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Automated-immunosensor with centrifugal fluid valves for salivary cortisol measurement.

Yamaguchi M, Katagata H, Tezuka Y, Niwa D, Shetty V - Sens Biosensing Res (2014)

Bottom Line: Incorporated centrifugal fluid valves allow for rapid and repeat washings to remove impurities from the saliva samples.An optical reader and laptop computer automate the immunoassay processes and provide easily accessible digital readouts of salivary cortisol measurements.The receiver operating characteristic (ROC) curve analysis of human saliva samples indicate potential utility for discriminating stress disorders and underscore potential application of the biosensor in stress disorders.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Engineering & Robotics Laboratory, Graduate School of Engineering, Iwate University, 4-3-5 Ueda, Morioka 020-8551, Japan.

ABSTRACT

Point-of-care measurement of the stress hormone cortisol will greatly facilitate the timely diagnosis and management of stress-related disorders. We describe an automated salivary cortisol immunosensor, incorporating centrifugal fluid valves and a disposable disc-chip that allows for truncated reporting of cortisol levels (<15 min). The performance characteristics of the immunosensor are optimized through select blocking agents to prevent the non-specific adsorption of proteins; immunoglobulin G (IgG) polymer for the pad and milk protein for the reservoirs and the flow channels. Incorporated centrifugal fluid valves allow for rapid and repeat washings to remove impurities from the saliva samples. An optical reader and laptop computer automate the immunoassay processes and provide easily accessible digital readouts of salivary cortisol measurements. Linear regression analysis of the calibration curve for the cortisol immunosensor showed 0.92 of coefficient of multiple determination, R (2), and 38.7% of coefficient of variation, CV, for a range of salivary cortisol concentrations between 0.4 and 11.3 ng/mL. The receiver operating characteristic (ROC) curve analysis of human saliva samples indicate potential utility for discriminating stress disorders and underscore potential application of the biosensor in stress disorders. The performance of our salivary cortisol immunosensor approaches laboratory based tests and allows noninvasive, quantitative, and automated analysis of human salivary cortisol levels with reporting times compatible with point-of-care applications.

No MeSH data available.


Related in: MedlinePlus

(A) Block diagram of the salivary cortisol immunosensor, (B) External view of the immunosensor, and (C) Use steps for the immunosensor.
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Figure 3: (A) Block diagram of the salivary cortisol immunosensor, (B) External view of the immunosensor, and (C) Use steps for the immunosensor.

Mentions: The salivary cortisol immunosensor system comprises of the disposable disc-chip with pad, a portable optical reader equipped with an amplifier (H8258, wavelength: 185-680 nm, Hamamatsu Photonics K.K., Japan), a stepping motor with a control unit (PK525HPMA, 0.36° step angle, Oriental Motor Co., Ltd., Japan), and a laptop computer (FMVLCE70B, Fujitsu Ltd., Japan, Fig. 3A and B) that controls the component processes. The assembled unit is cube-shaped with an edge length of 190 mm. The disposable disc-chips are easily exchanged by removing a simple fixing screw.


Automated-immunosensor with centrifugal fluid valves for salivary cortisol measurement.

Yamaguchi M, Katagata H, Tezuka Y, Niwa D, Shetty V - Sens Biosensing Res (2014)

(A) Block diagram of the salivary cortisol immunosensor, (B) External view of the immunosensor, and (C) Use steps for the immunosensor.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631318&req=5

Figure 3: (A) Block diagram of the salivary cortisol immunosensor, (B) External view of the immunosensor, and (C) Use steps for the immunosensor.
Mentions: The salivary cortisol immunosensor system comprises of the disposable disc-chip with pad, a portable optical reader equipped with an amplifier (H8258, wavelength: 185-680 nm, Hamamatsu Photonics K.K., Japan), a stepping motor with a control unit (PK525HPMA, 0.36° step angle, Oriental Motor Co., Ltd., Japan), and a laptop computer (FMVLCE70B, Fujitsu Ltd., Japan, Fig. 3A and B) that controls the component processes. The assembled unit is cube-shaped with an edge length of 190 mm. The disposable disc-chips are easily exchanged by removing a simple fixing screw.

Bottom Line: Incorporated centrifugal fluid valves allow for rapid and repeat washings to remove impurities from the saliva samples.An optical reader and laptop computer automate the immunoassay processes and provide easily accessible digital readouts of salivary cortisol measurements.The receiver operating characteristic (ROC) curve analysis of human saliva samples indicate potential utility for discriminating stress disorders and underscore potential application of the biosensor in stress disorders.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Engineering & Robotics Laboratory, Graduate School of Engineering, Iwate University, 4-3-5 Ueda, Morioka 020-8551, Japan.

ABSTRACT

Point-of-care measurement of the stress hormone cortisol will greatly facilitate the timely diagnosis and management of stress-related disorders. We describe an automated salivary cortisol immunosensor, incorporating centrifugal fluid valves and a disposable disc-chip that allows for truncated reporting of cortisol levels (<15 min). The performance characteristics of the immunosensor are optimized through select blocking agents to prevent the non-specific adsorption of proteins; immunoglobulin G (IgG) polymer for the pad and milk protein for the reservoirs and the flow channels. Incorporated centrifugal fluid valves allow for rapid and repeat washings to remove impurities from the saliva samples. An optical reader and laptop computer automate the immunoassay processes and provide easily accessible digital readouts of salivary cortisol measurements. Linear regression analysis of the calibration curve for the cortisol immunosensor showed 0.92 of coefficient of multiple determination, R (2), and 38.7% of coefficient of variation, CV, for a range of salivary cortisol concentrations between 0.4 and 11.3 ng/mL. The receiver operating characteristic (ROC) curve analysis of human saliva samples indicate potential utility for discriminating stress disorders and underscore potential application of the biosensor in stress disorders. The performance of our salivary cortisol immunosensor approaches laboratory based tests and allows noninvasive, quantitative, and automated analysis of human salivary cortisol levels with reporting times compatible with point-of-care applications.

No MeSH data available.


Related in: MedlinePlus