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Opsonic function of sialic acid specific lectin in freshwater crab Paratelphusa jacquemontii.

Denis M, Thayappan K, Ramasamy SM, Munusamy A - Springerplus (2015)

Bottom Line: The flowcytometry observation affirmed the enhancement of phagocytosis by Pjlec coated hemocyte.The mechanism of phagocytosis and induction were dependent on specific recognition of the erythrocyte by the multivalent binding site of the lectin protein, and the elicitation of the immune response was a function of the sialoglycan surface.The pathway of the challenge suggested that after entry of nonself recognition by lectin was followed by induction and activation of phagocytosis by opsonic binding of the lectin.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai, 600025 India.

ABSTRACT
The sialic acid specific humoral lectin, Pjlec of the freshwater crab Paratelphusa jacquemontii was investigated for its opsonin function with rabbit erythrocyte as target cell for phagocytosis by the crab's hemocyte. The untreated or trypsin treated erythrocyte induced lectin response after challenge however failed when treated with neuraminidase evidently indicating glycan dependency for elicited immune response. Our observation of in vitro phagocytosis of the erythrocyte untreated or coated with serum, clarified serum appeared to be recognized and engulfed by hemocytes but when coated with isolated lectin Pjlec, the response was elicited. Moreover, with trypsin treated erythrocyte the response remained unchanged but neuraminidase or O-glycosidase treatment eliminated the response reaction. This suggested the sialic acid specific reaction of lectin with the erythrocyte and was essential for recognition to allow the lectin Pjlec to act as an opsonin. The flowcytometry observation affirmed the enhancement of phagocytosis by Pjlec coated hemocyte. The efficiency of in vitro hemolysis of Pjlec coated erythrocyte with hemocyte when compared to untreated erythrocyte with or without hemocyte also established the opsonic function of the lectin. The mechanism of phagocytosis and induction were dependent on specific recognition of the erythrocyte by the multivalent binding site of the lectin protein, and the elicitation of the immune response was a function of the sialoglycan surface. The pathway of the challenge suggested that after entry of nonself recognition by lectin was followed by induction and activation of phagocytosis by opsonic binding of the lectin.

No MeSH data available.


Related in: MedlinePlus

Hemolysis of untreated and Pjlec coated rabbit erythrocyte with or without hemocytes from the hemolymph of Paratelphusa jacquemontii. Hemolysis was determined from hemoglobin liberated at 5, 10, 15, 30 min and 1 h and percentage of hemolysis evaluated. Data represents average of five determinations. A Hemolysis % of Pjlec coated rabbit erythrocyte. B Hemolysis % of rabbit erythrocyte with hemocytes. C Hemolysis % of Pjlec coated rabbit erythrocyte with hemocytes
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Fig2: Hemolysis of untreated and Pjlec coated rabbit erythrocyte with or without hemocytes from the hemolymph of Paratelphusa jacquemontii. Hemolysis was determined from hemoglobin liberated at 5, 10, 15, 30 min and 1 h and percentage of hemolysis evaluated. Data represents average of five determinations. A Hemolysis % of Pjlec coated rabbit erythrocyte. B Hemolysis % of rabbit erythrocyte with hemocytes. C Hemolysis % of Pjlec coated rabbit erythrocyte with hemocytes

Mentions: The percentage of hemolysis as recorded for hemocytes, lectin Pjlec and hemocytes with erythrocyte or lectin coated erythrocyte is represented graphically in Fig. 2. Lectin Pjlec or hemocytes had weak hemolytic effect on the erythrocyte. The lectin Pjlec coated erythrocyte with hemocytes facilitated hemolysis of the erythrocyte in comparison to the untreated erythrocytes with hemocytes (p < 0.05).Fig. 2


Opsonic function of sialic acid specific lectin in freshwater crab Paratelphusa jacquemontii.

Denis M, Thayappan K, Ramasamy SM, Munusamy A - Springerplus (2015)

Hemolysis of untreated and Pjlec coated rabbit erythrocyte with or without hemocytes from the hemolymph of Paratelphusa jacquemontii. Hemolysis was determined from hemoglobin liberated at 5, 10, 15, 30 min and 1 h and percentage of hemolysis evaluated. Data represents average of five determinations. A Hemolysis % of Pjlec coated rabbit erythrocyte. B Hemolysis % of rabbit erythrocyte with hemocytes. C Hemolysis % of Pjlec coated rabbit erythrocyte with hemocytes
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4628046&req=5

Fig2: Hemolysis of untreated and Pjlec coated rabbit erythrocyte with or without hemocytes from the hemolymph of Paratelphusa jacquemontii. Hemolysis was determined from hemoglobin liberated at 5, 10, 15, 30 min and 1 h and percentage of hemolysis evaluated. Data represents average of five determinations. A Hemolysis % of Pjlec coated rabbit erythrocyte. B Hemolysis % of rabbit erythrocyte with hemocytes. C Hemolysis % of Pjlec coated rabbit erythrocyte with hemocytes
Mentions: The percentage of hemolysis as recorded for hemocytes, lectin Pjlec and hemocytes with erythrocyte or lectin coated erythrocyte is represented graphically in Fig. 2. Lectin Pjlec or hemocytes had weak hemolytic effect on the erythrocyte. The lectin Pjlec coated erythrocyte with hemocytes facilitated hemolysis of the erythrocyte in comparison to the untreated erythrocytes with hemocytes (p < 0.05).Fig. 2

Bottom Line: The flowcytometry observation affirmed the enhancement of phagocytosis by Pjlec coated hemocyte.The mechanism of phagocytosis and induction were dependent on specific recognition of the erythrocyte by the multivalent binding site of the lectin protein, and the elicitation of the immune response was a function of the sialoglycan surface.The pathway of the challenge suggested that after entry of nonself recognition by lectin was followed by induction and activation of phagocytosis by opsonic binding of the lectin.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai, 600025 India.

ABSTRACT
The sialic acid specific humoral lectin, Pjlec of the freshwater crab Paratelphusa jacquemontii was investigated for its opsonin function with rabbit erythrocyte as target cell for phagocytosis by the crab's hemocyte. The untreated or trypsin treated erythrocyte induced lectin response after challenge however failed when treated with neuraminidase evidently indicating glycan dependency for elicited immune response. Our observation of in vitro phagocytosis of the erythrocyte untreated or coated with serum, clarified serum appeared to be recognized and engulfed by hemocytes but when coated with isolated lectin Pjlec, the response was elicited. Moreover, with trypsin treated erythrocyte the response remained unchanged but neuraminidase or O-glycosidase treatment eliminated the response reaction. This suggested the sialic acid specific reaction of lectin with the erythrocyte and was essential for recognition to allow the lectin Pjlec to act as an opsonin. The flowcytometry observation affirmed the enhancement of phagocytosis by Pjlec coated hemocyte. The efficiency of in vitro hemolysis of Pjlec coated erythrocyte with hemocyte when compared to untreated erythrocyte with or without hemocyte also established the opsonic function of the lectin. The mechanism of phagocytosis and induction were dependent on specific recognition of the erythrocyte by the multivalent binding site of the lectin protein, and the elicitation of the immune response was a function of the sialoglycan surface. The pathway of the challenge suggested that after entry of nonself recognition by lectin was followed by induction and activation of phagocytosis by opsonic binding of the lectin.

No MeSH data available.


Related in: MedlinePlus