Limits...
Progesterone Enhanced Remyelination in the Mouse Corpus Callosum after Cuprizone Induced Demyelination.

Kashani IR, Hedayatpour A, Pasbakhsh P, Kafami L, Khallaghi B, Malek F - Iran J Med Sci (2015)

Bottom Line: Luxol-fast blue staining revealed enhanced remyelination in the progesterone group when compared with the placebo group.Densitometry measurements of immunoblots demonstrated that MBP and PLP proteins contents were significantly increased in the progesterone group compared with the placebo group.Flow cytometry and IHC analysis showed increases in Olig2 and O4 cells in the progesterone group compared with the placebo group.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Progesterone as a sex steroid hormone is thought to affect and prevent demyelination, but its role in promoting myelin repair is far less investigated. In this study, remyelinating potential of progesterone in corpus callosum was evaluated on an experimental model of MS.

Methods: In this experimental study, adult male C57BL/6 mice were fed with 0.2% (w/w) cuprizone in ground breeder chow ad libitum for 6 weeks. At day zero, after cuprizone removal, mice were divided randomly into two groups: (a) placebo group, which received saline pellet implant, (b) progesterone group, which received progesterone pellet implant. Some mice of the same age were fed with their normal diet to serve as the healthy control group. Two weeks after progesterone administration, Myelin content was assessed by Luxol-fast blue staining. The myelin basic protein (MBP) and proteolipid protein (PLP) expression were assessed using Western blot analysis and the changes in the number of oligodendrocytes and oligodendroglial progenitor cells were assessed by immunohistochemistry (IHC) and flow cytometry.

Results: Luxol-fast blue staining revealed enhanced remyelination in the progesterone group when compared with the placebo group. Densitometry measurements of immunoblots demonstrated that MBP and PLP proteins contents were significantly increased in the progesterone group compared with the placebo group. Flow cytometry and IHC analysis showed increases in Olig2 and O4 cells in the progesterone group compared with the placebo group.

Conclusion: Overall, our results indicate that progesterone treatment can stimulate myelin production and that it may provide a feasible and practical way for remyelination in diseases such as multiple sclerosis.

No MeSH data available.


Related in: MedlinePlus

Protein expression of myelin basic protein (MBP) and proteolipid protein (PLP) were measured by Western blot and β actin was used as housekeeping control. A: The representative Western blot pictures of MBP and PLP protein in corpus callosum of the healthy control, placebo and progesterone administration mice. B: Bar chart showing the relative quantities of MBP and PLP measured densitometrically from the Western blots in different groups. The value represented here as mean±SEM of 3 mice in each group (*P<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4628141&req=5

Figure 2: Protein expression of myelin basic protein (MBP) and proteolipid protein (PLP) were measured by Western blot and β actin was used as housekeeping control. A: The representative Western blot pictures of MBP and PLP protein in corpus callosum of the healthy control, placebo and progesterone administration mice. B: Bar chart showing the relative quantities of MBP and PLP measured densitometrically from the Western blots in different groups. The value represented here as mean±SEM of 3 mice in each group (*P<0.05).

Mentions: Densitometric measurements of immunoblots demonstrated that MBP and PLP contents were significantly lower in all treated groups compared with the healthy control group (figure 2A). In the progesterone pellet implantation group, after two weeks of hormone administration, there were significant increase in MBP (0.72±0.1; P≤0.05) contents compared with the placebo group (0.24±0.1; P≤0.05) but less than the healthy control group (0.95±0.06; P≤0.05) (figure 2B). The PLP contents were significantly increased in progesterone receiving group (0.8±0.09; P≤0.05) compared with the placebo group (0.1±0.03; P≤0.05) but less than the healthy control group (0.99±0.08; P≤0.05).


Progesterone Enhanced Remyelination in the Mouse Corpus Callosum after Cuprizone Induced Demyelination.

Kashani IR, Hedayatpour A, Pasbakhsh P, Kafami L, Khallaghi B, Malek F - Iran J Med Sci (2015)

Protein expression of myelin basic protein (MBP) and proteolipid protein (PLP) were measured by Western blot and β actin was used as housekeeping control. A: The representative Western blot pictures of MBP and PLP protein in corpus callosum of the healthy control, placebo and progesterone administration mice. B: Bar chart showing the relative quantities of MBP and PLP measured densitometrically from the Western blots in different groups. The value represented here as mean±SEM of 3 mice in each group (*P<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4628141&req=5

Figure 2: Protein expression of myelin basic protein (MBP) and proteolipid protein (PLP) were measured by Western blot and β actin was used as housekeeping control. A: The representative Western blot pictures of MBP and PLP protein in corpus callosum of the healthy control, placebo and progesterone administration mice. B: Bar chart showing the relative quantities of MBP and PLP measured densitometrically from the Western blots in different groups. The value represented here as mean±SEM of 3 mice in each group (*P<0.05).
Mentions: Densitometric measurements of immunoblots demonstrated that MBP and PLP contents were significantly lower in all treated groups compared with the healthy control group (figure 2A). In the progesterone pellet implantation group, after two weeks of hormone administration, there were significant increase in MBP (0.72±0.1; P≤0.05) contents compared with the placebo group (0.24±0.1; P≤0.05) but less than the healthy control group (0.95±0.06; P≤0.05) (figure 2B). The PLP contents were significantly increased in progesterone receiving group (0.8±0.09; P≤0.05) compared with the placebo group (0.1±0.03; P≤0.05) but less than the healthy control group (0.99±0.08; P≤0.05).

Bottom Line: Luxol-fast blue staining revealed enhanced remyelination in the progesterone group when compared with the placebo group.Densitometry measurements of immunoblots demonstrated that MBP and PLP proteins contents were significantly increased in the progesterone group compared with the placebo group.Flow cytometry and IHC analysis showed increases in Olig2 and O4 cells in the progesterone group compared with the placebo group.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Progesterone as a sex steroid hormone is thought to affect and prevent demyelination, but its role in promoting myelin repair is far less investigated. In this study, remyelinating potential of progesterone in corpus callosum was evaluated on an experimental model of MS.

Methods: In this experimental study, adult male C57BL/6 mice were fed with 0.2% (w/w) cuprizone in ground breeder chow ad libitum for 6 weeks. At day zero, after cuprizone removal, mice were divided randomly into two groups: (a) placebo group, which received saline pellet implant, (b) progesterone group, which received progesterone pellet implant. Some mice of the same age were fed with their normal diet to serve as the healthy control group. Two weeks after progesterone administration, Myelin content was assessed by Luxol-fast blue staining. The myelin basic protein (MBP) and proteolipid protein (PLP) expression were assessed using Western blot analysis and the changes in the number of oligodendrocytes and oligodendroglial progenitor cells were assessed by immunohistochemistry (IHC) and flow cytometry.

Results: Luxol-fast blue staining revealed enhanced remyelination in the progesterone group when compared with the placebo group. Densitometry measurements of immunoblots demonstrated that MBP and PLP proteins contents were significantly increased in the progesterone group compared with the placebo group. Flow cytometry and IHC analysis showed increases in Olig2 and O4 cells in the progesterone group compared with the placebo group.

Conclusion: Overall, our results indicate that progesterone treatment can stimulate myelin production and that it may provide a feasible and practical way for remyelination in diseases such as multiple sclerosis.

No MeSH data available.


Related in: MedlinePlus