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The Effects of Targeted Deliveries of Lovastatin and Tocotrienol on Ossification-Related Gene Expressions in Fracture Healing in an Osteoporosis Rat Model.

Ibrahim N', Mohamed N, Soelaiman IN, Shuid AN - Int J Environ Res Public Health (2015)

Bottom Line: After 4 weeks, the gene expressions were measured.Group VI showed significantly higher gene expressions of osteocalcin, BMP-2, VEGF-α, and RUNX-2 compared to Group II.In conclusion, combined treatment of lovastatin and tocotrienol upregulated the expression of genes related to fracture healing.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia. nurulizzah88@gmail.com.

ABSTRACT
Osteoporotic drugs are used to prevent fragility fractures, but their role in fracture healing still remains unknown. Thus, alternative agents with suitable mode of delivery are needed to promote fracture healing. This study was performed to investigate the effects of direct deliveries of lovastatin and tocotrienol to fracture sites on ossification-related gene expression in fracture healing in a postmenopausal osteoporosis model. Forty-eight Sprague Dawley female rats were divided into six groups. Group I comprised the sham-operated rats, while Groups II-VI were ovariectomized rats. After 8 weeks, the right tibiae of all rats were fractured and stabilized. Group I and Group II were given two single injections of lovastatin and tocotrienol carriers. Group III was given an estrogen preparation at 64.5 µg/kg daily via oral gavages. Group IV was injected with lovastatin particles (750 µg/kg), while Group V was injected with tocotrienol particles (60 mg/kg). Group VI received two single injections of 750 µg/kg lovastatin particles and 60 mg/kg tocotrienol particles. After 4 weeks, the gene expressions were measured. Group VI showed significantly higher gene expressions of osteocalcin, BMP-2, VEGF-α, and RUNX-2 compared to Group II. In conclusion, combined treatment of lovastatin and tocotrienol upregulated the expression of genes related to fracture healing.

No MeSH data available.


Related in: MedlinePlus

RUNX-2 Gene Expression. Samples were normalized to GAPDH MFI (Median Fluorescence Intensity). Values were expressed as mean ± SEM. Group I: sham-operated group, Group II: ovariectomized control group, Group III: ovariectomized+Estrogen, Group IV: ovariectomized+Lovastatin, Group V: ovariectomized+Tocotrienol, Group VI: ovariectomized+Tocotrienol+Lovastatin. * Indicates a significant difference (p < 0.05) compared with the group II. # Indicates a significant difference (p < 0.05) compared with the group III.
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ijerph-12-12958-f004: RUNX-2 Gene Expression. Samples were normalized to GAPDH MFI (Median Fluorescence Intensity). Values were expressed as mean ± SEM. Group I: sham-operated group, Group II: ovariectomized control group, Group III: ovariectomized+Estrogen, Group IV: ovariectomized+Lovastatin, Group V: ovariectomized+Tocotrienol, Group VI: ovariectomized+Tocotrienol+Lovastatin. * Indicates a significant difference (p < 0.05) compared with the group II. # Indicates a significant difference (p < 0.05) compared with the group III.

Mentions: The Runx-2 gene expression of the tibial bones was significantly higher in Group VI when compared to Group II and Group III (p < 0.05). There were no other significant differences in the RUNX-2 gene expression levels among the various treatment groups (Figure 4).


The Effects of Targeted Deliveries of Lovastatin and Tocotrienol on Ossification-Related Gene Expressions in Fracture Healing in an Osteoporosis Rat Model.

Ibrahim N', Mohamed N, Soelaiman IN, Shuid AN - Int J Environ Res Public Health (2015)

RUNX-2 Gene Expression. Samples were normalized to GAPDH MFI (Median Fluorescence Intensity). Values were expressed as mean ± SEM. Group I: sham-operated group, Group II: ovariectomized control group, Group III: ovariectomized+Estrogen, Group IV: ovariectomized+Lovastatin, Group V: ovariectomized+Tocotrienol, Group VI: ovariectomized+Tocotrienol+Lovastatin. * Indicates a significant difference (p < 0.05) compared with the group II. # Indicates a significant difference (p < 0.05) compared with the group III.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4627010&req=5

ijerph-12-12958-f004: RUNX-2 Gene Expression. Samples were normalized to GAPDH MFI (Median Fluorescence Intensity). Values were expressed as mean ± SEM. Group I: sham-operated group, Group II: ovariectomized control group, Group III: ovariectomized+Estrogen, Group IV: ovariectomized+Lovastatin, Group V: ovariectomized+Tocotrienol, Group VI: ovariectomized+Tocotrienol+Lovastatin. * Indicates a significant difference (p < 0.05) compared with the group II. # Indicates a significant difference (p < 0.05) compared with the group III.
Mentions: The Runx-2 gene expression of the tibial bones was significantly higher in Group VI when compared to Group II and Group III (p < 0.05). There were no other significant differences in the RUNX-2 gene expression levels among the various treatment groups (Figure 4).

Bottom Line: After 4 weeks, the gene expressions were measured.Group VI showed significantly higher gene expressions of osteocalcin, BMP-2, VEGF-α, and RUNX-2 compared to Group II.In conclusion, combined treatment of lovastatin and tocotrienol upregulated the expression of genes related to fracture healing.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia. nurulizzah88@gmail.com.

ABSTRACT
Osteoporotic drugs are used to prevent fragility fractures, but their role in fracture healing still remains unknown. Thus, alternative agents with suitable mode of delivery are needed to promote fracture healing. This study was performed to investigate the effects of direct deliveries of lovastatin and tocotrienol to fracture sites on ossification-related gene expression in fracture healing in a postmenopausal osteoporosis model. Forty-eight Sprague Dawley female rats were divided into six groups. Group I comprised the sham-operated rats, while Groups II-VI were ovariectomized rats. After 8 weeks, the right tibiae of all rats were fractured and stabilized. Group I and Group II were given two single injections of lovastatin and tocotrienol carriers. Group III was given an estrogen preparation at 64.5 µg/kg daily via oral gavages. Group IV was injected with lovastatin particles (750 µg/kg), while Group V was injected with tocotrienol particles (60 mg/kg). Group VI received two single injections of 750 µg/kg lovastatin particles and 60 mg/kg tocotrienol particles. After 4 weeks, the gene expressions were measured. Group VI showed significantly higher gene expressions of osteocalcin, BMP-2, VEGF-α, and RUNX-2 compared to Group II. In conclusion, combined treatment of lovastatin and tocotrienol upregulated the expression of genes related to fracture healing.

No MeSH data available.


Related in: MedlinePlus