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Expression of Th17 cell population regulatory cytokines in laryngeal carcinoma - Preliminary study.

Kopta R, Mochocki M, Morawski P, Brzezińska-Błaszczyk E, Lewy-Trenda I, Student Scientific CircleStarska K - Contemp Oncol (Pozn) (2015)

Bottom Line: Our data demonstrated higher concentrations of IL-23 in patients as compared to controls (p = 0.0001).Our study revealed significant dependences in IL-23 expression on pT (p = 0.04), histological differentiation (p = 0.04), and TFG total score (p = 0.02).Our data did not indicate a relationship between cytokine levels and three- and five-year survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Żeromski Specialist Hospital, Kraków, Poland.

ABSTRACT

Aim of the study: Aim of the study was to evaluate the potential role of regulatory and proinflammatory cytokines IL-23 and IL-17 as Th17 lymphocyte activity markers in relation to invasiveness in laryngeal cancer.

Material and methods: The immunological analysis was conducted in 50 patients treated for squamous cell laryngeal carcinoma and 30 healthy volunteers as controls. The levels of IL-23 and IL-17 in supernatants of purified peripheral blood mononuclear cell cultures were determined by using the enzyme-linked immunosorbent assay (ELISA). The clinicomorphological criteria included pTNM, stage, G, and the total tumour front grading (TFG) score.

Results: Our data demonstrated higher concentrations of IL-23 in patients as compared to controls (p = 0.0001). No statistical difference for IL-17 in these groups was observed. Our study revealed significant dependences in IL-23 expression on pT (p = 0.04), histological differentiation (p = 0.04), and TFG total score (p = 0.02). Advanced tumours (pT3-pT4) with higher grade (G2-G3) and higher invasiveness (> 14 TFG points) were characterised by elevated IL-23 levels in PBMC supernatants. Our data did not indicate a relationship between cytokine levels and three- and five-year survival. However, a tendency towards lower content of IL-23 in PBMC cultures in patients who lived longer than five years after treatment was noted. The relationships between IL-17 level in PBMC cultures and clinicomorphological and prognostic parameters have not been disclosed.

Conclusions: The results of this study suggest the importance of regulatory cytokine IL-23 in determining the aggressive potential of laryngeal carcinomas.

No MeSH data available.


Related in: MedlinePlus

The concentration of IL-17 in the group studied depending on the 3-year and 5-year survival time
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Figure 0002: The concentration of IL-17 in the group studied depending on the 3-year and 5-year survival time

Mentions: The relationship of IL-23 and IL-17 level in supernatants of purified PBMCs with three-year and five-year survival of patients with laryngeal carcinoma was analysed. Statistical analysis did not disclose significant differentiation of both, IL-23 and IL-17 concentration in relation to patient survival. However, a tendency towards lower content of IL-23 in PBMC cultures in patients who lived longer than five years after treatment as well as higher values for IL-17, for whom the survival period was over three years, was noted. The IL-23 and IL-17 levels in PBMC cultures in patients with laryngeal carcinoma and statistical analysis results are shown in Figs. 1 and 2.


Expression of Th17 cell population regulatory cytokines in laryngeal carcinoma - Preliminary study.

Kopta R, Mochocki M, Morawski P, Brzezińska-Błaszczyk E, Lewy-Trenda I, Student Scientific CircleStarska K - Contemp Oncol (Pozn) (2015)

The concentration of IL-17 in the group studied depending on the 3-year and 5-year survival time
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631281&req=5

Figure 0002: The concentration of IL-17 in the group studied depending on the 3-year and 5-year survival time
Mentions: The relationship of IL-23 and IL-17 level in supernatants of purified PBMCs with three-year and five-year survival of patients with laryngeal carcinoma was analysed. Statistical analysis did not disclose significant differentiation of both, IL-23 and IL-17 concentration in relation to patient survival. However, a tendency towards lower content of IL-23 in PBMC cultures in patients who lived longer than five years after treatment as well as higher values for IL-17, for whom the survival period was over three years, was noted. The IL-23 and IL-17 levels in PBMC cultures in patients with laryngeal carcinoma and statistical analysis results are shown in Figs. 1 and 2.

Bottom Line: Our data demonstrated higher concentrations of IL-23 in patients as compared to controls (p = 0.0001).Our study revealed significant dependences in IL-23 expression on pT (p = 0.04), histological differentiation (p = 0.04), and TFG total score (p = 0.02).Our data did not indicate a relationship between cytokine levels and three- and five-year survival.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology, Żeromski Specialist Hospital, Kraków, Poland.

ABSTRACT

Aim of the study: Aim of the study was to evaluate the potential role of regulatory and proinflammatory cytokines IL-23 and IL-17 as Th17 lymphocyte activity markers in relation to invasiveness in laryngeal cancer.

Material and methods: The immunological analysis was conducted in 50 patients treated for squamous cell laryngeal carcinoma and 30 healthy volunteers as controls. The levels of IL-23 and IL-17 in supernatants of purified peripheral blood mononuclear cell cultures were determined by using the enzyme-linked immunosorbent assay (ELISA). The clinicomorphological criteria included pTNM, stage, G, and the total tumour front grading (TFG) score.

Results: Our data demonstrated higher concentrations of IL-23 in patients as compared to controls (p = 0.0001). No statistical difference for IL-17 in these groups was observed. Our study revealed significant dependences in IL-23 expression on pT (p = 0.04), histological differentiation (p = 0.04), and TFG total score (p = 0.02). Advanced tumours (pT3-pT4) with higher grade (G2-G3) and higher invasiveness (> 14 TFG points) were characterised by elevated IL-23 levels in PBMC supernatants. Our data did not indicate a relationship between cytokine levels and three- and five-year survival. However, a tendency towards lower content of IL-23 in PBMC cultures in patients who lived longer than five years after treatment was noted. The relationships between IL-17 level in PBMC cultures and clinicomorphological and prognostic parameters have not been disclosed.

Conclusions: The results of this study suggest the importance of regulatory cytokine IL-23 in determining the aggressive potential of laryngeal carcinomas.

No MeSH data available.


Related in: MedlinePlus