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Identification of novel post-transcriptional features in olfactory receptor family mRNAs.

Shum EY, Espinoza JL, Ramaiah M, Wilkinson MF - Nucleic Acids Res. (2015)

Bottom Line: Using RNA-seq analysis coupled with analysis of pre-existing databases, we found that Olfr mRNAs have several atypical features suggesting that post-transcriptional regulation impacts their expression.All of these novel properties correlated with higher Olfr expression.Together, our results suggest that the Olfr gene family has encountered unusual selective forces in neural cells that have driven them to acquire unique post-transcriptional regulatory features.

View Article: PubMed Central - PubMed

Affiliation: Department of Reproductive Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0695, USA.

No MeSH data available.


Related in: MedlinePlus

Most Olfr transcripts have short 3′ UTRs. (A, B) Boxplots of 3′ UTR length. (C, D) Boxplots of 5′ UTR length. *P < 0.05, **P < 0.01, ****P < 0.0001; n.s. not statistically significant.
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Figure 2: Most Olfr transcripts have short 3′ UTRs. (A, B) Boxplots of 3′ UTR length. (C, D) Boxplots of 5′ UTR length. *P < 0.05, **P < 0.01, ****P < 0.0001; n.s. not statistically significant.

Mentions: To understand how Olfr mRNA characteristics potentially impact their expression, we analyzed Olfr mRNAs we defined by RNA-seq (Figure 2, Supplementary Figure S1A–B, Supplementary Table S3), as Olfr trancripts on publically available databases such as Ensembl and RefSeq have inaccuracies. Unless otherwise noted, all data analysis below was performed in Olfr mRNAs we identified through our RNA-seq analysis. As a basis of comparison, we examined two other gene groups: (i) Gpcr genes encoding 92 non-OLFR GPCR proteins defined by the International Union of Basic and Clinical Pharmacology Database [IUPHAR-DB] (43) that we found were significantly expressed in the OE, based on our RNA-seq analysis, and (ii) Ctrl genes, a randomly selected group of 587 non-Gpcr genes expressed in the OE.


Identification of novel post-transcriptional features in olfactory receptor family mRNAs.

Shum EY, Espinoza JL, Ramaiah M, Wilkinson MF - Nucleic Acids Res. (2015)

Most Olfr transcripts have short 3′ UTRs. (A, B) Boxplots of 3′ UTR length. (C, D) Boxplots of 5′ UTR length. *P < 0.05, **P < 0.01, ****P < 0.0001; n.s. not statistically significant.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4627058&req=5

Figure 2: Most Olfr transcripts have short 3′ UTRs. (A, B) Boxplots of 3′ UTR length. (C, D) Boxplots of 5′ UTR length. *P < 0.05, **P < 0.01, ****P < 0.0001; n.s. not statistically significant.
Mentions: To understand how Olfr mRNA characteristics potentially impact their expression, we analyzed Olfr mRNAs we defined by RNA-seq (Figure 2, Supplementary Figure S1A–B, Supplementary Table S3), as Olfr trancripts on publically available databases such as Ensembl and RefSeq have inaccuracies. Unless otherwise noted, all data analysis below was performed in Olfr mRNAs we identified through our RNA-seq analysis. As a basis of comparison, we examined two other gene groups: (i) Gpcr genes encoding 92 non-OLFR GPCR proteins defined by the International Union of Basic and Clinical Pharmacology Database [IUPHAR-DB] (43) that we found were significantly expressed in the OE, based on our RNA-seq analysis, and (ii) Ctrl genes, a randomly selected group of 587 non-Gpcr genes expressed in the OE.

Bottom Line: Using RNA-seq analysis coupled with analysis of pre-existing databases, we found that Olfr mRNAs have several atypical features suggesting that post-transcriptional regulation impacts their expression.All of these novel properties correlated with higher Olfr expression.Together, our results suggest that the Olfr gene family has encountered unusual selective forces in neural cells that have driven them to acquire unique post-transcriptional regulatory features.

View Article: PubMed Central - PubMed

Affiliation: Department of Reproductive Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0695, USA.

No MeSH data available.


Related in: MedlinePlus