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Serum Biopterin and Neopterin Levels as Predictors of Empty Follicles.

Hamuro A, Tachibana D, Misugi T, Katayama H, Ozaki K, Fujino Y, Yoshihiro N, Shintaku H, Koyama M - Jpn Clin Med (2015)

Bottom Line: VEGF and M-CSF levels were measured by enzyme-linked immunosorbent assay.Compared to Group 2, serum and FF levels of neopterin and VEGF and serum levels of M-CSF were significantly increased, and serum and FF levels of biopterin were significantly decreased in Group 1 (P < 0.05 each).Biopterin and neopterin levels showed similar differences in FF and serum of patients with empty follicles.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Osaka City University Graduate School of Medicine, Osaka, Japan.

ABSTRACT

Objective: This study measured serum and follicular fluid (FF) levels of biopterin, neopterin, vascular endothelial growth factor (VEGF), and macrophage colony-stimulating factor (M-CSF) in patients receiving mild ovarian stimulation for oocyte retrieval.

Patients and methods: Infertile patients who underwent ovarian stimulation were divided into the following: Group 1, no oocyte retrieval (n = 12), and Group 2, retrieval of more than four oocytes (n = 13). Median total gonadotropin dose in both groups was 150 IU. Biopterin and neopterin levels were measured using high-performance liquid chromatography. VEGF and M-CSF levels were measured by enzyme-linked immunosorbent assay.

Results: Compared to Group 2, serum and FF levels of neopterin and VEGF and serum levels of M-CSF were significantly increased, and serum and FF levels of biopterin were significantly decreased in Group 1 (P < 0.05 each).

Conclusion: Biopterin and neopterin levels showed similar differences in FF and serum of patients with empty follicles. Decreased biopterin and increased neopterin in serum could predict poor oocyte retrieval.

No MeSH data available.


Related in: MedlinePlus

Serum (A) and FF (B) M-CSF levels in each group. Median (black bars), IQR (boxes), values within 1.5 IQR (whiskers), and values exceeding 1.5 IQR (circles) and 3.0 IQR (asterisks) are shown.
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Related In: Results  -  Collection


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f5-jcm-6-2015-029: Serum (A) and FF (B) M-CSF levels in each group. Median (black bars), IQR (boxes), values within 1.5 IQR (whiskers), and values exceeding 1.5 IQR (circles) and 3.0 IQR (asterisks) are shown.

Mentions: Serum and follicular concentrations in each group are shown in Figures 1–5. Biopterin levels from both serum and FF were significantly higher in Group 2 than in Group 1 (P = 0.002 in serum; P = 0.035 in FF). Conversely, neopterin and VEGF levels from both serum and FF and M-CSF levels from serum were significantly higher in Group 1 than in Group 2 (neopterin: P < 0.001 in serum and P = 0.005 in follicle; VEGF: P = 0.005 in serum and P = 0.035 in follicle; M-CSF: P = 0.007).


Serum Biopterin and Neopterin Levels as Predictors of Empty Follicles.

Hamuro A, Tachibana D, Misugi T, Katayama H, Ozaki K, Fujino Y, Yoshihiro N, Shintaku H, Koyama M - Jpn Clin Med (2015)

Serum (A) and FF (B) M-CSF levels in each group. Median (black bars), IQR (boxes), values within 1.5 IQR (whiskers), and values exceeding 1.5 IQR (circles) and 3.0 IQR (asterisks) are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4631162&req=5

f5-jcm-6-2015-029: Serum (A) and FF (B) M-CSF levels in each group. Median (black bars), IQR (boxes), values within 1.5 IQR (whiskers), and values exceeding 1.5 IQR (circles) and 3.0 IQR (asterisks) are shown.
Mentions: Serum and follicular concentrations in each group are shown in Figures 1–5. Biopterin levels from both serum and FF were significantly higher in Group 2 than in Group 1 (P = 0.002 in serum; P = 0.035 in FF). Conversely, neopterin and VEGF levels from both serum and FF and M-CSF levels from serum were significantly higher in Group 1 than in Group 2 (neopterin: P < 0.001 in serum and P = 0.005 in follicle; VEGF: P = 0.005 in serum and P = 0.035 in follicle; M-CSF: P = 0.007).

Bottom Line: VEGF and M-CSF levels were measured by enzyme-linked immunosorbent assay.Compared to Group 2, serum and FF levels of neopterin and VEGF and serum levels of M-CSF were significantly increased, and serum and FF levels of biopterin were significantly decreased in Group 1 (P < 0.05 each).Biopterin and neopterin levels showed similar differences in FF and serum of patients with empty follicles.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Osaka City University Graduate School of Medicine, Osaka, Japan.

ABSTRACT

Objective: This study measured serum and follicular fluid (FF) levels of biopterin, neopterin, vascular endothelial growth factor (VEGF), and macrophage colony-stimulating factor (M-CSF) in patients receiving mild ovarian stimulation for oocyte retrieval.

Patients and methods: Infertile patients who underwent ovarian stimulation were divided into the following: Group 1, no oocyte retrieval (n = 12), and Group 2, retrieval of more than four oocytes (n = 13). Median total gonadotropin dose in both groups was 150 IU. Biopterin and neopterin levels were measured using high-performance liquid chromatography. VEGF and M-CSF levels were measured by enzyme-linked immunosorbent assay.

Results: Compared to Group 2, serum and FF levels of neopterin and VEGF and serum levels of M-CSF were significantly increased, and serum and FF levels of biopterin were significantly decreased in Group 1 (P < 0.05 each).

Conclusion: Biopterin and neopterin levels showed similar differences in FF and serum of patients with empty follicles. Decreased biopterin and increased neopterin in serum could predict poor oocyte retrieval.

No MeSH data available.


Related in: MedlinePlus