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The effect of Lactobacillus casei extract on cervical cancer cell lines.

Kim SN, Lee WM, Park KS, Kim JB, Han DJ, Bae J - Contemp Oncol (Pozn) (2015)

Bottom Line: L. casei extract had no significant effect on the growth rate of the two cell lines.Anti-cancer drugs alone induced growth inhibition, but there was no synergistic effect of L. casei extract on growth inhibition.L. casei extract does not have a potent effect on the viability of cervical cancer cells in vitro.

View Article: PubMed Central - PubMed

Affiliation: Konkuk University Medical Center, Seoul, South Korea.

ABSTRACT

Aim of the study: Lactobacillus casei (L. casei) has been shown to inhibit the proliferation of several types of cancer in vivo, but its effect on cervical cells has not been reported. We incubated cells of the human cervical cell lines Caski and HeLa with extracts of L. casei and investigated its effects on the growth of the cells and possible synergy with anticancer drugs.

Material and methods: Cell-free extracts of L. casei were prepared and purified. Cultures of Caski and HeLa cells adhering to tissue culture plates were treated with L. casei extract. The effects of L. casei extract on the growth of cancer cells and its possible synergy with anti-cancer drugs in cervical cancer cell lines were investigated. The cells were treated with L. casei extract alone, anti-cancer drugs alone [doxorubicin, paclitaxel, 5-fluorouracil (5-FU), and cisplatin], or L. casei extract plus anti-cancer drugs.

Results: L. casei extract had no significant effect on the growth rate of the two cell lines. Anti-cancer drugs alone induced growth inhibition, but there was no synergistic effect of L. casei extract on growth inhibition.

Conclusions: L. casei extract does not have a potent effect on the viability of cervical cancer cells in vitro. In addition, L. casei extract has no synergistic effect on the inhibition of growth of cancer cells in the presence of anti-cancer drugs.

No MeSH data available.


Related in: MedlinePlus

Growth rate of CaSki and HeLa cells after treatment with Lactobacillus casei extract and anti-cancer drugs. A) Caski cells. B) HeLa cells. Aliquots of 5 × 105 cells were seeded in Dulbecco's modified Eagle's medium (DMEM) containing 10% foetal bovine serum (FBS). Cells were treated with distilled water, followed by L. casei extract (10 μg/ml), anti-cancer drugs (doxorubicin 10 nM, 5-fluorouracil (5-FU) 10 nM, paclitaxel 10 nM, cisplatin 10 nM) or L. casei extract (10 μg/ml) plus anti-cancer drugs (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM) for 72 hours. Cells were then stained with trypan blue to determine viability. Viable cell numbers in the treated cultures are shown relative to controls
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Figure 0003: Growth rate of CaSki and HeLa cells after treatment with Lactobacillus casei extract and anti-cancer drugs. A) Caski cells. B) HeLa cells. Aliquots of 5 × 105 cells were seeded in Dulbecco's modified Eagle's medium (DMEM) containing 10% foetal bovine serum (FBS). Cells were treated with distilled water, followed by L. casei extract (10 μg/ml), anti-cancer drugs (doxorubicin 10 nM, 5-fluorouracil (5-FU) 10 nM, paclitaxel 10 nM, cisplatin 10 nM) or L. casei extract (10 μg/ml) plus anti-cancer drugs (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM) for 72 hours. Cells were then stained with trypan blue to determine viability. Viable cell numbers in the treated cultures are shown relative to controls

Mentions: To measure any enhanced effect of L. casei extract plus anti-cancer drugs, CaSki and HeLa cells were treated with L. casei extract alone, anti-cancer drugs alone (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM), or L. casei extract plus anti-cancer drugs (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM). All of the anti-cancer drugs inhibited the growth of these cells, but L. casei extract had no additional effect (Fig. 3A, B).


The effect of Lactobacillus casei extract on cervical cancer cell lines.

Kim SN, Lee WM, Park KS, Kim JB, Han DJ, Bae J - Contemp Oncol (Pozn) (2015)

Growth rate of CaSki and HeLa cells after treatment with Lactobacillus casei extract and anti-cancer drugs. A) Caski cells. B) HeLa cells. Aliquots of 5 × 105 cells were seeded in Dulbecco's modified Eagle's medium (DMEM) containing 10% foetal bovine serum (FBS). Cells were treated with distilled water, followed by L. casei extract (10 μg/ml), anti-cancer drugs (doxorubicin 10 nM, 5-fluorouracil (5-FU) 10 nM, paclitaxel 10 nM, cisplatin 10 nM) or L. casei extract (10 μg/ml) plus anti-cancer drugs (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM) for 72 hours. Cells were then stained with trypan blue to determine viability. Viable cell numbers in the treated cultures are shown relative to controls
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631296&req=5

Figure 0003: Growth rate of CaSki and HeLa cells after treatment with Lactobacillus casei extract and anti-cancer drugs. A) Caski cells. B) HeLa cells. Aliquots of 5 × 105 cells were seeded in Dulbecco's modified Eagle's medium (DMEM) containing 10% foetal bovine serum (FBS). Cells were treated with distilled water, followed by L. casei extract (10 μg/ml), anti-cancer drugs (doxorubicin 10 nM, 5-fluorouracil (5-FU) 10 nM, paclitaxel 10 nM, cisplatin 10 nM) or L. casei extract (10 μg/ml) plus anti-cancer drugs (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM) for 72 hours. Cells were then stained with trypan blue to determine viability. Viable cell numbers in the treated cultures are shown relative to controls
Mentions: To measure any enhanced effect of L. casei extract plus anti-cancer drugs, CaSki and HeLa cells were treated with L. casei extract alone, anti-cancer drugs alone (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM), or L. casei extract plus anti-cancer drugs (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM). All of the anti-cancer drugs inhibited the growth of these cells, but L. casei extract had no additional effect (Fig. 3A, B).

Bottom Line: L. casei extract had no significant effect on the growth rate of the two cell lines.Anti-cancer drugs alone induced growth inhibition, but there was no synergistic effect of L. casei extract on growth inhibition.L. casei extract does not have a potent effect on the viability of cervical cancer cells in vitro.

View Article: PubMed Central - PubMed

Affiliation: Konkuk University Medical Center, Seoul, South Korea.

ABSTRACT

Aim of the study: Lactobacillus casei (L. casei) has been shown to inhibit the proliferation of several types of cancer in vivo, but its effect on cervical cells has not been reported. We incubated cells of the human cervical cell lines Caski and HeLa with extracts of L. casei and investigated its effects on the growth of the cells and possible synergy with anticancer drugs.

Material and methods: Cell-free extracts of L. casei were prepared and purified. Cultures of Caski and HeLa cells adhering to tissue culture plates were treated with L. casei extract. The effects of L. casei extract on the growth of cancer cells and its possible synergy with anti-cancer drugs in cervical cancer cell lines were investigated. The cells were treated with L. casei extract alone, anti-cancer drugs alone [doxorubicin, paclitaxel, 5-fluorouracil (5-FU), and cisplatin], or L. casei extract plus anti-cancer drugs.

Results: L. casei extract had no significant effect on the growth rate of the two cell lines. Anti-cancer drugs alone induced growth inhibition, but there was no synergistic effect of L. casei extract on growth inhibition.

Conclusions: L. casei extract does not have a potent effect on the viability of cervical cancer cells in vitro. In addition, L. casei extract has no synergistic effect on the inhibition of growth of cancer cells in the presence of anti-cancer drugs.

No MeSH data available.


Related in: MedlinePlus