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Overexpression of IFITM3 predicts the high risk of lymphatic metastatic recurrence in pN0 esophageal squamous cell carcinoma after Ivor-Lewis esophagectomy.

Jia Y, Zhang M, Jiang W, Zhang Z, Huang S, Wang Z - PeerJ (2015)

Bottom Line: Both IHC and RT-PCR demonstrated that the IFITM3 expression level was significantly higher in tumor tissue than in ANM.Statistical analysis showed a significant correlation of IFITM3 expression with the T status of esophageal cancer (p = 0.015).Conclusions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong University , Jinan, Shandong , China.

ABSTRACT
Background. Recent studies have shown that the aberrant expression of IFITM3 is implicated in the lymph node metastasis of many malignancies. Our research aimed to investigate the expression of IFITM3 in pathological N0 (pN0) esophageal squamous cell carcinoma (ESCC) and its relationship with lymph node metastatic recurrence. Methods. Immunohistochemistry (IHC) was used to examine the expression profile of IFITM3 in 104 pairs of samples. Each pair consisted of ESCC tissue and its adjacent normal mucosa (ANM). This aberrant expression was verified by reverse transcription-polymerase chain reaction (RT-PCR) with 20 tumor specimens with strong immunostaining and their mucosal tissues. In addition, 20 samples of low expression tissues and their ANMs were evaluated. Moreover, the correlations between the IFITM3 expression level and the clinicopathological variables, recurrence risk and overall survival (OS) of patients were analyzed. Results. Both IHC and RT-PCR demonstrated that the IFITM3 expression level was significantly higher in tumor tissue than in ANM. Statistical analysis showed a significant correlation of IFITM3 expression with the T status of esophageal cancer (p = 0.015). In addition, IFITM3 overexpression was demonstrated to be not only an important risk factor of lymphatic metastatic recurrence but a significant prognostic factor in pN0 ESCC (p < 0.005). Conclusions. Even pN0 ESCC patients will still experience lymphatic metastatic recurrence. The IFITM3 gene could be a predictor of lymphatic metastatic recurrence in pN0 ESCC after Ivor-Lewis esophagectomy.

No MeSH data available.


Related in: MedlinePlus

Relative expression level of IFITM3 mRNA was detected by RT-PCR.(M) Molecular marker. (1) and (2) respectively represent the mRNA expression level of β-actin and IFITM3 in tumor tissues with IFITM3 protein overexpression; (3) and (4) represent the mRNA expression level of β-actin and IFITM3 in their ANMs. (5) and (6) respectively represent the mRNA expression level of β-actin and IFITM3 in low IFITM3 protein expressed tumor tissues; (7) and (8) represent this expression level of β-actin and IFITM3 in their ANMs.
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fig-2: Relative expression level of IFITM3 mRNA was detected by RT-PCR.(M) Molecular marker. (1) and (2) respectively represent the mRNA expression level of β-actin and IFITM3 in tumor tissues with IFITM3 protein overexpression; (3) and (4) represent the mRNA expression level of β-actin and IFITM3 in their ANMs. (5) and (6) respectively represent the mRNA expression level of β-actin and IFITM3 in low IFITM3 protein expressed tumor tissues; (7) and (8) represent this expression level of β-actin and IFITM3 in their ANMs.

Mentions: To verify this aberrant upregulation of IFITM3, we examined the mRNA expression level by RT-PCR with 20 pairs of specimens randomly selected from the overexpression group and 20 pairs of tissues that originated from the low expression group. The results showed that the mRNA expression level was consistent with protein expression as demonstrated by IHC (Fig. 2).


Overexpression of IFITM3 predicts the high risk of lymphatic metastatic recurrence in pN0 esophageal squamous cell carcinoma after Ivor-Lewis esophagectomy.

Jia Y, Zhang M, Jiang W, Zhang Z, Huang S, Wang Z - PeerJ (2015)

Relative expression level of IFITM3 mRNA was detected by RT-PCR.(M) Molecular marker. (1) and (2) respectively represent the mRNA expression level of β-actin and IFITM3 in tumor tissues with IFITM3 protein overexpression; (3) and (4) represent the mRNA expression level of β-actin and IFITM3 in their ANMs. (5) and (6) respectively represent the mRNA expression level of β-actin and IFITM3 in low IFITM3 protein expressed tumor tissues; (7) and (8) represent this expression level of β-actin and IFITM3 in their ANMs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631461&req=5

fig-2: Relative expression level of IFITM3 mRNA was detected by RT-PCR.(M) Molecular marker. (1) and (2) respectively represent the mRNA expression level of β-actin and IFITM3 in tumor tissues with IFITM3 protein overexpression; (3) and (4) represent the mRNA expression level of β-actin and IFITM3 in their ANMs. (5) and (6) respectively represent the mRNA expression level of β-actin and IFITM3 in low IFITM3 protein expressed tumor tissues; (7) and (8) represent this expression level of β-actin and IFITM3 in their ANMs.
Mentions: To verify this aberrant upregulation of IFITM3, we examined the mRNA expression level by RT-PCR with 20 pairs of specimens randomly selected from the overexpression group and 20 pairs of tissues that originated from the low expression group. The results showed that the mRNA expression level was consistent with protein expression as demonstrated by IHC (Fig. 2).

Bottom Line: Both IHC and RT-PCR demonstrated that the IFITM3 expression level was significantly higher in tumor tissue than in ANM.Statistical analysis showed a significant correlation of IFITM3 expression with the T status of esophageal cancer (p = 0.015).Conclusions.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong University , Jinan, Shandong , China.

ABSTRACT
Background. Recent studies have shown that the aberrant expression of IFITM3 is implicated in the lymph node metastasis of many malignancies. Our research aimed to investigate the expression of IFITM3 in pathological N0 (pN0) esophageal squamous cell carcinoma (ESCC) and its relationship with lymph node metastatic recurrence. Methods. Immunohistochemistry (IHC) was used to examine the expression profile of IFITM3 in 104 pairs of samples. Each pair consisted of ESCC tissue and its adjacent normal mucosa (ANM). This aberrant expression was verified by reverse transcription-polymerase chain reaction (RT-PCR) with 20 tumor specimens with strong immunostaining and their mucosal tissues. In addition, 20 samples of low expression tissues and their ANMs were evaluated. Moreover, the correlations between the IFITM3 expression level and the clinicopathological variables, recurrence risk and overall survival (OS) of patients were analyzed. Results. Both IHC and RT-PCR demonstrated that the IFITM3 expression level was significantly higher in tumor tissue than in ANM. Statistical analysis showed a significant correlation of IFITM3 expression with the T status of esophageal cancer (p = 0.015). In addition, IFITM3 overexpression was demonstrated to be not only an important risk factor of lymphatic metastatic recurrence but a significant prognostic factor in pN0 ESCC (p < 0.005). Conclusions. Even pN0 ESCC patients will still experience lymphatic metastatic recurrence. The IFITM3 gene could be a predictor of lymphatic metastatic recurrence in pN0 ESCC after Ivor-Lewis esophagectomy.

No MeSH data available.


Related in: MedlinePlus