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ERCC1 and RRM1 as a predictive parameter for non-small cell lung, ovarian or pancreas cancer treated with cisplatin and/or gemcitabine.

Ulker M, Duman BB, Sahin B, Gumurdulu D - Contemp Oncol (Pozn) (2015)

Bottom Line: Median survival duration in patients with ovarian cancer showing low ERCC1 and RRM1 expressions was longer than that seen in patients with high expressions.Although no significant correlation was found between ERCC1 and the survival in ovarian cancer (p = 0.183), there was a significant correlation between RRM1 expression and survival in patients with pancreatic cancer (p = 0.005).Our results suggest a predictive value of ERCC1 in lung and ovarian cancers, and also RRM1 in lung and pancreatic cancers.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Medical Faculty, Çukurova University, Turkey.

ABSTRACT

Background: We aimed to investigate the impact of RRM1 and ERCC1 expression on response to cisplatin and/or gemcitabine chemotherapy in patients with lung, ovarian or pancreatic cancer.

Material and methods: Patients with lung, ovarian or pancreatic cancer, who used cisplatin and/or gemcitabine therapy were included; hospital files were examined and RRM1 and ERCC1 expression were evaluated with an immunohistochemical method on tissue cross sections from paraffin blocks of the tumour.

Results: Out of 89 patients, 51%, 30% and 19% had lung, ovarian and pancreatic cancer, respectively. The response rates to the therapy in patients with lung and ovarian cancer having low ERCC1 expression were 62% and 90%, respectively (p = 0.028 and p = 0.044, respectively). No significant association was found between ERCC1 expression and response to therapy in patients with pancreatic cancer (p = 0.354). Therapeutic response rates in patients with lung and pancreatic cancer with low RRM1 expression were 60% and 82%, respectively. Survival rates were higher in patients with lung cancer in which ERCC1 and RRM1 expressions were low. Median survival duration in patients with ovarian cancer showing low ERCC1 and RRM1 expressions was longer than that seen in patients with high expressions. Although no significant correlation was found between ERCC1 and the survival in ovarian cancer (p = 0.183), there was a significant correlation between RRM1 expression and survival in patients with pancreatic cancer (p = 0.005).

Conclusions: Our results suggest a predictive value of ERCC1 in lung and ovarian cancers, and also RRM1 in lung and pancreatic cancers.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical staining in lung, ovarian and pancreatic cancer. A) Cytoplasmic RRM1 +3 staining in lung squamous cell carcinoma. B) Cytoplasmic RRM1 +3 staining in lung adenocarcinoma. C) Cytoplasmic and nuclear RRM1 +3 staining in lung adenocarcinoma. D) Cytoplasmic and nuclear ERCC1 +3 staining in lung squamous cell carcinoma. E) Cytoplasmic RRM1 +3 staining in pancreas cancer. F) Cytoplasmic and nuclear ERCC1 +3 staining in ovarian cancer
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Figure 0001: Immunohistochemical staining in lung, ovarian and pancreatic cancer. A) Cytoplasmic RRM1 +3 staining in lung squamous cell carcinoma. B) Cytoplasmic RRM1 +3 staining in lung adenocarcinoma. C) Cytoplasmic and nuclear RRM1 +3 staining in lung adenocarcinoma. D) Cytoplasmic and nuclear ERCC1 +3 staining in lung squamous cell carcinoma. E) Cytoplasmic RRM1 +3 staining in pancreas cancer. F) Cytoplasmic and nuclear ERCC1 +3 staining in ovarian cancer

Mentions: All pathological specimens were evaluated by the same expert pathologist at the Department of Pathology at Çukurova University. The pathologist was not informed about the clinical and demographic data of the patients. Preparations stained with ERCC1 and RRM1 antibodies were evaluated under a light microscope at × 400 magnification. Cytoplasmic and nuclear staining for ERCC1, and cytoplasmic staining for RRM1 were considered as positive staining. ERCC1 and RRM1 expression of tumour cells was ranked from 0 to 3. No staining in tumour cells was considered as 0, whereas 1–10% staining in tumour cells was +1, 10–50% staining in tumour cells was +2, and > 50% staining in tumour cells was +3 (Fig. 1).


ERCC1 and RRM1 as a predictive parameter for non-small cell lung, ovarian or pancreas cancer treated with cisplatin and/or gemcitabine.

Ulker M, Duman BB, Sahin B, Gumurdulu D - Contemp Oncol (Pozn) (2015)

Immunohistochemical staining in lung, ovarian and pancreatic cancer. A) Cytoplasmic RRM1 +3 staining in lung squamous cell carcinoma. B) Cytoplasmic RRM1 +3 staining in lung adenocarcinoma. C) Cytoplasmic and nuclear RRM1 +3 staining in lung adenocarcinoma. D) Cytoplasmic and nuclear ERCC1 +3 staining in lung squamous cell carcinoma. E) Cytoplasmic RRM1 +3 staining in pancreas cancer. F) Cytoplasmic and nuclear ERCC1 +3 staining in ovarian cancer
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631284&req=5

Figure 0001: Immunohistochemical staining in lung, ovarian and pancreatic cancer. A) Cytoplasmic RRM1 +3 staining in lung squamous cell carcinoma. B) Cytoplasmic RRM1 +3 staining in lung adenocarcinoma. C) Cytoplasmic and nuclear RRM1 +3 staining in lung adenocarcinoma. D) Cytoplasmic and nuclear ERCC1 +3 staining in lung squamous cell carcinoma. E) Cytoplasmic RRM1 +3 staining in pancreas cancer. F) Cytoplasmic and nuclear ERCC1 +3 staining in ovarian cancer
Mentions: All pathological specimens were evaluated by the same expert pathologist at the Department of Pathology at Çukurova University. The pathologist was not informed about the clinical and demographic data of the patients. Preparations stained with ERCC1 and RRM1 antibodies were evaluated under a light microscope at × 400 magnification. Cytoplasmic and nuclear staining for ERCC1, and cytoplasmic staining for RRM1 were considered as positive staining. ERCC1 and RRM1 expression of tumour cells was ranked from 0 to 3. No staining in tumour cells was considered as 0, whereas 1–10% staining in tumour cells was +1, 10–50% staining in tumour cells was +2, and > 50% staining in tumour cells was +3 (Fig. 1).

Bottom Line: Median survival duration in patients with ovarian cancer showing low ERCC1 and RRM1 expressions was longer than that seen in patients with high expressions.Although no significant correlation was found between ERCC1 and the survival in ovarian cancer (p = 0.183), there was a significant correlation between RRM1 expression and survival in patients with pancreatic cancer (p = 0.005).Our results suggest a predictive value of ERCC1 in lung and ovarian cancers, and also RRM1 in lung and pancreatic cancers.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Medical Faculty, Çukurova University, Turkey.

ABSTRACT

Background: We aimed to investigate the impact of RRM1 and ERCC1 expression on response to cisplatin and/or gemcitabine chemotherapy in patients with lung, ovarian or pancreatic cancer.

Material and methods: Patients with lung, ovarian or pancreatic cancer, who used cisplatin and/or gemcitabine therapy were included; hospital files were examined and RRM1 and ERCC1 expression were evaluated with an immunohistochemical method on tissue cross sections from paraffin blocks of the tumour.

Results: Out of 89 patients, 51%, 30% and 19% had lung, ovarian and pancreatic cancer, respectively. The response rates to the therapy in patients with lung and ovarian cancer having low ERCC1 expression were 62% and 90%, respectively (p = 0.028 and p = 0.044, respectively). No significant association was found between ERCC1 expression and response to therapy in patients with pancreatic cancer (p = 0.354). Therapeutic response rates in patients with lung and pancreatic cancer with low RRM1 expression were 60% and 82%, respectively. Survival rates were higher in patients with lung cancer in which ERCC1 and RRM1 expressions were low. Median survival duration in patients with ovarian cancer showing low ERCC1 and RRM1 expressions was longer than that seen in patients with high expressions. Although no significant correlation was found between ERCC1 and the survival in ovarian cancer (p = 0.183), there was a significant correlation between RRM1 expression and survival in patients with pancreatic cancer (p = 0.005).

Conclusions: Our results suggest a predictive value of ERCC1 in lung and ovarian cancers, and also RRM1 in lung and pancreatic cancers.

No MeSH data available.


Related in: MedlinePlus