were same or increased on 1/2 MS medium with 4×NH4NO3 and 1/2 MS medium lacking nitrogen, but decreased on 1/2 MS medium. Both of the transgenic seedling lateral root numbers were decreased on 1/2 MS medium,1/2 MS medium with 4×NH4NO3 and 1/2 MS medium lacking nitrogen. Both of the transgenic seedling lateral root lengths were little increased on 1/2 MS medium with 4×NH4NO3, but decreased on 1/2 MS medium and 1/2 MS medium lacking nitrogen. (A) the seedling height of transgenic cotton was analyzed for plants grown on three kinds of growth media. (B) taproot length was analyzed of transgenic cotton was analyzed for plants grown on three kinds of growth media. (C) and (D) the average number and the average length of lateral roots were analyzed for plants grown on three kinds of growth media for plants grown on three kind growth media. ‘WT’ refers to non-transgenic control plants of wild type cotton. *: P<0.05.

Mentions: Compared with control plants, the average height of ARG-26 and ARG-38 seedlings were inversely correlated with taproot length (Fig 6A and 6B).When ARG-26 and ARG-38 seedlings were grown on 1/2 MS medium, the average height of the transgenic seedlings were greater than or similar to the height of the control seedlings; the average taproot lengths of the transgenic seedlings were shorter than those measured for control seedlings. However, the average seedling height and the average taproot length of ARG-38 were each greater than those of ARG-26. When ARG-26 and ARG-38 seedlings were grown on 1/2 MS medium with 4×NH4NO3, the average seedling height of ARG-26 was greater than that of the control; the average taproot length of ARG-26 was not significantly different from that of the control. The average height and the average taproot length of the ARG-38 seedlings were inversely correlated with the corresponding measurements for the ARG-26 seedlings. However, when ARG-26 and ARG-38 seedlings were grown on 1/2 MS medium without NH4NO3 and KNO3, the average heights of the ARG-26 and ARG-38 seedlings were shorter than the control seedlings; under these conditions, the average taproot lengths of ARG-26 and ARG-38 were longer than that of the control. No differences in the average height or average taproot length were observed between the ARG-26 and ARG-38 seedlings.


Expression of the Rice Arginase Gene OsARG in Cotton Influences the Morphology and Nitrogen Transition of Seedlings.

Meng Z, Meng Z, Zhang R, Liang C, Wan J, Wang Y, Zhai H, Guo S - PLoS ONE (2015)

Morphology analyses of transgenic cotton seedlings grown on 1/2 MS medium with varying nitrogen content.T4-generation ARG-26 and ARG-38 cotton seedling were cultured on three kinds of growth media:1/2 MS medium, 1/2 MS medium with 4×NH4NO3, and 1/2 MS medium lacking nitrogen. Due to the expression of the OsARG gene in cotton, the seedling height, taproot length, lateral root number, and lateral root length of ARG-26 and ARG-38 plants were changed compared with those of non-transgenic control cotton plants. Both of the transgenic seedling heights were same or increased on 1/2 MS medium and 1/2 MS medium with 4×NH4NO3, but decreased on 1/2 MS medium lacking nitrogen. Both of the transgenic seedling taproot lengths were same or increased on 1/2 MS medium with 4×NH4NO3 and 1/2 MS medium lacking nitrogen, but decreased on 1/2 MS medium. Both of the transgenic seedling lateral root numbers were decreased on 1/2 MS medium,1/2 MS medium with 4×NH4NO3 and 1/2 MS medium lacking nitrogen. Both of the transgenic seedling lateral root lengths were little increased on 1/2 MS medium with 4×NH4NO3, but decreased on 1/2 MS medium and 1/2 MS medium lacking nitrogen. (A) the seedling height of transgenic cotton was analyzed for plants grown on three kinds of growth media. (B) taproot length was analyzed of transgenic cotton was analyzed for plants grown on three kinds of growth media. (C) and (D) the average number and the average length of lateral roots were analyzed for plants grown on three kinds of growth media for plants grown on three kind growth media. ‘WT’ refers to non-transgenic control plants of wild type cotton. *: P<0.05.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4631492&req=5

pone.0141530.g006: Morphology analyses of transgenic cotton seedlings grown on 1/2 MS medium with varying nitrogen content.T4-generation ARG-26 and ARG-38 cotton seedling were cultured on three kinds of growth media:1/2 MS medium, 1/2 MS medium with 4×NH4NO3, and 1/2 MS medium lacking nitrogen. Due to the expression of the OsARG gene in cotton, the seedling height, taproot length, lateral root number, and lateral root length of ARG-26 and ARG-38 plants were changed compared with those of non-transgenic control cotton plants. Both of the transgenic seedling heights were same or increased on 1/2 MS medium and 1/2 MS medium with 4×NH4NO3, but decreased on 1/2 MS medium lacking nitrogen. Both of the transgenic seedling taproot lengths were same or increased on 1/2 MS medium with 4×NH4NO3 and 1/2 MS medium lacking nitrogen, but decreased on 1/2 MS medium. Both of the transgenic seedling lateral root numbers were decreased on 1/2 MS medium,1/2 MS medium with 4×NH4NO3 and 1/2 MS medium lacking nitrogen. Both of the transgenic seedling lateral root lengths were little increased on 1/2 MS medium with 4×NH4NO3, but decreased on 1/2 MS medium and 1/2 MS medium lacking nitrogen. (A) the seedling height of transgenic cotton was analyzed for plants grown on three kinds of growth media. (B) taproot length was analyzed of transgenic cotton was analyzed for plants grown on three kinds of growth media. (C) and (D) the average number and the average length of lateral roots were analyzed for plants grown on three kinds of growth media for plants grown on three kind growth media. ‘WT’ refers to non-transgenic control plants of wild type cotton. *: P<0.05.
Mentions: Compared with control plants, the average height of ARG-26 and ARG-38 seedlings were inversely correlated with taproot length (Fig 6A and 6B).When ARG-26 and ARG-38 seedlings were grown on 1/2 MS medium, the average height of the transgenic seedlings were greater than or similar to the height of the control seedlings; the average taproot lengths of the transgenic seedlings were shorter than those measured for control seedlings. However, the average seedling height and the average taproot length of ARG-38 were each greater than those of ARG-26. When ARG-26 and ARG-38 seedlings were grown on 1/2 MS medium with 4×NH4NO3, the average seedling height of ARG-26 was greater than that of the control; the average taproot length of ARG-26 was not significantly different from that of the control. The average height and the average taproot length of the ARG-38 seedlings were inversely correlated with the corresponding measurements for the ARG-26 seedlings. However, when ARG-26 and ARG-38 seedlings were grown on 1/2 MS medium without NH4NO3 and KNO3, the average heights of the ARG-26 and ARG-38 seedlings were shorter than the control seedlings; under these conditions, the average taproot lengths of ARG-26 and ARG-38 were longer than that of the control. No differences in the average height or average taproot length were observed between the ARG-26 and ARG-38 seedlings.

Bottom Line: Field experiments indicated that the polyamine and nitrogen content increased by more than two-fold in the T3 generation plants of the transgenic cotton lines ARG-26-2, ARG-26-7, ARG-38-8, and ARG-38-11, as compared with the control plants.After harvesting cotton fibers grown in field conditions, we analyzed the quality of fiber and found that the fiber length was increased in the transgenic lines.Our results indicate that the OsARG gene could potentially be used to improve cotton fiber length traits.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
Arginase is the only enzyme capable of producing urea in plants. This enzyme also contributes to many important biological functions during plant growth and development, such as seed development, root development and plant nitrogen using. The unique rice arginase gene OsARG is known to affect nitrogen use efficiency and is also associated with higher yields in rice. In this study, we transformed OsARG into upland cotton R18 by Agrobacterium-mediated genetic transformation and analyzed the function of OsARG in transgenic cotton. Two independent OsARG expression transgenic cotton lines, ARG-26 and ARG-38, were obtained via transformation. Southern blot analysis indicated that two copies and one copy of the OsARG gene were integrated into the ARG-26 and ARG-38 genomes, respectively. Enzyme activity and RNA transcription analysis revealed that the OsARG gene is highly expressed in cotton. The nitric oxide content and the morphology of ARG-26 and ARG-38 seedlings were both affected by expression of the OsARG gene. Field experiments indicated that the polyamine and nitrogen content increased by more than two-fold in the T3 generation plants of the transgenic cotton lines ARG-26-2, ARG-26-7, ARG-38-8, and ARG-38-11, as compared with the control plants. After harvesting cotton fibers grown in field conditions, we analyzed the quality of fiber and found that the fiber length was increased in the transgenic lines. The average cotton fiber length for all of the transgenic cotton lines was two millimeters longer than the fibers of the control plants; the average cotton fiber lengths were 31.94 mm, 32.00 mm, 32.68 mm and 32.84 mm in the ARG-26ARG-26-2, ARG-26-7, ARG-38-8 and ARG-38-11 lines, respectively, but the average fiber length of the control plants was 29.36mm. Our results indicate that the OsARG gene could potentially be used to improve cotton fiber length traits.

No MeSH data available.


Related in: MedlinePlus