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Dermal type I collagen assessment by digital image analysis.

Brianezi G, Grandi F, Bagatin E, Enokihara MM, Miot HA - An Bras Dermatol (2015)

Bottom Line: However, visual gradation (0 to 4+) has low precision and high subjectivity levels.Automated type I collagen density estimation in the papillary dermis (two techniques) were correlated with visual evaluations (Spearman's rho coefficients of 0.48 and 0.62 (p<0.01)).With regard to the inter-observer repeatability, the four evaluators who used visual classification had an intraclass correlation coefficient (for absolute agreement) of 0.53, while the other two evaluators who used digital analysis (algorithm II) had an intraclass correlation coefficient of 0.97.

View Article: PubMed Central - PubMed

Affiliation: Universidade Estadual Paulista, Botucatu, SP, Brazil.

ABSTRACT
AbstractType I collagen is the main dermal component, and its evaluation is relevant to quantitative studies in dermatopathology. However, visual gradation (0 to 4+) has low precision and high subjectivity levels. This study aimed to develop and validate a digital morphometric analysis technique to estimate type I collagen levels in the papillary dermis. Four evaluators visually quantified (0 to 4+) the density of type I collagen in 63 images of forearm skin biopsies marked by immunohistochemistry and two evaluators analyzed the same images using digital morphometric techniques (RGB split colors (I) and color deconvolution (II)). Automated type I collagen density estimation in the papillary dermis (two techniques) were correlated with visual evaluations (Spearman's rho coefficients of 0.48 and 0.62 (p<0.01)). With regard to the inter-observer repeatability, the four evaluators who used visual classification had an intraclass correlation coefficient (for absolute agreement) of 0.53, while the other two evaluators who used digital analysis (algorithm II) had an intraclass correlation coefficient of 0.97.

No MeSH data available.


Standards of visual gradation for papillary collagen type I (1+ to 4+)
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f01: Standards of visual gradation for papillary collagen type I (1+ to 4+)

Mentions: Qualitative assessments (ranking from zero to 4+) of collagen type I density in thepapillary dermis seen in the images were performed by two senior dermatopathologists (Ev3and Ev4) and two boarded dermatologists (Ev1 and Ev2) (Figure 1).


Dermal type I collagen assessment by digital image analysis.

Brianezi G, Grandi F, Bagatin E, Enokihara MM, Miot HA - An Bras Dermatol (2015)

Standards of visual gradation for papillary collagen type I (1+ to 4+)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4631237&req=5

f01: Standards of visual gradation for papillary collagen type I (1+ to 4+)
Mentions: Qualitative assessments (ranking from zero to 4+) of collagen type I density in thepapillary dermis seen in the images were performed by two senior dermatopathologists (Ev3and Ev4) and two boarded dermatologists (Ev1 and Ev2) (Figure 1).

Bottom Line: However, visual gradation (0 to 4+) has low precision and high subjectivity levels.Automated type I collagen density estimation in the papillary dermis (two techniques) were correlated with visual evaluations (Spearman's rho coefficients of 0.48 and 0.62 (p<0.01)).With regard to the inter-observer repeatability, the four evaluators who used visual classification had an intraclass correlation coefficient (for absolute agreement) of 0.53, while the other two evaluators who used digital analysis (algorithm II) had an intraclass correlation coefficient of 0.97.

View Article: PubMed Central - PubMed

Affiliation: Universidade Estadual Paulista, Botucatu, SP, Brazil.

ABSTRACT
AbstractType I collagen is the main dermal component, and its evaluation is relevant to quantitative studies in dermatopathology. However, visual gradation (0 to 4+) has low precision and high subjectivity levels. This study aimed to develop and validate a digital morphometric analysis technique to estimate type I collagen levels in the papillary dermis. Four evaluators visually quantified (0 to 4+) the density of type I collagen in 63 images of forearm skin biopsies marked by immunohistochemistry and two evaluators analyzed the same images using digital morphometric techniques (RGB split colors (I) and color deconvolution (II)). Automated type I collagen density estimation in the papillary dermis (two techniques) were correlated with visual evaluations (Spearman's rho coefficients of 0.48 and 0.62 (p<0.01)). With regard to the inter-observer repeatability, the four evaluators who used visual classification had an intraclass correlation coefficient (for absolute agreement) of 0.53, while the other two evaluators who used digital analysis (algorithm II) had an intraclass correlation coefficient of 0.97.

No MeSH data available.