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Neocryptotanshinone inhibits lipopolysaccharide-induced inflammation in RAW264.7 macrophages by suppression of NF-κB and iNOS signaling pathways.

Wu C, Zhao W, Zhang X, Chen X - Acta Pharm Sin B (2015)

Bottom Line: MTT results showed that NCTS partly reversed LPS-induced cytotoxicity.Moreover, NCTS could decrease LPS-induced nitric oxide (NO) production.In conclusion, these data demonstrated that NCTS showed anti-inflammatory effect by suppression of NF-κB and iNOS signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.

ABSTRACT
Neocryptotanshinone (NCTS) is a natural product isolated from traditional Chinese herb Salvia miltiorrhiza Bunge. In this study, we investigated its anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) cells. MTT results showed that NCTS partly reversed LPS-induced cytotoxicity. Real-time PCR results showed that NCTS suppressed LPS-induced mRNA expression of inflammatory cytokines, including tumor necrosis factor α (TNFα), interleukin-6 (IL-6) and interleukin-1β (IL-1β). Moreover, NCTS could decrease LPS-induced nitric oxide (NO) production. Western blotting results showed that NCTS could down-regulate LPS-induced expression of inducible nitric oxide synthase (iNOS), p-IκBα, p-IKKβ and p-NF-κB p65 without affecting cyclooxygenase-2 (COX-2). In addition, NCTS inhibited LPS-induced p-NF-κB p65 nuclear translocation. In conclusion, these data demonstrated that NCTS showed anti-inflammatory effect by suppression of NF-κB and iNOS signaling pathways.

No MeSH data available.


Related in: MedlinePlus

Effect of NCTS on LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages. Cells were treated with LPS with or without NCTS co-incubation for 24 h. The protein expression of iNOS and COX-2 was determined by western blotting. Data were presented as the means±SD of three independent experiments. ##P<0.01, #P<0.05 vs. the control group; *P<0.05 vs. LPS-stimulated group. NCTS, Neocryptotanshinone; Cont, Control group.
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f0015: Effect of NCTS on LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages. Cells were treated with LPS with or without NCTS co-incubation for 24 h. The protein expression of iNOS and COX-2 was determined by western blotting. Data were presented as the means±SD of three independent experiments. ##P<0.01, #P<0.05 vs. the control group; *P<0.05 vs. LPS-stimulated group. NCTS, Neocryptotanshinone; Cont, Control group.

Mentions: iNOS and cyclooxygenase (COX-2) were responsible for the production of NO and prostaglandin E2 (PGE2), two important inflammatory mediators. LPS treatment significantly up-regulated the protein expression of iNOS and COX-2. NCTS, at the concentration of 10 μmol/L and 20 μmol/L, could decrease the expression of iNOS remarkably (Fig. 3A). However, NCTS showed no effect on LPS-induced up-regulation of COX-2 at all three tested concentrations (Fig. 3B).


Neocryptotanshinone inhibits lipopolysaccharide-induced inflammation in RAW264.7 macrophages by suppression of NF-κB and iNOS signaling pathways.

Wu C, Zhao W, Zhang X, Chen X - Acta Pharm Sin B (2015)

Effect of NCTS on LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages. Cells were treated with LPS with or without NCTS co-incubation for 24 h. The protein expression of iNOS and COX-2 was determined by western blotting. Data were presented as the means±SD of three independent experiments. ##P<0.01, #P<0.05 vs. the control group; *P<0.05 vs. LPS-stimulated group. NCTS, Neocryptotanshinone; Cont, Control group.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4629269&req=5

f0015: Effect of NCTS on LPS-induced protein expression of iNOS and COX-2 in RAW264.7 macrophages. Cells were treated with LPS with or without NCTS co-incubation for 24 h. The protein expression of iNOS and COX-2 was determined by western blotting. Data were presented as the means±SD of three independent experiments. ##P<0.01, #P<0.05 vs. the control group; *P<0.05 vs. LPS-stimulated group. NCTS, Neocryptotanshinone; Cont, Control group.
Mentions: iNOS and cyclooxygenase (COX-2) were responsible for the production of NO and prostaglandin E2 (PGE2), two important inflammatory mediators. LPS treatment significantly up-regulated the protein expression of iNOS and COX-2. NCTS, at the concentration of 10 μmol/L and 20 μmol/L, could decrease the expression of iNOS remarkably (Fig. 3A). However, NCTS showed no effect on LPS-induced up-regulation of COX-2 at all three tested concentrations (Fig. 3B).

Bottom Line: MTT results showed that NCTS partly reversed LPS-induced cytotoxicity.Moreover, NCTS could decrease LPS-induced nitric oxide (NO) production.In conclusion, these data demonstrated that NCTS showed anti-inflammatory effect by suppression of NF-κB and iNOS signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.

ABSTRACT
Neocryptotanshinone (NCTS) is a natural product isolated from traditional Chinese herb Salvia miltiorrhiza Bunge. In this study, we investigated its anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) cells. MTT results showed that NCTS partly reversed LPS-induced cytotoxicity. Real-time PCR results showed that NCTS suppressed LPS-induced mRNA expression of inflammatory cytokines, including tumor necrosis factor α (TNFα), interleukin-6 (IL-6) and interleukin-1β (IL-1β). Moreover, NCTS could decrease LPS-induced nitric oxide (NO) production. Western blotting results showed that NCTS could down-regulate LPS-induced expression of inducible nitric oxide synthase (iNOS), p-IκBα, p-IKKβ and p-NF-κB p65 without affecting cyclooxygenase-2 (COX-2). In addition, NCTS inhibited LPS-induced p-NF-κB p65 nuclear translocation. In conclusion, these data demonstrated that NCTS showed anti-inflammatory effect by suppression of NF-κB and iNOS signaling pathways.

No MeSH data available.


Related in: MedlinePlus