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Radiation enhancing effects of sanazole and gemcitabine in hypoxic breast and cervical cancer cells in vitro.

Zeng YC, Wu R, Xiao YP, Xin Y, Chi F, Xing R, Xue M, Wang NQ - Contemp Oncol (Pozn) (2015)

Bottom Line: Cell cycle was determined by flow cytometry.Cell surviving fractions were determined by the standard in vitro colony formation assay.This study indicated that the co-administration of the two drugs may result in a beneficial gain in radio-therapy for hypoxic breast cancer and cervical cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, Shengjing Hospital of China Medical University, Shenyang, China ; Cancer Insititute, No. 1 Hospital of China Medical University, Shenyang, China.

ABSTRACT

Aim of the study: Sanazole and gemcitabine have been proven clinically as hypoxic cell radiosensitisers. This study was conducted to determine the radiation enhancing effects of sanazole and gemcitabine when administered together at relevant concentrations into hypoxic human MCF-7 and HeLa cells.

Material and methods: A 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay was used to evaluate the number of surviving cells. Cell cycle was determined by flow cytometry. Cell surviving fractions were determined by the standard in vitro colony formation assay.

Results: The cell colony formation assay indicated that the radiosensitivity of hypoxic MCF-7 and HeLa cells was enhanced by sanazole or gemcitabine. The combination of the two drugs displayed significant radiation enhancing effects at the irradiation doses of 6, 8, and 10 Gy in both cell lines, which were arrested in the S phase.

Conclusions: This study indicated that the co-administration of the two drugs may result in a beneficial gain in radio-therapy for hypoxic breast cancer and cervical cancer.

No MeSH data available.


Related in: MedlinePlus

Colony formation assay: cell survival fraction versus irradiation dose for hypoxic MCF-7 cells with sanazole and/or gemcitabine
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Figure 0001: Colony formation assay: cell survival fraction versus irradiation dose for hypoxic MCF-7 cells with sanazole and/or gemcitabine

Mentions: Figures 1 and 2 show the cell survival curves of MCF-7 and Hela cells. The radiosensitivity of hypoxic MCF-7 and Hela cells was enhanced by sanazole or gemcitabine. The combination of the two drugs also displayed a radiation enhancing effect at the irradiation doses of 6, 8, and 10 Gy in both cell lines. The radiosensitising effect of 5 µM gemcitabine was higher than that of 1 mM sanazole in both MCF-7 and HeLa cells. The radiosensitivity of HeLa cells was affected more than that of MCF-7 cells by the two drugs used either alone or in combination. The survival rate of non-irradiated cells also decreased, mainly due to the cytotoxicity of gemcitabine.


Radiation enhancing effects of sanazole and gemcitabine in hypoxic breast and cervical cancer cells in vitro.

Zeng YC, Wu R, Xiao YP, Xin Y, Chi F, Xing R, Xue M, Wang NQ - Contemp Oncol (Pozn) (2015)

Colony formation assay: cell survival fraction versus irradiation dose for hypoxic MCF-7 cells with sanazole and/or gemcitabine
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631282&req=5

Figure 0001: Colony formation assay: cell survival fraction versus irradiation dose for hypoxic MCF-7 cells with sanazole and/or gemcitabine
Mentions: Figures 1 and 2 show the cell survival curves of MCF-7 and Hela cells. The radiosensitivity of hypoxic MCF-7 and Hela cells was enhanced by sanazole or gemcitabine. The combination of the two drugs also displayed a radiation enhancing effect at the irradiation doses of 6, 8, and 10 Gy in both cell lines. The radiosensitising effect of 5 µM gemcitabine was higher than that of 1 mM sanazole in both MCF-7 and HeLa cells. The radiosensitivity of HeLa cells was affected more than that of MCF-7 cells by the two drugs used either alone or in combination. The survival rate of non-irradiated cells also decreased, mainly due to the cytotoxicity of gemcitabine.

Bottom Line: Cell cycle was determined by flow cytometry.Cell surviving fractions were determined by the standard in vitro colony formation assay.This study indicated that the co-administration of the two drugs may result in a beneficial gain in radio-therapy for hypoxic breast cancer and cervical cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, Shengjing Hospital of China Medical University, Shenyang, China ; Cancer Insititute, No. 1 Hospital of China Medical University, Shenyang, China.

ABSTRACT

Aim of the study: Sanazole and gemcitabine have been proven clinically as hypoxic cell radiosensitisers. This study was conducted to determine the radiation enhancing effects of sanazole and gemcitabine when administered together at relevant concentrations into hypoxic human MCF-7 and HeLa cells.

Material and methods: A 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay was used to evaluate the number of surviving cells. Cell cycle was determined by flow cytometry. Cell surviving fractions were determined by the standard in vitro colony formation assay.

Results: The cell colony formation assay indicated that the radiosensitivity of hypoxic MCF-7 and HeLa cells was enhanced by sanazole or gemcitabine. The combination of the two drugs displayed significant radiation enhancing effects at the irradiation doses of 6, 8, and 10 Gy in both cell lines, which were arrested in the S phase.

Conclusions: This study indicated that the co-administration of the two drugs may result in a beneficial gain in radio-therapy for hypoxic breast cancer and cervical cancer.

No MeSH data available.


Related in: MedlinePlus