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Neocryptotanshinone inhibits lipopolysaccharide-induced inflammation in RAW264.7 macrophages by suppression of NF- κ B and iNOS signaling pathways

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ABSTRACT

Neocryptotanshinone (NCTS) is a natural product isolated from traditional Chinese herb Salvia miltiorrhiza Bunge. In this study, we investigated its anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) cells. MTT results showed that NCTS partly reversed LPS-induced cytotoxicity. Real-time PCR results showed that NCTS suppressed LPS-induced mRNA expression of inflammatory cytokines, including tumor necrosis factor α (TNFα), interleukin-6 (IL-6) and interleukin-1β (IL-1β). Moreover, NCTS could decrease LPS-induced nitric oxide (NO) production. Western blotting results showed that NCTS could down-regulate LPS-induced expression of inducible nitric oxide synthase (iNOS), p-IκBα, p-IKKβ and p-NF-κB p65 without affecting cyclooxygenase-2 (COX-2). In addition, NCTS inhibited LPS-induced p-NF-κB p65 nuclear translocation. In conclusion, these data demonstrated that NCTS showed anti-inflammatory effect by suppression of NF-κB and iNOS signaling pathways.

No MeSH data available.


Effects of NCTS on LPS-induced mRNA expression of TNFα, IL-6 and IL-1β in RAW264.7 macrophages. Cells were treated with LPS with or without NCTS co-incubation for 24 h. The total RNA was prepared and the mRNA expression of TNFα, IL-6 and IL-1β were measured with RT-PCR. Data were presented as the means±SD of three independent experiments. ##P<0.01 vs. the control group; *P<0.05 vs. LPS-stimulated group. NCTS, Neocryptotanshinone; Cont, Control group.
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f0010: Effects of NCTS on LPS-induced mRNA expression of TNFα, IL-6 and IL-1β in RAW264.7 macrophages. Cells were treated with LPS with or without NCTS co-incubation for 24 h. The total RNA was prepared and the mRNA expression of TNFα, IL-6 and IL-1β were measured with RT-PCR. Data were presented as the means±SD of three independent experiments. ##P<0.01 vs. the control group; *P<0.05 vs. LPS-stimulated group. NCTS, Neocryptotanshinone; Cont, Control group.

Mentions: To evaluate the effect of NCTS on pro-inflammatory cytokines, we examined the mRNA expression of TNFα, IL-6 and IL-1β. LPS treatment dramatically increased the mRNA expression of TNFα, IL-6 and IL-1β. NCTS could significantly suppresses the LPS-induced mRNA expression of TNFα, IL-6 and IL-1β. (Fig. 2).


Neocryptotanshinone inhibits lipopolysaccharide-induced inflammation in RAW264.7 macrophages by suppression of NF- κ B and iNOS signaling pathways
Effects of NCTS on LPS-induced mRNA expression of TNFα, IL-6 and IL-1β in RAW264.7 macrophages. Cells were treated with LPS with or without NCTS co-incubation for 24 h. The total RNA was prepared and the mRNA expression of TNFα, IL-6 and IL-1β were measured with RT-PCR. Data were presented as the means±SD of three independent experiments. ##P<0.01 vs. the control group; *P<0.05 vs. LPS-stimulated group. NCTS, Neocryptotanshinone; Cont, Control group.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

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f0010: Effects of NCTS on LPS-induced mRNA expression of TNFα, IL-6 and IL-1β in RAW264.7 macrophages. Cells were treated with LPS with or without NCTS co-incubation for 24 h. The total RNA was prepared and the mRNA expression of TNFα, IL-6 and IL-1β were measured with RT-PCR. Data were presented as the means±SD of three independent experiments. ##P<0.01 vs. the control group; *P<0.05 vs. LPS-stimulated group. NCTS, Neocryptotanshinone; Cont, Control group.
Mentions: To evaluate the effect of NCTS on pro-inflammatory cytokines, we examined the mRNA expression of TNFα, IL-6 and IL-1β. LPS treatment dramatically increased the mRNA expression of TNFα, IL-6 and IL-1β. NCTS could significantly suppresses the LPS-induced mRNA expression of TNFα, IL-6 and IL-1β. (Fig. 2).

View Article: PubMed Central

ABSTRACT

Neocryptotanshinone (NCTS) is a natural product isolated from traditional Chinese herb Salvia miltiorrhiza Bunge. In this study, we investigated its anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) cells. MTT results showed that NCTS partly reversed LPS-induced cytotoxicity. Real-time PCR results showed that NCTS suppressed LPS-induced mRNA expression of inflammatory cytokines, including tumor necrosis factor &alpha; (TNF&alpha;), interleukin-6 (IL-6) and interleukin-1&beta; (IL-1&beta;). Moreover, NCTS could decrease LPS-induced nitric oxide (NO) production. Western blotting results showed that NCTS could down-regulate LPS-induced expression of inducible nitric oxide synthase (iNOS), p-I&kappa;B&alpha;, p-IKK&beta; and p-NF-&kappa;B p65 without affecting cyclooxygenase-2 (COX-2). In addition, NCTS inhibited LPS-induced p-NF-&kappa;B p65 nuclear translocation. In conclusion, these data demonstrated that NCTS showed anti-inflammatory effect by suppression of NF-&kappa;B and iNOS signaling pathways.

No MeSH data available.