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Psychrophilic pseudomonas in antarctic freshwater lake at stornes peninsula, larsemann hills over east Antarctica.

Chauhan A, Bharti PK, Goyal P, Varma A, Jindal T - Springerplus (2015)

Bottom Line: Psychrophillic counts were found in the range of 12 cfu to 1.6 × 10(2) cfu in all the samples.MPN Coliform per 100 ml was found to be absent in all the samples.Pseudomonas sp. was found in ST-2 lake water sample as characteristics colonies (Optimum Growth) were observed on selective media at 22 and 25 °C.

View Article: PubMed Central - PubMed

Affiliation: Amity Institute of Environmental Toxicology, Safety and Management, Amity University, Sector-125, Noida, Uttar Pradesh India.

ABSTRACT
The Larsemann Hills is an ice-free area of approximately 50 km(2), located halfway between the Vestfold Hills and the Amery Ice Shelf on the south-eastern coast of Prydz Bay, Princess Elizabeth Land, East Antarctica (69º30'S, 76º19'58″E). The ice-free area consists of two major peninsulas (Stornes and Broknes), four minor peninsulas, and approximately 130 islands. The Larsemann Hills area contains more than 150 lakes at different Islands and Peninsulas. Nine lake water samples were collected in a gamma sterilized bottles and were kept in an ice pack to prevent any changes in the microbial flora of the samples during the transportation. The water samples were transported to the lab in vertical position maintaining the temperature 1-4 °C with ice pack enveloped conditions. Samples were studied for Psychrophilic bacterial count, Pseudomonas spp., Staphylococcus aureus, Salmonella and Total MPN Coliform per 100 ml. Psychrophillic counts were found in the range of 12 cfu to 1.6 × 10(2) cfu in all the samples. MPN Coliform per 100 ml was found to be absent in all the samples. No growth and characteristics colonies observed when tested for Salmonella and S.aureus. Pseudomonas sp. was found in ST-2 lake water sample as characteristics colonies (Optimum Growth) were observed on selective media at 22 and 25 °C. Further several biochemical tests were also performed to confirm the presence of this Potential Psychrophilic Pseudomonas sp. for its further application in Science and Technology.

No MeSH data available.


Related in: MedlinePlus

Characteristics colonies on Cetrimide agar (a selective agar media)
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Fig3: Characteristics colonies on Cetrimide agar (a selective agar media)

Mentions: The present research study was carried out to know the microbiological contamination in Antarctic freshwater lake at stornes peninsula, larsemann hills over east Antarctica. Data of collected sample is summarized in Additional file 1: Table S1 and Additional file 2: Table S2. Psychrophillic counts were found in the range of 12 cfu to 1.6 × 102 cfu in all the samples. MPN Coliform per 100 ml was found to be absent as No Growth in presumptive test was observed, hence sample discontinued for further studies. No any growth and characteristics colonies observed when tested for Salmonella and S. aureus.Pseudomonas sp. was found in ST-2 lake water sample as characteristics colonies (optimum growth) were observed on selective media at 22 and 25 °C. Isolated Pseudomonas was further study for its morphological identification. The isolate was identified Gram-negative rod shaped in Gram-staining slide under Laboratory Microscope. Several biochemical tests were carried out for further identification of isolated strain. The observation of all biochemical test of Pseudomonas isolate is given in Additional file 1: Table S1; Figs. 1, 2, 3, 4, 5, 6 and 7. A positive strain of Pseudomonas aeruginosa MTCC 1688 was also used as a positive control to ensure the laboratory condition during biochemical identification isolated strain. Our study is supported by the research work done by Zhang et al. (2008). They had reported the homology analysis of isolates from Antarctica and identified several close representative group of microorganisms. They also studied the colony morphological characteristics of isolated microorganisms and found that mostly isolates were growing at 4 °C which also an indicator of presence of Pstchrophilic microorganisms in Antarctica.Fig. 2


Psychrophilic pseudomonas in antarctic freshwater lake at stornes peninsula, larsemann hills over east Antarctica.

Chauhan A, Bharti PK, Goyal P, Varma A, Jindal T - Springerplus (2015)

Characteristics colonies on Cetrimide agar (a selective agar media)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4627980&req=5

Fig3: Characteristics colonies on Cetrimide agar (a selective agar media)
Mentions: The present research study was carried out to know the microbiological contamination in Antarctic freshwater lake at stornes peninsula, larsemann hills over east Antarctica. Data of collected sample is summarized in Additional file 1: Table S1 and Additional file 2: Table S2. Psychrophillic counts were found in the range of 12 cfu to 1.6 × 102 cfu in all the samples. MPN Coliform per 100 ml was found to be absent as No Growth in presumptive test was observed, hence sample discontinued for further studies. No any growth and characteristics colonies observed when tested for Salmonella and S. aureus.Pseudomonas sp. was found in ST-2 lake water sample as characteristics colonies (optimum growth) were observed on selective media at 22 and 25 °C. Isolated Pseudomonas was further study for its morphological identification. The isolate was identified Gram-negative rod shaped in Gram-staining slide under Laboratory Microscope. Several biochemical tests were carried out for further identification of isolated strain. The observation of all biochemical test of Pseudomonas isolate is given in Additional file 1: Table S1; Figs. 1, 2, 3, 4, 5, 6 and 7. A positive strain of Pseudomonas aeruginosa MTCC 1688 was also used as a positive control to ensure the laboratory condition during biochemical identification isolated strain. Our study is supported by the research work done by Zhang et al. (2008). They had reported the homology analysis of isolates from Antarctica and identified several close representative group of microorganisms. They also studied the colony morphological characteristics of isolated microorganisms and found that mostly isolates were growing at 4 °C which also an indicator of presence of Pstchrophilic microorganisms in Antarctica.Fig. 2

Bottom Line: Psychrophillic counts were found in the range of 12 cfu to 1.6 × 10(2) cfu in all the samples.MPN Coliform per 100 ml was found to be absent in all the samples.Pseudomonas sp. was found in ST-2 lake water sample as characteristics colonies (Optimum Growth) were observed on selective media at 22 and 25 °C.

View Article: PubMed Central - PubMed

Affiliation: Amity Institute of Environmental Toxicology, Safety and Management, Amity University, Sector-125, Noida, Uttar Pradesh India.

ABSTRACT
The Larsemann Hills is an ice-free area of approximately 50 km(2), located halfway between the Vestfold Hills and the Amery Ice Shelf on the south-eastern coast of Prydz Bay, Princess Elizabeth Land, East Antarctica (69º30'S, 76º19'58″E). The ice-free area consists of two major peninsulas (Stornes and Broknes), four minor peninsulas, and approximately 130 islands. The Larsemann Hills area contains more than 150 lakes at different Islands and Peninsulas. Nine lake water samples were collected in a gamma sterilized bottles and were kept in an ice pack to prevent any changes in the microbial flora of the samples during the transportation. The water samples were transported to the lab in vertical position maintaining the temperature 1-4 °C with ice pack enveloped conditions. Samples were studied for Psychrophilic bacterial count, Pseudomonas spp., Staphylococcus aureus, Salmonella and Total MPN Coliform per 100 ml. Psychrophillic counts were found in the range of 12 cfu to 1.6 × 10(2) cfu in all the samples. MPN Coliform per 100 ml was found to be absent in all the samples. No growth and characteristics colonies observed when tested for Salmonella and S.aureus. Pseudomonas sp. was found in ST-2 lake water sample as characteristics colonies (Optimum Growth) were observed on selective media at 22 and 25 °C. Further several biochemical tests were also performed to confirm the presence of this Potential Psychrophilic Pseudomonas sp. for its further application in Science and Technology.

No MeSH data available.


Related in: MedlinePlus