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Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize.

Chen G, Wang X, Hao J, Yan J, Ding J - PLoS ONE (2015)

Bottom Line: Fifteen candidate genes associated with MRDD resistance were identified, including ten genes with annotated protein encoding functions.The homologous of nine candidate genes were predicted to relate to plant defense in different species based on published results.The results in present study also imply the candidate genes in defense pathway play an important role in resistance to MRDD in maize.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Maize rough dwarf disease (MRDD) is a destructive viral disease in China, which results in 20-30% of the maize yield losses in affected areas and even as high as 100% in severely infected fields. Understanding the genetic basis of resistance will provide important insights for maize breeding program. In this study, a diverse maize population comprising of 527 inbred lines was evaluated in four environments and a genome-wide association study (GWAS) was undertaken with over 556000 SNP markers. Fifteen candidate genes associated with MRDD resistance were identified, including ten genes with annotated protein encoding functions. The homologous of nine candidate genes were predicted to relate to plant defense in different species based on published results. Significant correlation (R2 = 0.79) between the MRDD severity and the number of resistance alleles was observed. Consequently, we have broadened the resistant germplasm to MRDD and identified a number of resistance alleles by GWAS. The results in present study also imply the candidate genes in defense pathway play an important role in resistance to MRDD in maize.

No MeSH data available.


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The joint effect of resistance alleles on resistance to MRDD in association panel.
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pone.0142001.g003: The joint effect of resistance alleles on resistance to MRDD in association panel.

Mentions: To further understand the combined effect of causes of variation in resistance to MRDD, we examined the number of resistance alleles in each line in association panel. For the 17 loci significantly associated with resistance to MRDD, the resistance alleles of the lines ranged from 0 to 15. We regrouped the lines based on the number of resistance alleles they carried. The relationship between MRDD resistance and the number of resistance alleles was estimated with linear regression analysis. On group level, we found the number of resistance alleles was significantly associated with MRDD resistance. As shown in Fig 3, the number of resistance alleles increased and so did mean resistance to MRDD in the group. There was a strong and negative correlation between the MRDD severity and number of resistance alleles (R2 = 0.79). We also analyzed the correlation between MRDD severity of line in association panel and the number of resistance alleles in each line, and similar results were also achieved (S2 Fig).


Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize.

Chen G, Wang X, Hao J, Yan J, Ding J - PLoS ONE (2015)

The joint effect of resistance alleles on resistance to MRDD in association panel.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631334&req=5

pone.0142001.g003: The joint effect of resistance alleles on resistance to MRDD in association panel.
Mentions: To further understand the combined effect of causes of variation in resistance to MRDD, we examined the number of resistance alleles in each line in association panel. For the 17 loci significantly associated with resistance to MRDD, the resistance alleles of the lines ranged from 0 to 15. We regrouped the lines based on the number of resistance alleles they carried. The relationship between MRDD resistance and the number of resistance alleles was estimated with linear regression analysis. On group level, we found the number of resistance alleles was significantly associated with MRDD resistance. As shown in Fig 3, the number of resistance alleles increased and so did mean resistance to MRDD in the group. There was a strong and negative correlation between the MRDD severity and number of resistance alleles (R2 = 0.79). We also analyzed the correlation between MRDD severity of line in association panel and the number of resistance alleles in each line, and similar results were also achieved (S2 Fig).

Bottom Line: Fifteen candidate genes associated with MRDD resistance were identified, including ten genes with annotated protein encoding functions.The homologous of nine candidate genes were predicted to relate to plant defense in different species based on published results.The results in present study also imply the candidate genes in defense pathway play an important role in resistance to MRDD in maize.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.

ABSTRACT
Maize rough dwarf disease (MRDD) is a destructive viral disease in China, which results in 20-30% of the maize yield losses in affected areas and even as high as 100% in severely infected fields. Understanding the genetic basis of resistance will provide important insights for maize breeding program. In this study, a diverse maize population comprising of 527 inbred lines was evaluated in four environments and a genome-wide association study (GWAS) was undertaken with over 556000 SNP markers. Fifteen candidate genes associated with MRDD resistance were identified, including ten genes with annotated protein encoding functions. The homologous of nine candidate genes were predicted to relate to plant defense in different species based on published results. Significant correlation (R2 = 0.79) between the MRDD severity and the number of resistance alleles was observed. Consequently, we have broadened the resistant germplasm to MRDD and identified a number of resistance alleles by GWAS. The results in present study also imply the candidate genes in defense pathway play an important role in resistance to MRDD in maize.

No MeSH data available.


Related in: MedlinePlus