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Chenopodium ambrosioides L. Reduces Synovial Inflammation and Pain in Experimental Osteoarthritis.

Calado GP, Lopes AJ, Costa Junior LM, Lima Fd, Silva LA, Pereira WS, Amaral FM, Garcia JB, Cartágenes Mdo S, Nascimento FR - PLoS ONE (2015)

Bottom Line: After the third day, HCE reduced knee edema.C. ambrosioides HCE was effective in the treatment of OA because it reduced synovial inflammation and behavioral changes due to pain.This effect may be related to the antagonistic effect of ascaridole on the NMDA receptor.

View Article: PubMed Central - PubMed

Affiliation: Health Sciences Graduate Program, Federal University of Maranhao, Biologic and Health Sciences Center, Av. dos Portugueses 1966, São Luís, MA, CEP:65085-580, Brazil.

ABSTRACT
The chronicity of osteoarthritis (OA), characterized by pain and inflammation in the joints, is linked to a glutamate receptor, N-methyl-D-aspartate (NMDA). The use of plant species such as Chenopodium ambrosioides L. (Amaranthaceae) as NMDA antagonists offers a promising perspective. This work aims to analyze the antinociceptive and anti-inflammatory responses of the crude hydroalcoholic extract (HCE) of C. ambrosioides leaves in an experimental OA model. Wistar rats were separated into six groups (n = 24): clean (C), negative control (CTL-), positive control (CTL+), HCE0.5, HCE5 and HCE50. The first group received no intervention. The other groups received an intra-articular injection of sodium monoiodoacetate (MIA) (8 mg/kg) on day 0. After six hours, they were orally treated with saline, Maxicam plus (meloxicam + chondroitin sulfate) and HCE at doses of 0.5 mg/kg, 5 mg/kg and 50 mg/kg, respectively. After three, seven and ten days, clinical evaluations were performed (knee diameter, mechanical allodynia, mechanical hyperalgesia and motor activity). On the tenth day, after euthanasia, synovial fluid and draining lymph node were collected for cellular quantification, and cartilage was collected for histopathological analysis. Finally, molecular docking was performed to evaluate the compatibility of ascaridole, a monoterpene found in HCE, with the NMDA receptor. After the third day, HCE reduced knee edema. HCE5 showed less cellular infiltrate in the cartilage and synovium and lower intensities of allodynia from the third day and of hyperalgesia from the seventh day up to the last treatment day. The HCE5 and HCE50 groups improved in forced walking. In relation to molecular docking, ascaridole showed NMDA receptor binding affinity. C. ambrosioides HCE was effective in the treatment of OA because it reduced synovial inflammation and behavioral changes due to pain. This effect may be related to the antagonistic effect of ascaridole on the NMDA receptor.

No MeSH data available.


Related in: MedlinePlus

Evaluation of the motor activity of animals with OA and treated with Chenopodium ambrosioides HCE.OA was induced by MIA injection (8 mg/kg) in Wistar rat knees. After six hours, the animals received, by oral route, saline (CTL-), Maxicam plus (CTL+) or Chenopodium ambrosioides HCE at doses of 0.5, 5 and 50 mg/kg (HCE0.5, HCE5 and HCE50, respectively). After three, seven and ten days, the motor activity of the animals was measured to evaluate the walking score using a Rotarod device. A shows the kinetics of the animals' walking score, and B shows the percentage improvement in walking on day ten compared to day three. The data are represented as the means± standard errors of the mean. The dashed red line represents the Clean group (without OA and untreated). *p<0.05 compared to CTL- group.
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pone.0141886.g004: Evaluation of the motor activity of animals with OA and treated with Chenopodium ambrosioides HCE.OA was induced by MIA injection (8 mg/kg) in Wistar rat knees. After six hours, the animals received, by oral route, saline (CTL-), Maxicam plus (CTL+) or Chenopodium ambrosioides HCE at doses of 0.5, 5 and 50 mg/kg (HCE0.5, HCE5 and HCE50, respectively). After three, seven and ten days, the motor activity of the animals was measured to evaluate the walking score using a Rotarod device. A shows the kinetics of the animals' walking score, and B shows the percentage improvement in walking on day ten compared to day three. The data are represented as the means± standard errors of the mean. The dashed red line represents the Clean group (without OA and untreated). *p<0.05 compared to CTL- group.

Mentions: Three days after the MIA injection, the animals showed decreases in the forced walking score, as expected. On the seventh day, the CTL-, HCE0.5 and HCE5 groups still showed decreases in the walking score, while the HCE50 and CTL+ groups showed improvements in walking, in the latter case a significant improvement. On the tenth day, all groups treated with HCE had increased scores, with improved walking; however, only the HCE5 group had a significant increase. A similar result was noted for the CTL+ group compared to the CTL- group (Fig 4A). When the percentage walking improvement of the treatments was studied relative to the third day, all treatment groups showed improvements in walking percentage, with the exception of HCE0.5. The improvement was most evident in the HCE5 group, which was close to the CTL+ value (Fig 4B).


Chenopodium ambrosioides L. Reduces Synovial Inflammation and Pain in Experimental Osteoarthritis.

Calado GP, Lopes AJ, Costa Junior LM, Lima Fd, Silva LA, Pereira WS, Amaral FM, Garcia JB, Cartágenes Mdo S, Nascimento FR - PLoS ONE (2015)

Evaluation of the motor activity of animals with OA and treated with Chenopodium ambrosioides HCE.OA was induced by MIA injection (8 mg/kg) in Wistar rat knees. After six hours, the animals received, by oral route, saline (CTL-), Maxicam plus (CTL+) or Chenopodium ambrosioides HCE at doses of 0.5, 5 and 50 mg/kg (HCE0.5, HCE5 and HCE50, respectively). After three, seven and ten days, the motor activity of the animals was measured to evaluate the walking score using a Rotarod device. A shows the kinetics of the animals' walking score, and B shows the percentage improvement in walking on day ten compared to day three. The data are represented as the means± standard errors of the mean. The dashed red line represents the Clean group (without OA and untreated). *p<0.05 compared to CTL- group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4629912&req=5

pone.0141886.g004: Evaluation of the motor activity of animals with OA and treated with Chenopodium ambrosioides HCE.OA was induced by MIA injection (8 mg/kg) in Wistar rat knees. After six hours, the animals received, by oral route, saline (CTL-), Maxicam plus (CTL+) or Chenopodium ambrosioides HCE at doses of 0.5, 5 and 50 mg/kg (HCE0.5, HCE5 and HCE50, respectively). After three, seven and ten days, the motor activity of the animals was measured to evaluate the walking score using a Rotarod device. A shows the kinetics of the animals' walking score, and B shows the percentage improvement in walking on day ten compared to day three. The data are represented as the means± standard errors of the mean. The dashed red line represents the Clean group (without OA and untreated). *p<0.05 compared to CTL- group.
Mentions: Three days after the MIA injection, the animals showed decreases in the forced walking score, as expected. On the seventh day, the CTL-, HCE0.5 and HCE5 groups still showed decreases in the walking score, while the HCE50 and CTL+ groups showed improvements in walking, in the latter case a significant improvement. On the tenth day, all groups treated with HCE had increased scores, with improved walking; however, only the HCE5 group had a significant increase. A similar result was noted for the CTL+ group compared to the CTL- group (Fig 4A). When the percentage walking improvement of the treatments was studied relative to the third day, all treatment groups showed improvements in walking percentage, with the exception of HCE0.5. The improvement was most evident in the HCE5 group, which was close to the CTL+ value (Fig 4B).

Bottom Line: After the third day, HCE reduced knee edema.C. ambrosioides HCE was effective in the treatment of OA because it reduced synovial inflammation and behavioral changes due to pain.This effect may be related to the antagonistic effect of ascaridole on the NMDA receptor.

View Article: PubMed Central - PubMed

Affiliation: Health Sciences Graduate Program, Federal University of Maranhao, Biologic and Health Sciences Center, Av. dos Portugueses 1966, São Luís, MA, CEP:65085-580, Brazil.

ABSTRACT
The chronicity of osteoarthritis (OA), characterized by pain and inflammation in the joints, is linked to a glutamate receptor, N-methyl-D-aspartate (NMDA). The use of plant species such as Chenopodium ambrosioides L. (Amaranthaceae) as NMDA antagonists offers a promising perspective. This work aims to analyze the antinociceptive and anti-inflammatory responses of the crude hydroalcoholic extract (HCE) of C. ambrosioides leaves in an experimental OA model. Wistar rats were separated into six groups (n = 24): clean (C), negative control (CTL-), positive control (CTL+), HCE0.5, HCE5 and HCE50. The first group received no intervention. The other groups received an intra-articular injection of sodium monoiodoacetate (MIA) (8 mg/kg) on day 0. After six hours, they were orally treated with saline, Maxicam plus (meloxicam + chondroitin sulfate) and HCE at doses of 0.5 mg/kg, 5 mg/kg and 50 mg/kg, respectively. After three, seven and ten days, clinical evaluations were performed (knee diameter, mechanical allodynia, mechanical hyperalgesia and motor activity). On the tenth day, after euthanasia, synovial fluid and draining lymph node were collected for cellular quantification, and cartilage was collected for histopathological analysis. Finally, molecular docking was performed to evaluate the compatibility of ascaridole, a monoterpene found in HCE, with the NMDA receptor. After the third day, HCE reduced knee edema. HCE5 showed less cellular infiltrate in the cartilage and synovium and lower intensities of allodynia from the third day and of hyperalgesia from the seventh day up to the last treatment day. The HCE5 and HCE50 groups improved in forced walking. In relation to molecular docking, ascaridole showed NMDA receptor binding affinity. C. ambrosioides HCE was effective in the treatment of OA because it reduced synovial inflammation and behavioral changes due to pain. This effect may be related to the antagonistic effect of ascaridole on the NMDA receptor.

No MeSH data available.


Related in: MedlinePlus