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No Effect of Host Species on Phenoloxidase Activity in a Mycophagous Beetle.

Formica V, Chan AK - PLoS ONE (2015)

Bottom Line: The host species individuals experience have profound effects on immune response in many species of insects.While there was significant variance among individuals in PO activity, there were surprisingly no significant differences in PO activity among subpopulations, beetles living on different host species, or between the sexes; there was also no effect of body size.Our results suggest that other factors such as age, genotype, disease prevalence, or natal environment may be generating variance among individuals in PO activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Swarthmore College, Swarthmore, Pennsylvania, United States of America.

ABSTRACT
Ecological immunology is an interdisciplinary field that helps elucidate interactions between the environment and immune response. The host species individuals experience have profound effects on immune response in many species of insects. However, this conclusion comes from studies of herbivorous insects even though species of mycophagous insects also inhabit many different host species. The goal of this study was to determine if fungal host species as well as individual, sex, body size, and host patch predict one aspect of immune function, phenoloxidase activity (PO). We sampled a metapopulation of Bolitotherus cornutus, a mycophagous beetle in southwestern Virginia. B. cornutus live on three species of fungus that differ in nutritional quality, social environment, and density. A filter paper phenoloxidase assay was used to quantify phenoloxidase activity. Overall, PO activity was significantly repeatable among individuals (0.57) in adult B. cornutus. While there was significant variance among individuals in PO activity, there were surprisingly no significant differences in PO activity among subpopulations, beetles living on different host species, or between the sexes; there was also no effect of body size. Our results suggest that other factors such as age, genotype, disease prevalence, or natal environment may be generating variance among individuals in PO activity.

No MeSH data available.


There is no correlation between the darkness of the hemolymph placed only in buffer and the darkness of hemolymph included in the immune assay (L-Dopa & Buffer; F1, 28 = 1.08, P = 0.31).
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pone.0141167.g001: There is no correlation between the darkness of the hemolymph placed only in buffer and the darkness of hemolymph included in the immune assay (L-Dopa & Buffer; F1, 28 = 1.08, P = 0.31).

Mentions: We found no correlation between darkness of hemolymph in buffer and darkness of hemolymph in the phenoloxidase immunoassay (F1, 28 = 1.08, P = 0.31; Fig 1) suggesting that the differences in darkness among samples was due to the reaction between PO activity and the L-DOPA and not simply due to darkening quinones in the defensive secretion.


No Effect of Host Species on Phenoloxidase Activity in a Mycophagous Beetle.

Formica V, Chan AK - PLoS ONE (2015)

There is no correlation between the darkness of the hemolymph placed only in buffer and the darkness of hemolymph included in the immune assay (L-Dopa & Buffer; F1, 28 = 1.08, P = 0.31).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4625955&req=5

pone.0141167.g001: There is no correlation between the darkness of the hemolymph placed only in buffer and the darkness of hemolymph included in the immune assay (L-Dopa & Buffer; F1, 28 = 1.08, P = 0.31).
Mentions: We found no correlation between darkness of hemolymph in buffer and darkness of hemolymph in the phenoloxidase immunoassay (F1, 28 = 1.08, P = 0.31; Fig 1) suggesting that the differences in darkness among samples was due to the reaction between PO activity and the L-DOPA and not simply due to darkening quinones in the defensive secretion.

Bottom Line: The host species individuals experience have profound effects on immune response in many species of insects.While there was significant variance among individuals in PO activity, there were surprisingly no significant differences in PO activity among subpopulations, beetles living on different host species, or between the sexes; there was also no effect of body size.Our results suggest that other factors such as age, genotype, disease prevalence, or natal environment may be generating variance among individuals in PO activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Swarthmore College, Swarthmore, Pennsylvania, United States of America.

ABSTRACT
Ecological immunology is an interdisciplinary field that helps elucidate interactions between the environment and immune response. The host species individuals experience have profound effects on immune response in many species of insects. However, this conclusion comes from studies of herbivorous insects even though species of mycophagous insects also inhabit many different host species. The goal of this study was to determine if fungal host species as well as individual, sex, body size, and host patch predict one aspect of immune function, phenoloxidase activity (PO). We sampled a metapopulation of Bolitotherus cornutus, a mycophagous beetle in southwestern Virginia. B. cornutus live on three species of fungus that differ in nutritional quality, social environment, and density. A filter paper phenoloxidase assay was used to quantify phenoloxidase activity. Overall, PO activity was significantly repeatable among individuals (0.57) in adult B. cornutus. While there was significant variance among individuals in PO activity, there were surprisingly no significant differences in PO activity among subpopulations, beetles living on different host species, or between the sexes; there was also no effect of body size. Our results suggest that other factors such as age, genotype, disease prevalence, or natal environment may be generating variance among individuals in PO activity.

No MeSH data available.