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Stable transformation and expression of GhEXPA8 fiber expansin gene to improve fiber length and micronaire value in cotton

View Article: PubMed Central

ABSTRACT

Cotton fiber is multigenic trait controlled by number of genes. Previous studies suggest that one of these genes may be responsible for switching cotton fiber growth on and off to influence the fiber quality produced from a cotton seed. In the present study, the Gossypium hirsutum GhEXPA8 fiber expansin gene was introduced into local cotton variety NIAB 846 by using an Agrobacterium-mediated gene transformation. The neomycin phosphotransferase (NPTII) gene was used as a selection marker for screening of putative transgenic cotton plants. Integration and expression of the fiber expansin gene in cotton plants was confirmed with molecular techniques including Southern blot analyses, real-time PCR. Cellulose assay was used for measurement of cellulose contents of transgenic cotton fiber. The data collected from 3 years of field performance of the transgenic cotton plants expressing GhEXPA8 showed that significant improvement has been made in fiber lengths and micronaire values as compared to control G. hirsutum variety NIAB 846 cotton plants. Statistical techniques were also used for analysis of fiber and agronomic characteristics. The results of this study support improvement of cotton fiber through genetic modification.

No MeSH data available.


Comparison of cellulose contents of transgenic and control cotton fiber along with standard control. Plants in groups 1 to 6 were showed three different samples of cellulose. Blue color represent the amount of cellulose from Avicel PH-101pure cellulose, red color represent the amount of cellulose obtained from untransformed cotton, green color indicated the depicts the presence of cellulose in transgenic cotton.
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Figure 6: Comparison of cellulose contents of transgenic and control cotton fiber along with standard control. Plants in groups 1 to 6 were showed three different samples of cellulose. Blue color represent the amount of cellulose from Avicel PH-101pure cellulose, red color represent the amount of cellulose obtained from untransformed cotton, green color indicated the depicts the presence of cellulose in transgenic cotton.

Mentions: The quantity of cellulose in the cotton fibers after genetic transformation were increased from 20 days post-anthesis (DPA), with improved changeability between the transgenic and parental cotton fibers. In this study, we compared the cellulose content of transgenic cotton fibers and control cotton fibers. Plant line 6 (GhEXPA8-15) produced 3-folds more cellulose, plant line 5 (GhEXPA8-13) produced 2.5-fold more cellulose, plant line 1 (GhEXPA8-1) produced 2-fold more cellulose, and plant line 2 (GhEXPA8-4) produced 1.5-fold more cellulose than the control lines. The cellulose levels of two transgenic lines, 3 (GhEXPA8-5) and 4 (GhEXPA8-11), were slightly higher than the control line, but lower than the other transgenic lines. The transgenic plant lines 1, 4, 5, and 6 had higher cellulose content than the control line, which positively affected one or more fiber quality parameters (Figure 6).


Stable transformation and expression of GhEXPA8 fiber expansin gene to improve fiber length and micronaire value in cotton
Comparison of cellulose contents of transgenic and control cotton fiber along with standard control. Plants in groups 1 to 6 were showed three different samples of cellulose. Blue color represent the amount of cellulose from Avicel PH-101pure cellulose, red color represent the amount of cellulose obtained from untransformed cotton, green color indicated the depicts the presence of cellulose in transgenic cotton.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4628126&req=5

Figure 6: Comparison of cellulose contents of transgenic and control cotton fiber along with standard control. Plants in groups 1 to 6 were showed three different samples of cellulose. Blue color represent the amount of cellulose from Avicel PH-101pure cellulose, red color represent the amount of cellulose obtained from untransformed cotton, green color indicated the depicts the presence of cellulose in transgenic cotton.
Mentions: The quantity of cellulose in the cotton fibers after genetic transformation were increased from 20 days post-anthesis (DPA), with improved changeability between the transgenic and parental cotton fibers. In this study, we compared the cellulose content of transgenic cotton fibers and control cotton fibers. Plant line 6 (GhEXPA8-15) produced 3-folds more cellulose, plant line 5 (GhEXPA8-13) produced 2.5-fold more cellulose, plant line 1 (GhEXPA8-1) produced 2-fold more cellulose, and plant line 2 (GhEXPA8-4) produced 1.5-fold more cellulose than the control lines. The cellulose levels of two transgenic lines, 3 (GhEXPA8-5) and 4 (GhEXPA8-11), were slightly higher than the control line, but lower than the other transgenic lines. The transgenic plant lines 1, 4, 5, and 6 had higher cellulose content than the control line, which positively affected one or more fiber quality parameters (Figure 6).

View Article: PubMed Central

ABSTRACT

Cotton fiber is multigenic trait controlled by number of genes. Previous studies suggest that one of these genes may be responsible for switching cotton fiber growth on and off to influence the fiber quality produced from a cotton seed. In the present study, the Gossypium hirsutum GhEXPA8 fiber expansin gene was introduced into local cotton variety NIAB 846 by using an Agrobacterium-mediated gene transformation. The neomycin phosphotransferase (NPTII) gene was used as a selection marker for screening of putative transgenic cotton plants. Integration and expression of the fiber expansin gene in cotton plants was confirmed with molecular techniques including Southern blot analyses, real-time PCR. Cellulose assay was used for measurement of cellulose contents of transgenic cotton fiber. The data collected from 3 years of field performance of the transgenic cotton plants expressing GhEXPA8 showed that significant improvement has been made in fiber lengths and micronaire values as compared to control G. hirsutum variety NIAB 846 cotton plants. Statistical techniques were also used for analysis of fiber and agronomic characteristics. The results of this study support improvement of cotton fiber through genetic modification.

No MeSH data available.