Bat and pig IFN-induced transmembrane protein 3 restrict cell entry by influenza virus and lyssaviruses.
Bottom Line: Ectopically expressed pig and microbat IFITM3 co-localized with transferrin (early endosomes) and CD63 (late endosomes/multivesicular bodies).Pig and microbat IFITM3 restricted cell entry mediated by multiple influenza haemagglutinin subtypes and lyssavirus glycoproteins.In summary, we showed that IFITMs function as potent broad-spectrum antiviral effectors in two mammals - pigs and bats - identified as major reservoirs for emerging viruses.
Affiliation: Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hatfield, UK firstname.lastname@example.org.Show MeSH
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Mentions: Similar to human IFITM3, both pig and microbat IFITM3 had a punctate intracellular distribution following cell fixation and permeabilization (Fig. 2) and co-localized with endocytosed transferrin (early endosomes) and with CD63, a marker for late endosomes/multivesicular bodies (MVBs), but not with the lysosomal marker LAMP1 (Fig. 2). There was an enlargement of CD63-positive structures in cells expressing microbat or human IFITM3 in comparison with the smaller CD63-positive vesicles seen in pig IFITM3-expressing or untransduced A549 cells (Fig. 2b). Enlargement of CD63-containing compartments was most marked in cells containing larger foci of IFITM3–HA staining. Microbat IFITM3–HA sometimes co-localized with CD63 in ‘hollow’ ring-like structures (arrows in Fig. 2b).
Affiliation: Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hatfield, UK email@example.com.