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Changes in Levels of Seminal Nitric Oxide Synthase, Macrophage Migration Inhibitory Factor, Sperm DNA Integrity and Caspase-3 in Fertile Men after Scrotal Heat Stress.

Zhang MH, Zhang AD, Shi ZD, Wang LG, Qiu Y - PLoS ONE (2015)

Bottom Line: Seminal NO and NOS contents were determined by nitrate reduction method.After three months of the SHS, various parameters recovered to the level before SHS.WBC in semen showed a positively significant correlation with the levels of NO, NOS, MIF and Caspase-3 activity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Birth Regulation and Control Technology of National Health and Family Planning Commission of China, Key Laboratory for Improving Birth Outcome Technique, Shandong Provincial Family Planning Institute of Science and Technology, 69 Yuhan Road, Jinan, Shandong, 250002, China.

ABSTRACT

Background: This study observes changes in levels of seminal nitric oxide (NO), nitric oxide synthase (NOS), macrophage migration inhibitory factor (MIF), sperm DNA integrity, chromatin condensation and Caspase-3in adult healthy men after scrotal heat stress (SHS).

Methods: Exposure of the scrotum of 25 healthy male volunteers locally at 40-43°C SHS belt warming 40 min each day for successive 2 d per week. The course of SHS was continuously 3 months. Routine semen analysis, hypo-osmotic swelling (HOS) test, Aniline blue (AB) staining, HOS/AB and terminal deoxynucleotidyl transferase-mediated d UDP nick-end labeling (TUNEL) were carried out before, during and after SHS. Seminal NO and NOS contents were determined by nitrate reduction method. The activated Caspase-3 levels of spermatozoa and MIF in seminal plasma were measured by the enzyme-linked immunosorbent assay (ELISA) method. Statistical significance between mean values was determined using statistical ANOVA tests.

Results: The mean parameters of sperm concentration, motile and progressive motile sperm and normal morphological sperm were significantly decreased in groups during SHS 1, 2 and 3 months compared with those in groups of pre-SHS (P<0.001). Statistically significant differences of sperm DNA fragmentation, normal sperm membrane, and Caspase-3 activity as well as the level of NO, NOS and MIF in semen were observed between the groups before SHS and after SHS 3 months and the groups during SHS 1, 2 and 3 months (P<0.001). After three months of the SHS, various parameters recovered to the level before SHS. WBC in semen showed a positively significant correlation with the levels of NO, NOS, MIF and Caspase-3 activity. The percentage of abnormal sperm by using the test of HOS showed a positively significant correlation with that of HOS/AB.

Conclusions: The continuously constant SHS can impact the semen quality and sperm DNA and chromatin, which may be contributed to the high level of NO, NOS, MIF and Caspase-3 by SHS.

No MeSH data available.


Related in: MedlinePlus

The percentage of sperm DNA fragmentation (%) with TUNEL assay, abnormal sperm chromatin condensation (%) with HOS/AB, normal sperm membrane and vitality (%) with HOS, as well as Caspase-3 activity (U/106 sperms), and levels of NO (μmol/L), NOS (U/ml) and MIF (ng/ml) in seminal plasma were compared before, during and after the use of SHS in 25 subjects.From SPSS 13.0. Mean ± SEM.
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pone.0141320.g004: The percentage of sperm DNA fragmentation (%) with TUNEL assay, abnormal sperm chromatin condensation (%) with HOS/AB, normal sperm membrane and vitality (%) with HOS, as well as Caspase-3 activity (U/106 sperms), and levels of NO (μmol/L), NOS (U/ml) and MIF (ng/ml) in seminal plasma were compared before, during and after the use of SHS in 25 subjects.From SPSS 13.0. Mean ± SEM.

Mentions: Statistically significant differences of sperm DNA fragmentation, normal sperm membrane and vitality, Caspase-3 activity, and levels of NO, NOS and MIF were observed between the groups before SHS and the groups of during SHS 1, 2 and 3 months (P<0.001). The prevalence of abnormal sperm DNA, abnormal sperm chromatin condensation, normal sperm membrane and vitality, and Caspase-3 activity did not show any statistically significant difference between the groups before SHS and after SHS 3 months (P>0.05). Fig 4 shows the percentage of sperm DNA fragmentation with TUNEL assay, abnormal sperm chromatin condensation with HOS/AB, normal sperm membrane and vitality with HOS, as well as Caspase-3 activity, and levels of NO, NOS and MIF in seminal plasma were compared before, during and after the use of SHS in 25 subjects.


Changes in Levels of Seminal Nitric Oxide Synthase, Macrophage Migration Inhibitory Factor, Sperm DNA Integrity and Caspase-3 in Fertile Men after Scrotal Heat Stress.

Zhang MH, Zhang AD, Shi ZD, Wang LG, Qiu Y - PLoS ONE (2015)

The percentage of sperm DNA fragmentation (%) with TUNEL assay, abnormal sperm chromatin condensation (%) with HOS/AB, normal sperm membrane and vitality (%) with HOS, as well as Caspase-3 activity (U/106 sperms), and levels of NO (μmol/L), NOS (U/ml) and MIF (ng/ml) in seminal plasma were compared before, during and after the use of SHS in 25 subjects.From SPSS 13.0. Mean ± SEM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4626044&req=5

pone.0141320.g004: The percentage of sperm DNA fragmentation (%) with TUNEL assay, abnormal sperm chromatin condensation (%) with HOS/AB, normal sperm membrane and vitality (%) with HOS, as well as Caspase-3 activity (U/106 sperms), and levels of NO (μmol/L), NOS (U/ml) and MIF (ng/ml) in seminal plasma were compared before, during and after the use of SHS in 25 subjects.From SPSS 13.0. Mean ± SEM.
Mentions: Statistically significant differences of sperm DNA fragmentation, normal sperm membrane and vitality, Caspase-3 activity, and levels of NO, NOS and MIF were observed between the groups before SHS and the groups of during SHS 1, 2 and 3 months (P<0.001). The prevalence of abnormal sperm DNA, abnormal sperm chromatin condensation, normal sperm membrane and vitality, and Caspase-3 activity did not show any statistically significant difference between the groups before SHS and after SHS 3 months (P>0.05). Fig 4 shows the percentage of sperm DNA fragmentation with TUNEL assay, abnormal sperm chromatin condensation with HOS/AB, normal sperm membrane and vitality with HOS, as well as Caspase-3 activity, and levels of NO, NOS and MIF in seminal plasma were compared before, during and after the use of SHS in 25 subjects.

Bottom Line: Seminal NO and NOS contents were determined by nitrate reduction method.After three months of the SHS, various parameters recovered to the level before SHS.WBC in semen showed a positively significant correlation with the levels of NO, NOS, MIF and Caspase-3 activity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Birth Regulation and Control Technology of National Health and Family Planning Commission of China, Key Laboratory for Improving Birth Outcome Technique, Shandong Provincial Family Planning Institute of Science and Technology, 69 Yuhan Road, Jinan, Shandong, 250002, China.

ABSTRACT

Background: This study observes changes in levels of seminal nitric oxide (NO), nitric oxide synthase (NOS), macrophage migration inhibitory factor (MIF), sperm DNA integrity, chromatin condensation and Caspase-3in adult healthy men after scrotal heat stress (SHS).

Methods: Exposure of the scrotum of 25 healthy male volunteers locally at 40-43°C SHS belt warming 40 min each day for successive 2 d per week. The course of SHS was continuously 3 months. Routine semen analysis, hypo-osmotic swelling (HOS) test, Aniline blue (AB) staining, HOS/AB and terminal deoxynucleotidyl transferase-mediated d UDP nick-end labeling (TUNEL) were carried out before, during and after SHS. Seminal NO and NOS contents were determined by nitrate reduction method. The activated Caspase-3 levels of spermatozoa and MIF in seminal plasma were measured by the enzyme-linked immunosorbent assay (ELISA) method. Statistical significance between mean values was determined using statistical ANOVA tests.

Results: The mean parameters of sperm concentration, motile and progressive motile sperm and normal morphological sperm were significantly decreased in groups during SHS 1, 2 and 3 months compared with those in groups of pre-SHS (P<0.001). Statistically significant differences of sperm DNA fragmentation, normal sperm membrane, and Caspase-3 activity as well as the level of NO, NOS and MIF in semen were observed between the groups before SHS and after SHS 3 months and the groups during SHS 1, 2 and 3 months (P<0.001). After three months of the SHS, various parameters recovered to the level before SHS. WBC in semen showed a positively significant correlation with the levels of NO, NOS, MIF and Caspase-3 activity. The percentage of abnormal sperm by using the test of HOS showed a positively significant correlation with that of HOS/AB.

Conclusions: The continuously constant SHS can impact the semen quality and sperm DNA and chromatin, which may be contributed to the high level of NO, NOS, MIF and Caspase-3 by SHS.

No MeSH data available.


Related in: MedlinePlus